Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,232	C→T	P183P (CCG→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,232	0	C	T	100.0%	82.8 / NA	26	P183P (CCG→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

GCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTG  >  NC_000913/64992‑65449                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            gCCGTTTTTAAAGCCGTGCTCGCGCCACTCCCGCTCACTGTTATCGCGCCCCAGCCGCAGCGGCCTACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGc                                                                                                                                                                                                                    >  2:248482/1‑250 (MQ=255) gCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCa                                                                                                                                                                                                                   <  1:62970/251‑1 (MQ=255)                     cgcgCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGcc                                                                                                                                                                                                 <  2:79912/249‑1 (MQ=255)                       cgcCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGc                                                                                                                                                                                              >  1:101126/1‑250 (MQ=255)                           aCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATATACGATGCGCTGCCCGCAGCCt                                                                                                                                                                                         >  2:233411/1‑251 (MQ=255)                                aGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTcc                                                                                                                                                                                      >  2:71398/1‑249 (MQ=255)                                              gcgcCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACa                                                                                                                                                                      <  2:320263/251‑1 (MQ=255)                                                     agccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTGGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCGCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGTGGTACAGCTCAcc                                                                                                                                                               <  2:26500/248‑1 (MQ=255)                                                          cAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGc                                                                                                                                                          <  1:278941/251‑1 (MQ=255)                                                                                          gcagcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATAAAT‑AGAAACCCACTTg                                                                                                                         >  1:145142/1‑251 (MQ=255)                                                                                                  cGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGGTGGCAAACCAGGCGTCGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTATTAAAGTTCAAAATCGAGCGCGCAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTACAGGCCGATGCAGTACAGCTCACCGTGGCGGATGGTTGCACTATCCATCACAAACCC‑CATGAGcggcgg                                                                                                                 >  2:285818/1‑251 (MQ=255)                                                                                                                caccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCCATATCAAT‑AGAAACCCACTTGAGCGGCGGGCGaaaggtggg                                                                                                     >  2:17236/1‑241 (MQ=255)                                                                                                                        tCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑ttt                                                                                           <  1:100665/250‑1 (MQ=255)                                                                                                                                   atCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATATCAAT‑AGAAAACCACTTGAGCGGCGGACGAGAGTCGGGGTGCGG‑GTTCAGAAggggctt                                                                               >  2:128281/1‑247 (MQ=255)                                                                                                                                     cacaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAATCGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATCAAT‑AGAAACACACTTGAGCAGCGGGCGGTAGATGGGATGCGGGTTTC‑GACGGGGATTa                                                                              >  2:221791/1‑250 (MQ=255)                                                                                                                                                       ggCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGc                                                            <  2:51423/250‑1 (MQ=255)                                                                                                                                                       ggCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCa                                                           <  1:169402/251‑1 (MQ=255)                                                                                                                                                           aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                       <  1:84992/251‑1 (MQ=255)                                                                                                                                                             ccAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCAc                                                     <  1:74289/251‑1 (MQ=255)                                                                                                                                                              cAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑ATAAACCCACTTGAGCGGCGGACGCTTATCGGGATGCGG‑TTTCAGACGGGCATTAAAGATAGCGCCATTGTGCATATCAcc                                                    >  1:6345/1‑251 (MQ=255)                                                                                                                                                                  cGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCCATATCAAT‑AAAAACCCACTTGAGCGGCGGGCGATAGTCGGGATGGGG‑GTTCAGACGGGCCTTAACGAAAACGCCATTGTGCataaaccccccc                                                >  2:170992/1‑242 (MQ=255)                                                                                                                                                                          ttttccagtagatGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGGTCAATATCAAT‑AGAAAACCACCTGAGCGGGCGACGGTGGTGGGGGTGCGG‑TGTTTGAAGGGCATTAAGCATAGAGCCATTGGTCCTAACACCCCCGACCCacac                                        >  2:11949/13‑251 (MQ=255)                                                                                                                                                                                   agcTGCGGGCGGCTGGCGACGTATGCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggtgaggt                               <  2:13596/249‑1 (MQ=255)                                                                                                                                                                                                  gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAAT‑AGAAACCCCCTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCCTTTACGAGAGCGCCATTGTGCATATCACCCTCGACCCCCCCCCGTGAGGGGATAAACCGCTCCAt                >  2:119944/1‑251 (MQ=255)                                                                                                                                                                                                     aCGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAg             <  2:168592/251‑1 (MQ=255)                                                                                                                                                                                                                 gcaaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTg  <  2:99527/247‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            GCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTAT‑AGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGG‑TTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTG  >  NC_000913/64992‑65449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑