Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,633	T→G	I299I (ATA→ATC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,633	0	T	G	100.0%	94.2 / NA	29	I299I (ATA→ATC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/8);  total (21/8)

AGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCAT  >  NC_000913/82397‑82874                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   aGCGCCTCAAGATCGTAATCAGACCCCTGACCTTTTTTGTCCGCCAGCTTCAGGCAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAgtg                                                                                                                                                                                                                                      >  2:295832/1‑251 (MQ=255)  gcgcCGCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAgtgt                                                                                                                                                                                                                                     <  1:225030/251‑1 (MQ=255)   cgcCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGtgtg                                                                                                                                                                                                                                    >  2:49685/1‑251 (MQ=255)        ccAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTGCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACGTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAAt                                                                                                                                                                                                                               <  2:60040/251‑1 (MQ=255)                    cAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATGCACTTTCTTTATACCCCATCTCATCCATGCGATGTTGCACCGCCGCACGCCCCGAACGAGATGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGGGGATACCGGAGGAGTGTGCGAATGCGCCGCTGcc                                                                                                                                                                                                                    <  1:295832/250‑1 (MQ=255)                                                          gCATCGTACAAATTGTCTAAATTATATTCACTTTATTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGATCAGATTCAGCTGGATTTCGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGAACGCCATCCTGGTGGATACCGGAGGAGAGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAAt                                                                                                                                                                             >  2:57160/1‑251 (MQ=255)                                                                       tGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCtaactaac                                                                                                                                                                >  1:82020/1‑251 (MQ=255)                                                                        gTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGACCCGAACGAGAGGTCAGATTCAGCTGGCTTTGGCTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGACTGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTAGCTaactaact                                                                                                                                                               >  1:290766/1‑251 (MQ=255)                                                                                                          tcATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGGGCGCCATATCTCCTGGTGATTAATGGc                                                                                                                             >  2:22138/1‑251 (MQ=255)                                                                                                           cATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCg                                                                                                                            >  1:238444/1‑251 (MQ=255)                                                                                                                     aTGTTGCACCGCCGCACGCCACGAACGAGAGGTCAGATTCAGCTGGATTGTGTTCAGACCAAGAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCGGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCGAACAATGTCGTTGTTTGCCGGGATCGGCATAGTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCGGGTGATTAACGGTGGTGTGGACGt                                                                                                                  <  2:196818/251‑1 (MQ=255)                                                                                                                           cACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTgaga                                                                                                             >  1:211632/1‑250 (MQ=255)                                                                                                                                ccgcACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTGAGaaaaacc                                                                                                       >  1:266881/1‑246 (MQ=255)                                                                                                                                ccgcACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATcc                                                                                                       >  1:53661/1‑251 (MQ=255)                                                                                                                                           gAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACttt                                                                                            >  1:147681/1‑251 (MQ=255)                                                                                                                                                       aGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTTGGAAATTCCTTACGCACTTTGATACCCatgat                                                                                >  2:243415/1‑251 (MQ=255)                                                                                                                                                           tCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGAc                                                                             <  1:225192/250‑1 (MQ=255)                                                                                                                                                            cAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCCTGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGGTGCGAATATCCTTTCGAACTTTTATCGCCAAGATGActt                                                                           >  1:292638/1‑251 (MQ=255)                                                                                                                                                                        gTTCAGACCAATAGCTTCTGGTGTCATGATTTCGTCGTTTTCGCGGTCTTTCAGCCCGCCATACTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGCGATTAATGGCGGTGTGGAAGCTGAGAATCTCACTACGAAACTTGATCGCCCTTATTGCTT‑CTTCCCGGGAAc                                                              >  2:35314/1‑251 (MQ=255)                                                                                                                                                                         ttCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATTCTTTCGAACTTTGATTGGCCTGATGCCTT‑TTTCCAGGGAAcc                                                             >  1:200837/1‑250 (MQ=255)                                                                                                                                                                            aGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGtt                                                          <  1:188824/251‑1 (MQ=255)                                                                                                                                                                              aCCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGTTTc                                                        <  2:53661/251‑1 (MQ=255)                                                                                                                                                                               ccAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGGGGGGACGTTGCGAATATCCTTACGGACTTTGATCGCCCTGATGGCTTTCTTCC‑GGGAAAAGTTTcc                                                       >  2:134564/1‑251 (MQ=255)                                                                                                                                                                                   tAGATTCTGGTGTCATGATTTCGTCGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATTCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGTTTCCGGc                                                    >  1:261354/1‑250 (MQ=255)                                                                                                                                                                                           ggTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATAGGCATATTACAAATCTGGCTAACTAAATGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGCGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTTCAGGGGACAGGTTCCGGCACGcctgcc                                           >  1:41511/1‑246 (MQ=255)                                                                                                                                                                                                      ttCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGGTTCCGGCACGCTCGCCGATCCCGTTc                                 >  1:185873/1‑250 (MQ=255)                                                                                                                                                                                                                 cgcgGTTTTTCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAACCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAAAAGTTTCCGGCACGCTCGCCGGTCCCGTTCATTGCGCCTTcc                     >  1:136198/1‑251 (MQ=255)                                                                                                                                                                                                                        tttCAGCACGCCATCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTgg               <  2:41511/250‑1 (MQ=255)                                                                                                                                                                                                                                    aTCCTGGTGGATACCGGAGGAGTGTGCGAATGCGCCGCTGCAAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGGTGAGAATATACTTACGAACTTTGATCGCCATGATGACTT‑CTTCCCGGGAAAAGTTTCCGGCACGCCCGCCGATCCCGTTCATTGCGCCCTCCACCTGGCGTGCACCGGCAt  >  1:19278/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   AGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTT‑CTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCAT  >  NC_000913/82397‑82874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑