Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,529	C→G	G112G (GGG→GGC)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,529	0	C	G	100.0%	102.8 / NA	30	G112G (GGG→GGC)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/18);  total (12/18)

CGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGG  >  NC_000913/80291‑80775                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       cGGTTTTACCGATAATTGCCAGCACGCTATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTCCGTgg                                                                                                                                                                                                                                            >  2:302428/1‑251 (MQ=255)                 tGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATAc                                                                                                                                                                                                                             <  1:285592/250‑1 (MQ=255)                       cACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGAt                                                                                                                                                                                                                      <  1:231205/251‑1 (MQ=255)                         cGATATCGTTTGCTGTAATGCCCGGCGCGGCTTTGCCCTGGACTGCAAGTTTCATGGTTTTTGCGCGGTTCTGTTTCAGGGGTGGCGTTGCCAGTACGTGTTCAACGTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGCGACGCACTGTTCCGGGCCCATGACGTGGACGATCCCCTGGTACGGGTGATTc                                                                                                                                                                                                                    <  2:256297/251‑1 (MQ=255)                                           ggCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACg                                                                                                                                                                                                  <  2:220808/250‑1 (MQ=255)                                            gCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCAAGTACGCGTTCAACTTCGGAAGTGCCGCTAACAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCCAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGc                                                                                                                                                                                                 >  1:201076/1‑251 (MQ=255)                                                ggCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCaaa                                                                                                                                                                                             <  1:158733/251‑1 (MQ=255)                                                     ggCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCtt                                                                                                                                                                                        >  1:113525/1‑251 (MQ=255)                                                      gCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCttt                                                                                                                                                                                       <  2:30895/251‑1 (MQ=255)                                                           gCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAg                                                                                                                                                                                   <  1:244602/250‑1 (MQ=255)                                                                ggACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGttttt                                                                                                                                                                              <  1:53775/250‑1 (MQ=255)                                                                      cAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTTCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAg                                                                                                                                                                        <  1:317196/250‑1 (MQ=255)                                                                       aaTTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGtt                                                                                                                                                                      <  2:66772/251‑1 (MQ=255)                                                                                 gTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTg                                                                                                                                                            >  2:35368/1‑251 (MQ=255)                                                                                      tGCGCGGCCCGGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATa                                                                                                                                                        <  1:280429/250‑1 (MQ=255)                                                                                          cgGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACgcgc                                                                                                                                                   >  2:23962/1‑251 (MQ=255)                                                                                                                  gCCAGTACGTGTGCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATGTCACCGCAGGCATTAATGTc                                                                                                                           <  2:309470/251‑1 (MQ=255)                                                                                                                     aGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCttt                                                                                                                        <  2:271696/251‑1 (MQ=255)                                                                                                                         cGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTTATCAGTTCCTGCAGCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                                                                                                                    <  1:24407/251‑1 (MQ=255)                                                                                                                            gtTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTgg                                                                                                                 <  1:178715/251‑1 (MQ=255)                                                                                                                                            gtcGATACCAAAGGCCAGTGCGCCAAACGCTCCGTGGGTGGCGGTATGCGAATCGCCACAGACAATGGTCATCCCCGGCCAGGTGTCACCCTGTTCCTGGCCCATTACGTGGACGATCTCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTATTTCCAGGTTTTGATCAGTTCCTGCATCTGGATACGGGCCATTTCACCGCAGGCATTACTGTCTTTTGTCTGGGTagttcagttgtgat                                                                                                 <  2:72556/249‑13 (MQ=255)                                                                                                                                                  aCCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTACGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATgg                                                                                           >  2:37899/1‑251 (MQ=255)                                                                                                                                                    cAAAGGCCAGTGCGCCAAACGCGCCGTGGGGGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGATGACGCCCTATTCCGGGCCCATTACGTGGACGATCCCCCACTACGGGTACTTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTACATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTCACGTCTTTGACCAACACAGAGACGTTGTGATCCATGGt                                                                                          >  2:244954/1‑250 (MQ=255)                                                                                                                                                           cAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCACCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAgg                                                                                  >  2:22878/1‑251 (MQ=255)                                                                                                                                                                    aaaCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCCGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCg                                                                         >  1:106412/1‑251 (MQ=255)                                                                                                                                                                                             gAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgc                                                >  2:234472/1‑251 (MQ=255)                                                                                                                                                                                             gAGTCGCCGCAGACAAGGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGGGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTGTTGATCAGTTCCTGCATCTGGAGACGCGCCATTTCACCGCAGGCATTAATGTCGTTGGTCTGGGTAGAGACGGTGGGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgc                                                <  1:15503/251‑1 (MQ=255)                                                                                                                                                                                                     gcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGAc                                        >  2:276934/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTTCCGGGCCCATTACGTGGACGATCCCCCGATACGGGTGATTCAGGTCATACAGTTAGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCAcc     >  2:141233/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAgg  <  1:10357/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       CGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGG  >  NC_000913/80291‑80775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑