Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,761	G→A	V381I (GTC→ATC)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,761	0	G	A	100.0%	40.2 / NA	13	V381I (GTC→ATC)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

GTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTC  >  NC_000913/78538‑79000                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                gTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTa                                                                                                                                                                                                                      <  1:95875/251‑1 (MQ=255)     atgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTGGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGa                                                                                                                                                                                                                  <  2:155837/251‑1 (MQ=255)                   ttttAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATGTCACAAAGCTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCACAGGTTAAAGACGTTTGATGACGTg                                                                                                                                                                                                    >  2:85251/1‑251 (MQ=255)                          gCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATa                                                                                                                                                                                             <  2:96919/251‑1 (MQ=255)                                         aTTGTTGCGGGTATTGGGATGCTATGGTTTCAGGAGTTAATGACTGGAAGAGCGGTGGCAGCTACCACCTTATTTACTATCATTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATGGCGGTTATTTCTGTTATCGCATGATTTGTAACCGCAAAGGTTAAAGACGTTTGATGACTGGGACGATAGCGGatcgccctgtc                                                                                                                                                                              <  2:270027/251‑11 (MQ=255)                                                           aTGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAgggg                                                                                                                                                            <  1:16191/251‑1 (MQ=255)                                                                 tGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                                                                                      >  1:2535/1‑251 (MQ=255)                                                                                atgcatatcagcgCGGGGGCAGATACCCCCTTACTTACTCACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATCCAGGGAGCGATTGCACACAGTTGGGGGCGCTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCACAGGTTAAAGACGTTTGATGACGTGAACGATAACGGAAAGACCGGACATTTGACCGGGCACGGGGATTAATTCATAAACACAGgtt                                                                                                                                       >  2:202770/13‑251 (MQ=255)                                                                                                                   tACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtg                                                                                                     <  2:153901/250‑1 (MQ=255)                                                                                                                                         gTAATTCTGGCTGGCGTTATTCAGGGAGCACTTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTACTTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGt                                                                              >  2:125594/1‑251 (MQ=255)                                                                                                                                                                   aGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTggcgg                                                    >  1:290652/1‑251 (MQ=255)                                                                                                                                                                                                 tGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGAAGTGGACGATAGCGGAAAGCACGGTAATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCAGCGGACGGCAACGATGGGCAAGCGATAGGtt                      >  2:285784/1‑251 (MQ=255)                                                                                                                                                                                                                      tATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACctc  <  1:309246/250‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                GTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGGTTTTCTCTCCCGCTTTCACCTC  >  NC_000913/78538‑79000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑