Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,767	C→T	A511A (GCG→GCA)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,767	0	C	T	100.0%	132.5 / NA	38	A511A (GCG→GCA)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

TCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑T‑GGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACA‑‑GAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATT  >  NC_000913/75534‑75998                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          tCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGAcc                                                                                                                                                                                                                            <  2:49260/249‑1 (MQ=255)           tATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGATGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTTGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCa                                                                                                                                                                                                                 <  1:187257/250‑1 (MQ=255)            aTCCGGATCAGGCTCGACGGGCATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGCGTAGTGCTTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTACCATCGAACCAGCCGAGGGGATTCCTGCGTAGGCCGCGCCTACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGCTGCTGGCAcc                                                                                                                                                                                                               >  2:309588/1‑251 (MQ=255)                 caTCACGTTCGACGTGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTAAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAA‑TCGGCACCAATTCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc                                                                                                                                                                                                          <  2:37404/250‑1 (MQ=255)                    cAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGcc                                                                                                                                                                                                       >  2:183670/1‑251 (MQ=255)                                   ttttCTCAGCGCCCGCGTACGCTTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCACCAATCATGGATCCGGTGGCTCAAACCACGCTGATTTAAAATCGACCCAGCCGAGGGTATTCATGCGTAGGCCGCGCTTACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCTCCAGTTCGCCAGGTTCATTTCGcc                                                                                                                                                                                         <  2:239882/250‑1 (MQ=255)                                   ttttCCCAGCGCACGAGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGACTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCAGCGCATAATGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGCTGCTGGCAGCAGTTCGCCAGGTTCATTTCGcc                                                                                                                                                                                         >  2:269343/1‑250 (MQ=255)                                     ttCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCAt                                                                                                                                                                                       <  1:119263/250‑1 (MQ=255)                                                  cgtacgcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTCTTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCgggcg                                                                                                                                                                         >  1:29321/1‑247 (MQ=255)                                                       gcgttgctacccGTTGAGAACGGCGGGAGTGGAGTGATTTTGTTATCAACCAGCAATCATGGAGCCGTTGGCGCAAACCACTCTGATTTAAAATCTAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGTCCCTGAATGATCAGCCATTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTc                                                                                                                                                                    <  2:165380/242‑1 (MQ=255)                                                                  cGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGt                                                                                                                                                         >  1:232164/1‑251 (MQ=255)                                                                                    tgtagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGGGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGtgtt                                                                                                                                       >  2:162704/1‑251 (MQ=255)                                                                                    tgtagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGtgtt                                                                                                                                       >  2:78875/1‑251 (MQ=255)                                                                                            ttttGTTATCACCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAACATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTcgatcggcatacagtgttggagcggc                                                                                                                               >  2:289549/1‑226 (MQ=255)                                                                                              ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAgcg                                                                                                                              <  2:251152/250‑3 (MQ=255)                                                                                                          aGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCaca                                                                                                                  >  2:297754/1‑248 (MQ=255)                                                                                                                              cgcAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaat                                                                                             <  1:56747/251‑12 (MQ=255)                                                                                                                                          tGATTTAAAATCGCACCAGCCGAGGGTCTTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGAAAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAgcg                                                                               >  1:94868/1‑249 (MQ=255)                                                                                                                                          tGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAgcg                                                                               >  2:209849/1‑249 (MQ=255)                                                                                                                                           gATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAgcgt                                                                              <  1:21077/251‑4 (MQ=255)                                                                                                                                           gATTTAAAATCGAACCAGCCGAGGGTATTCATGCGGAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatccagctgcagc                                                                                  <  2:293309/249‑23 (MQ=255)                                                                                                                                                     cGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGgc                                                                     <  1:155581/250‑1 (MQ=255)                                                                                                                                                       aaCCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GACACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCt                                                                  >  2:160708/1‑251 (MQ=255)                                                                                                                                                           aGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGtt                                                               >  2:78359/1‑250 (MQ=255)                                                                                                                                                                  ggTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGcc                                                          >  1:80356/1‑248 (MQ=255)                                                                                                                                                                          tGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGCATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATcgctc                                               >  2:148301/1‑247 (MQ=255)                                                                                                                                                                             gtaGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCACGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATcgctctcg                                            <  1:198389/249‑8 (MQ=255)                                                                                                                                                                                gtccGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGAtc                                         <  1:264216/249‑1 (MQ=255)                                                                                                                                                                                         tACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GCAAAATCCAGCGGCAGCGTACAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttc                                 >  2:94937/1‑248 (MQ=255)                                                                                                                                                                                           cTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttcatg                              >  1:174253/1‑246 (MQ=255)                                                                                                                                                                                             gCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttcatgc                             <  1:297754/250‑7 (MQ=255)                                                                                                                                                                                               gTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGACCAGC‑‑GAAAAATCCAGCGTCAGCGTAAAGTTCGAGCTGTGCAGACAGATATCGCTCTCGATCTATCCttcatgtcac                          >  2:12568/1‑242 (MQ=255)                                                                                                                                                                                                 ggCCCCGGAATGATCAGCCAGTGGTG‑GATAAGC‑GGCACAATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACt                         <  2:174253/249‑1 (MQ=255)                                                                                                                                                                                                        gAATGATAAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTGCGCCAGGTTCATTTCGCCACTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAA‑‑GTGCGAGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGACAGATATCGCTCTCGATCTCTACTTCATGCCACTGATCGTa                  <  2:165493/250‑1 (MQ=255)                                                                                                                                                                                                             aTCAGCCAGTGGTG‑GATAAGC‑GGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAgctgat            >  1:277000/1‑246 (MQ=255)                                                                                                                                                                                                                      agtggtggataagcggCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCGCTCCTTCAGGCCACTGATCGGAGCTGATCTCTTTg     <  2:29321/237‑1 (MQ=255)                                                                                                                                                                                                                        tggtggataagcggCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAt   >  1:113146/13‑251 (MQ=255)                                                                                                                                                                                                                         ggtggataagcggCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGC‑‑GAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAtt  >  1:152724/12‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          TCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑T‑GGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACA‑‑GAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATT  >  NC_000913/75534‑75998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑