Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,933 G→A I199I (ATC→ATT leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,9330GA97.1% 106.6 / ‑6.3 34I199I (ATC→ATTleuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (19/14);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATC  >  NC_000913/82704‑83176
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
tCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGGGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGttt                                                                                                                                                                                                                                >  1:67588/1‑250 (MQ=255)
 cgggCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTGCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCGTTGTCGATGt                                                                                                                                                                                                                                  <  2:204173/246‑1 (MQ=255)
 cTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGGATATCCTTACGAACTTTGATCGCCATGATGCCTTCTTCCAGGGGACAGTTTCCGGCACGCCCGCCGATCCCGTTCATTTCGCCTTCCACCTGGCGTGCACCGGCATGTACCCCCGCCAGTGAGGTGCCGACCGCCCGGCCTTAATCGTCGTGGGGATGTTCGGAAATAATGGCTTTGTCGATGt                                                                                                                                                                                                                                  >  1:204173/1‑248 (MQ=255)
                gctggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACa                                                                                                                                                                                                                <  1:49685/251‑1 (MQ=255)
                          cATATCTCCTGGTGATTAATGGCGGTGTGGACCTTGAGAATATCCTTCCGAACTTTGATAGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGGGGTGCCGCCCGCCAGGCCTTAATCGTTGCGGGGATGTACGGGAATAATGGCTTTTTCGATGTTAGGGACGCGGTCCTAAAGGCCGCtggt                                                                                                                                                                                                      >  1:109028/1‑249 (MQ=255)
                                   tGGTGATTAATGGCGGTGTGGACGTTGAGAAAATCCTTACGAAATTTGATAGCCATGATGACATTTTCCAGGGAACAGTTTCCGGCACGCCCCCCGATACCCCTCATTGCGCCTTCCACCTCGCGTGCAACGGCATGTACCGCCGCCAATGTGGTGCCCACCACCAGGCCTCAATAGTCGTGGGGATGTACGGAAAAAATGGCTTTTTCGCGGTTATGCACGCGTTCATAccgaccccggagtagtcccgc                                                                                                                                                                                             >  2:276413/1‑231 (MQ=255)
                                            aTGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCaaa                                                                                                                                                                                    <  1:145240/251‑1 (MQ=255)
                                                           ttGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGcc                                                                                                                                                                     >  2:287731/1‑251 (MQ=255)
                                                                 aaatcattACGAACTTTGATCGCCCTGATGACTTCTTCCAGGGAACAGTTTACGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCTAAATCGTCGTGGGTATACACGGAAATAAGGGCTTTGTCGATCTTAGGTACGCGTTCATACAGGCCGATGGTGACTCGGGCGAACTCAAACGGCAtctcgtcgccaccggt                                                                                                                                                               >  2:5609/7‑236 (MQ=255)
                                                                         aCGAACGTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTGAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGa                                                                                                                                                        <  2:185873/250‑1 (MQ=255)
                                                                            aaCTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCTGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGt                                                                                                                                                    >  2:217805/1‑251 (MQ=255)
                                                                               tttGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGCATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGTTg                                                                                                                                                  >  2:70304/1‑250 (MQ=255)
                                                                                  gATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtgg                                                                                                                                              >  1:291748/1‑251 (MQ=255)
                                                                                  gATCACCATGATGACTTCTTCCATGCAACTGTTTCCCGCACTCTCGCCGGTCCCTTTCATGTCGCCTTCCCCCTGGCGTCCACCGGCATGTACCGCATCCAGTGAGTTGGCGACCGCCAGGCCTAAATCGTCTTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCACACGGCATGGTGTAGACCACGGTTCCCGGAAGGTTGAtt                                                                                                                                               <  2:128046/250‑2 (MQ=255)
                                                                                           gatgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCAc                                                                                                                                     >  1:85464/1‑251 (MQ=255)
                                                                                                     tcCAGGGAACAGTTCCCGGCACGCTCGCCGATCCCGTTCATGGCGCCTTCCACCGGGCGTGCCCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTaa                                                                                                                            <  2:27348/250‑1 (MQ=255)
                                                                                                       cAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATcg                                                                                                                         >  1:305169/1‑251 (MQ=255)
                                                                                                              cAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTc                                                                                                                  >  2:51021/1‑251 (MQ=255)
                                                                                                               aGTTTACGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGGACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGCACTCAAACGGCATGGTGTAGCCCACCGTGTCCGGAATGTTGATGGGGGTGGCACCGGCATTAATCGCCGCTTCg                                                                                                                 >  2:259931/1‑251 (MQ=255)
                                                                                                                         aCGCTCGCCGATCCCGCTCATTGCGCCTTCCACCTGCCGTGCACCGGCCTGTACCGCCGCCAGTGAGTTTCCGACCGCCCGGCCCCAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAACCGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCCTTAATCGCCGCTTCGACCACTcgcg                                                                                                       >  2:311861/1‑251 (MQ=255)
                                                                                                                               gCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGAt                                                                                                 <  1:282785/251‑1 (MQ=255)
                                                                                                                                 cgagcccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCg                                                                                               <  2:200837/247‑1 (MQ=255)
                                                                                                                                     cccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCa                                                                                            >  1:5687/1‑250 (MQ=255)
                                                                                                                                                     tCCACCTGGCGTGCACCGGCATTTACCGCCGCCAGTGAGTTGCCGACCGCCAGGTCTAATTCGTCGTGGGTATGTGCGGAAAGAATGGCTTTGTCGATGTTAGGTACGCTTTCATACAGGCCGCTGAGGATTCCGGCGAACTCAAGCGGCATGGTGTAGCCCACGGTGTCCGGAATGTTTATGGTGGTGGCGCCGGCATTAATTGCCGCTTCGACCACTCGCGGCAGATCGGCAATGGGTGTACGCCCg                                                                             <  1:291009/249‑1 (MQ=255)
                                                                                                                                                       cACCTGGCGTGCCCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCAt                                                                         <  2:18635/251‑1 (MQ=255)
                                                                                                                                                                                 ccgccgGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCg                                               <  2:82020/251‑1 (MQ=255)
                                                                                                                                                                                    ccAGTGAGTTGCCGACCGCCAGGCCTAAAGCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGgtg                                            <  2:109028/251‑1 (MQ=255)
                                                                                                                                                                                              gccGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTGAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTTGCATCGGCAGTAATCGCCGCTTCGAGCACGCGCTCCAGAGCGGCAATGGGTGTTCGCCCGGCATCTTCGCAATAAATATCAACATCATGGGTGTAATGACggg                                  <  2:290766/250‑1 (MQ=255)
                                                                                                                                                                                                             taaaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCatat                   >  2:304156/2‑251 (MQ=255)
                                                                                                                                                                                                              aaaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCatat                   >  1:149912/1‑250 (MQ=255)
                                                                                                                                                                                                               aaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATatag                 <  1:320737/251‑1 (MQ=255)
                                                                                                                                                                                                                aTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCatat                   >  1:131647/1‑248 (MQ=255)
                                                                                                                                                                                                                          gTATGTAAGGAAATACTGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTc      >  2:117736/1‑251 (MQ=255)
                                                                                                                                                                                                                              gTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATc  >  2:68063/1‑251 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
TCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATC  >  NC_000913/82704‑83176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: