Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	74,308	T→C	I72V (ATA→GTA)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	74,308	0	T	C	100.0%	144.7 / NA	43	I72V (ATA→GTA)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGG‑ATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAC  >  NC_000913/74073‑74553                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ttaaaacatacgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGGACGACAGAGAGCAgcgc                                                                                                                                                                                                                                        >  2:71441/16‑248 (MQ=255)  tAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGcc                                                                                                                                                                                                                                       >  1:173854/1‑251 (MQ=255)   aaaaaaTACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCg                                                                                                                                                                                                                                      <  1:20999/251‑1 (MQ=255)     aaaaatgttgcgCCAGCAAAAGTCCTAGCAGCCCGTAGGGCGAAAAGAGCCACACCAGCCCGAGCTATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCTCAGACGCGACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAAGATCGCGGGTACGACAGAGATCAGCGCCgag                                                                                                                                                                                                                                    <  2:191662/240‑1 (MQ=255)        atacgcgcgCCAGCAAAATCCCTTGCACGACGTAGGGCGCAAAGGTCCACACCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCCTAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGCGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGGACGACAGATAGCAGCGCCgcg                                                                                                                                                                                                                                    >  2:135240/9‑246 (MQ=255)          acgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGaaa                                                                                                                                                                                                                               <  1:202371/245‑1 (MQ=255)           cgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAAc                                                                                                                                                                                                                              <  2:150944/246‑1 (MQ=255)            gtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACg                                                                                                                                                                                                                             <  1:2646/247‑1 (MQ=255)                 ccAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGc                                                                                                                                                                                                                        >  1:207322/1‑251 (MQ=255)                 ccAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGc                                                                                                                                                                                                                        >  1:161175/1‑251 (MQ=255)                       aaaTACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGa                                                                                                                                                                                                                    >  1:130772/1‑249 (MQ=255)                        aagaCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAgg                                                                                                                                                                                                                 <  2:198416/248‑1 (MQ=255)                           aCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAgg                                                                                                                                                                                                                 >  2:151470/1‑248 (MQ=255)                            ccTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGgaga                                                                                                                                                                                                              >  1:37861/1‑250 (MQ=255)                                         aGGGCGAAAAGGGCCACGCCCGACCGGGCGATTGGCAGAGTGTGGCCAGCCAGCCCTGGCGAACATAGACGCTAAGGATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGCAGTATAAGCGCACCACATGc                                                                                                                                                                                                <  2:185914/251‑1 (MQ=255)                                              gAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACTCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATGAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAg                                                                                                                                                                                           <  2:289323/251‑1 (MQ=255)                                                    gTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCt                                                                                                                                                                                     <  2:100498/251‑1 (MQ=255)                                                        aCTCCAGACCGAGCGATTGGCAGAGGGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGACGAAAACAGCAAACAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGGACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTcc                                                                                                                                                                                 >  1:283785/1‑251 (MQ=255)                                                              gACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAg                                                                                                                                                                           <  2:215367/251‑1 (MQ=255)                                                                       aTTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCAcc                                                                                                                                                                  <  2:261145/251‑1 (MQ=255)                                                                                  gtTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCt                                                                                                                                                        <  1:320314/250‑1 (MQ=255)                                                                                    tGCCAGCCAGCCCTGGCGACCATAGCCGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCCCAGACGCAGCAGCGCCAGCCGACCCGTAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTCCGACAGAGAGCAGCGCCGATAGAAACGCCGGCCAGAAGGAGAAGCGCGCCACATGCCACAGATAGCTGGCCTGCCAGACTGCCCCCCAGTCATCCTgc                                                                                                                                                      <  2:141369/250‑1 (MQ=255)                                                                                     gccagccgCCCCCGGCGACCGGAGACGCTAAGAATGCCGAAAACAGCATCCGGCACCGGGAGG‑ATTAAGGACGTTGCGCACAGCCGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGAGAGAGCGCGCGGGCGAGGAAGATCGCGGGTACGACAGAGAGCAGCGCGGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACAGCCACCCAGGCATCCTgcg                                                                                                                                                     <  2:47884/250‑1 (MQ=255)                                                                                         gccagccCTGGGGAACATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGcgcg                                                                                                                                                <  2:177129/251‑1 (MQ=255)                                                                                               ccTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTccac                                                                                                                                          <  1:100305/251‑1 (MQ=255)                                                                                                    cGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCcaccac                                                                                                                                       >  1:66413/1‑249 (MQ=255)                                                                                                                    gAATGCCGAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATGGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCGTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACgccgcc                                                                                                                     <  2:252097/251‑1 (MQ=255)                                                                                                                       ggccgaaaccagcacacagcacCGGGGGG‑ATTAAGGTCATTGCGCACAGCCGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAACAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCCCAGGGCGAGAAACGCCGCCAgc                                                                                                                  <  2:219136/232‑1 (MQ=255)                                                                                                                           gAAAACAGCAACCAGCACCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGCCAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCa                                                                                                               >  1:132333/1‑250 (MQ=255)                                                                                                                                       cagcacCGGGAGG‑ATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTaccaccc                                                                                                  >  2:25392/4‑250 (MQ=255)                                                                                                                                        cagcacGGGAGGGATTAAGGTCATTGGTCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCGGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGGCCTGCCATACTGCCACCCAGTCAGCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCAAGCGCAACCGCTAccacca                                                                                                  <  2:182573/246‑1 (MQ=255)                                                                                                                                         ccacCGGGAGG‑ATTAAGGTCAGTGCGCACAGACGCAACAGCGCCAGCCGACCCGAAAAGCGCCTGCGATAGGGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCTCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACAGTCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCTCCAGc                                                                                                <  2:45368/250‑1 (MQ=255)                                                                                                                                                gagg‑aTTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGtggtgg                                                                                         <  1:239759/251‑1 (MQ=255)                                                                                                                                                 agg‑aTTAAGGTCATTGCGCACAGACGCAACAGCTCCAGCCGACCCGGACAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGACCTGCCAGACTGCCACCCCGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGgc                                                                                        >  2:227045/1‑251 (MQ=255)                                                                                                                                                                       aGACGCAACAGCGCCAGACGACCCGGAAAGCGCCCGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTCCCACCAGCGCGGCGGCGCTTACACCTGGAATTAAcc                                                                    >  2:257300/1‑250 (MQ=255)                                                                                                                                                                         aCGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGc                                                                 >  1:77027/1‑251 (MQ=255)                                                                                                                                                                          cacAACAGCGCCAGCCGACCCGGAAAGCGCCTGCTATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCTCATGCCACATAGAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCTCCAGCGCAACCGCTACCATCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGcc                                                                <  1:180092/249‑1 (MQ=255)                                                                                                                                                                                      aGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACgg                                                    >  1:128679/1‑251 (MQ=255)                                                                                                                                                                                        ccGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCt                                                  >  1:218391/1‑251 (MQ=255)                                                                                                                                                                                         cGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTg                                                 >  1:240226/1‑251 (MQ=255)                                                                                                                                                                                                           cGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCAACCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCg                               <  2:222692/251‑1 (MQ=255)                                                                                                                                                                                                               agagCGCGCGGGCGAGGAATATCGCGAGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCCGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGCGGCGCTTACCCCTGGAATTAACAAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTa                            >  2:308131/1‑250 (MQ=255)                                                                                                                                                                                                                                        gggTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAc  >  2:62565/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      TTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGG‑ATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCACAGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAC  >  NC_000913/74073‑74553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑