Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,894 T→G G212G (GGA→GGC leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,8940TG93.5% 78.8 / ‑2.7 31G212G (GGA→GGCleuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (15/14);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

GCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTG  >  NC_000913/82660‑83134
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
gcTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGGGGGCGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGcct                                                                                                                                                                                                                                   >  1:18635/1‑249 (MQ=255)
     caacaaTGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGTCCTAAAtcg                                                                                                                                                                                                                             <  1:230051/251‑1 (MQ=255)
         aaTGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCCGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTTCGCCTTCCACCCGGCGTGCACCGGCCTGTACCGCCCCCCGTGAGTTGCCGACCGCCCGGCCTAAATcgtcgt                                                                                                                                                                                                                         >  2:145240/1‑251 (MQ=255)
                 tttttgCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATg                                                                                                                                                                                                                  <  2:65576/248‑1 (MQ=255)
                                            tCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGGGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGttt                                                                                                                                                                                      >  1:67588/1‑250 (MQ=255)
                                             cgggCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTGCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCGTTGTCGATGt                                                                                                                                                                                        <  2:204173/246‑1 (MQ=255)
                                             cTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGGATATCCTTACGAACTTTGATCGCCATGATGCCTTCTTCCAGGGGACAGTTTCCGGCACGCCCGCCGATCCCGTTCATTTCGCCTTCCACCTGGCGTGCACCGGCATGTACCCCCGCCAGTGAGGTGCCGACCGCCCGGCCTTAATCGTCGTGGGGATGTTCGGAAATAATGGCTTTGTCGATGt                                                                                                                                                                                        >  1:204173/1‑248 (MQ=255)
                                                            gctggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACa                                                                                                                                                                      <  1:49685/251‑1 (MQ=255)
                                                                      cATATCTCCTGGTGATTAATGGCGGTGTGGACCTTGAGAATATCCTTCCGAACTTTGATAGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGGGGTGCCGCCCGCCAGGCCTTAATCGTTGCGGGGATGTACGGGAATAATGGCTTTTTCGATGTTAGGGACGCGGTCCTAAAGGCCGCtggt                                                                                                                                                            >  1:109028/1‑249 (MQ=255)
                                                                               tGGTGATTAATGGCGGTGTGGACGTTGAGAAAATCCTTACGAAATTTGATAGCCATGATGACATTTTCCAGGGAACAGTTTCCGGCACGCCCCCCGATACCCCTCATTGCGCCTTCCACCTCGCGTGCAACGGCATGTACCGCCGCCAATGTGGTGCCCACCACCAGGCCTCAATAGTCGTGGGGATGTACGGAAAAAATGGCTTTTTCGCGGTTATGCACGCGTTCATAccgaccccggagtagtcccgc                                                                                                                                                   >  2:276413/1‑231 (MQ=255)
                                                                                        aTGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCaaa                                                                                                                                          <  1:145240/251‑1 (MQ=255)
                                                                                                       ttGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGcc                                                                                                                           >  2:287731/1‑251 (MQ=255)
                                                                                                             aaatcattACGAACTTTGATCGCCCTGATGACTTCTTCCAGGGAACAGTTTACGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCTAAATCGTCGTGGGTATACACGGAAATAAGGGCTTTGTCGATCTTAGGTACGCGTTCATACAGGCCGATGGTGACTCGGGCGAACTCAAACGGCAtctcgtcgccaccggt                                                                                                                     >  2:5609/7‑236 (MQ=255)
                                                                                                                     aCGAACGTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTGAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGa                                                                                                              <  2:185873/250‑1 (MQ=255)
                                                                                                                        aaCTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCTGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGt                                                                                                          >  2:217805/1‑251 (MQ=255)
                                                                                                                           tttGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGCATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGTTg                                                                                                        >  2:70304/1‑250 (MQ=255)
                                                                                                                              gATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtgg                                                                                                    >  1:291748/1‑251 (MQ=255)
                                                                                                                              gATCACCATGATGACTTCTTCCATGCAACTGTTTCCCGCACTCTCGCCGGTCCCTTTCATGTCGCCTTCCCCCTGGCGTCCACCGGCATGTACCGCATCCAGTGAGTTGGCGACCGCCAGGCCTAAATCGTCTTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCACACGGCATGGTGTAGACCACGGTTCCCGGAAGGTTGAtt                                                                                                     <  2:128046/250‑2 (MQ=255)
                                                                                                                                       gatgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCAc                                                                                           >  1:85464/1‑251 (MQ=255)
                                                                                                                                                 tcCAGGGAACAGTTCCCGGCACGCTCGCCGATCCCGTTCATGGCGCCTTCCACCGGGCGTGCCCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTaa                                                                                  <  2:27348/250‑1 (MQ=255)
                                                                                                                                                   cAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATcg                                                                               >  1:305169/1‑251 (MQ=255)
                                                                                                                                                          cAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTc                                                                        >  2:51021/1‑251 (MQ=255)
                                                                                                                                                           aGTTTACGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGGACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGCACTCAAACGGCATGGTGTAGCCCACCGTGTCCGGAATGTTGATGGGGGTGGCACCGGCATTAATCGCCGCTTCg                                                                       >  2:259931/1‑251 (MQ=255)
                                                                                                                                                                     aCGCTCGCCGATCCCGCTCATTGCGCCTTCCACCTGCCGTGCACCGGCCTGTACCGCCGCCAGTGAGTTTCCGACCGCCCGGCCCCAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAACCGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCCTTAATCGCCGCTTCGACCACTcgcg                                                             >  2:311861/1‑251 (MQ=255)
                                                                                                                                                                           gCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGAt                                                       <  1:282785/251‑1 (MQ=255)
                                                                                                                                                                             cgagcccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCg                                                     <  2:200837/247‑1 (MQ=255)
                                                                                                                                                                                 cccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCa                                                  >  1:5687/1‑250 (MQ=255)
                                                                                                                                                                                                 tCCACCTGGCGTGCACCGGCATTTACCGCCGCCAGTGAGTTGCCGACCGCCAGGTCTAATTCGTCGTGGGTATGTGCGGAAAGAATGGCTTTGTCGATGTTAGGTACGCTTTCATACAGGCCGCTGAGGATTCCGGCGAACTCAAGCGGCATGGTGTAGCCCACGGTGTCCGGAATGTTTATGGTGGTGGCGCCGGCATTAATTGCCGCTTCGACCACTCGCGGCAGATCGGCAATGGGTGTACGCCCg                                   <  1:291009/249‑1 (MQ=255)
                                                                                                                                                                                                   cACCTGGCGTGCCCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCAt                               <  2:18635/251‑1 (MQ=255)
                                                                                                                                                                                                                             ccgccgGTGAGTTGCCGACCGCCAGGCCTAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCg     <  2:82020/251‑1 (MQ=255)
                                                                                                                                                                                                                                ccAGTGAGTTGCCGACCGCCAGGCCTAAAGCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGgtg  <  2:109028/251‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
GCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTG  >  NC_000913/82660‑83134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: