Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,040	A→G	A138A (GCT→GCC)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,040	0	A	G	100.0%	72.4 / NA	24	A138A (GCT→GCC)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/6);  total (18/6)

TGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTT  >  NC_000913/78800‑79283                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    tgaCGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTACGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCAcc                                                                                                                                                                                                                                            >  1:239446/1‑250 (MQ=255)     gaggACGATAGCGGAAAGCCCGGTCATTTGAACGGGCAAGGGGATTAAATCCTAACCGCAGGTTGATTAGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCACAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATTCCCGGATTGGCTTTCACCAgcgc                                                                                                                                                                                                                                       >  1:26705/3‑251 (MQ=255)                     aGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACt                                                                                                                                                                                                                       <  2:5779/251‑1 (MQ=255)                               tttGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGc                                                                                                                                                                                                             >  1:46002/1‑251 (MQ=255)                                       ggCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTc                                                                                                                                                                                                     <  2:265982/251‑1 (MQ=255)                                                     ttCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTg                                                                                                                                                                                       >  1:219485/1‑251 (MQ=255)                                                          aaaCGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGgttgt                                                                                                                                                                                  >  1:24582/1‑251 (MQ=255)                                                                     gttTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGCTCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTg                                                                                                                                                                       >  2:245472/1‑251 (MQ=255)                                                                                    cGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGTCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCCGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTc                                                                                                                                                         >  2:239723/1‑250 (MQ=255)                                                                                       cAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGc                                                                                                                                                      >  2:259844/1‑250 (MQ=255)                                                                                             cgtcgtcgtGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTc                                                                                                                                               >  2:34915/1‑251 (MQ=255)                                                                                              gtcgtcgtGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCCGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCCGGTTGTTAAAGCTATTGCCCGAGAAGATGTCCGCAAAACTcc                                                                                                                                              >  2:88524/1‑250 (MQ=255)                                                                                              gtcgtcgtGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAAcccg                                                                                                                                              >  2:88540/1‑248 (MQ=255)                                                                                                   cgtGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCaaacgtctttaac                                                                                                                                         <  2:240915/251‑11 (MQ=255)                                                                                                                              ccAGACCGTTCATCATGCAGTGGCGGCGTAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAATCCGTAGTCGGTc                                                                                                              <  2:305314/251‑1 (MQ=255)                                                                                                                                                               cATCGATGGTAAAGCGATAGGTTTTCTCTCCAGCTTTAACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTCCACCGGCAGAAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGcc                                                                              >  2:135575/1‑250 (MQ=255)                                                                                                                                                                aTCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCcac                                                                            >  1:208422/1‑251 (MQ=255)                                                                                                                                                                              gATAGGTTTTCTCTAGCGCTTAAACATCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGACTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCAACGGCAGCAGCTGGCTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGAAGTCGGTCAAGGCCCAGGGCGCGTGCTCACGCGACGAGCCACAGCCGAAGTTTtct                                                              >  2:207618/1‑251 (MQ=255)                                                                                                                                                                                 aGGTTATCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGCTCCCCGGAGTGGCTTTCACCAGCGCAAACAGTTCGTCAACTTCTGCATTGCTTAATTTCACCTGCAGCAGCTGTTTGTTAAAGCTATTGCCGTTGAAGATGTCAGCTAAACTCGGCGCAATCCCCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTGTCTCGt                                                           <  2:282091/251‑1 (MQ=255)                                                                                                                                                                                                        tctTGCACTTACAGAGACACGTCGAAACGGAACCACGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCAATTCTGCATCGCTTAATTTCACCGGCAGCCGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGATCAATGCCCAGGGCGCGTGCTCACGAGAAGAGCCACAGCCGAAGTTTTATCGTGCCAGCGAAATGGACGCGACCTg                                    >  2:245565/1‑251 (MQ=255)                                                                                                                                                                                                          ttGCGCTCCAAGATCCAAGTCGAAAGGGATCCCAGTATTGGGTTTCACCCGCGCTACCCGTTTGTCAACTTCTGAATCGCTTCATTTCACCGGCAGCAGCTGGCTGTCAAAGCTATTGACGTAGAAGTTGTCAGCAACACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCACGGGCGCGCGCTAACGCCAAGAGCTACCGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGAt                                  >  2:42436/1‑251 (MQ=255)                                                                                                                                                                                                                    aGATCCACGTCGAAATGGATCCCCGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAACCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGa                         >  2:153133/1‑250 (MQ=255)                                                                                                                                                                                                                        ccACGACGAAATGGATCACCGGATTGGCTTTCCCCAGCGCCAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCCGCTGGTTGTTAAAGCTCTTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGCAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATCCTGCGGGAAGTTc                    >  2:185736/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGGATTGGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGttt  <  1:184211/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    TGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTCTTGCGCTTCCAGATCCACGTCGAAATGGATCCCCGGATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTT  >  NC_000913/78800‑79283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑