Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,445	C→A	L359L (CTG→CTT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,445	0	C	A	100.0%	101.4 / NA	33	L359L (CTG→CTT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/5);  total (28/5)

ACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCC  >  NC_000913/73210‑73689                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTc                                                                                                                                                                                                                                       >  2:89886/1‑251 (MQ=255)                 ggTGATACCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGTCCGAAGTTCGCGACTGCTCCCTAGCAGCATCATgg                                                                                                                                                                                                                       >  2:251967/1‑251 (MQ=255)                                 gggTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTAAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATAATGGTAAGGACTACGcaca                                                                                                                                                                                                        >  1:135691/1‑250 (MQ=255)                                    ttttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATac                                                                                                                                                                                                    >  2:75874/1‑251 (MQ=255)                                      ttccattaCTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCAAAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCAGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGACGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAAAacac                                                                                                                                                                                                  >  1:317732/8‑251 (MQ=255)                                      ttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATacac                                                                                                                                                                                                  >  2:36838/1‑251 (MQ=255)                                               tttCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGACGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCCTTTTCTGCCGCGCCCGAAGTTCGCTACTGCTCCATAGCCGCATCATGGTCAGCACTACGCACAATCCACCTgccgccc                                                                                                                                                                                         >  2:282258/1‑250 (MQ=255)                                               tttCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTgccgcc                                                                                                                                                                                          >  1:291621/1‑250 (MQ=255)                                                  aaGCGCATAAGGGATCGCCATTAACGCATTGGTAAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTTTGGAGAAGAAAAAAAAAGCCGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCAGCATGGTCAGCACTACGCACAATACACCTGCCGCCAgc                                                                                                                                                                                       <  1:18887/249‑1 (MQ=255)                                                        aTAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATCCACCTGCCGCCAGCGCAATAc                                                                                                                                                                                >  2:254887/1‑251 (MQ=255)                                                        aTAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGCCAGCAGAATGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCAATACGCACAATACACCTGCCGCCAGCGCAATAc                                                                                                                                                                                >  1:292153/1‑251 (MQ=255)                                                               aTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACg                                                                                                                                                                          >  1:70510/1‑250 (MQ=255)                                                                     aTTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTcc                                                                                                                                                                    >  1:321302/1‑250 (MQ=255)                                                                        accgcttggttaaaaaTCACAATGCCGTCACAAGTTGGCGGCAGGCCAATGGTGTTGTTAAGCAGTAAAAAGAGGCCGGTAGCCACCCCAACCCCCGGCATGGCGAGGATCAACATGCCGCTCACCCCCAGCGCCTAGCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAg                                                                                                                                                                <  1:224262/239‑1 (MQ=255)                                                                                                                          ggtgtTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGAttt                                                                                                               >  1:287065/2‑250 (MQ=255)                                                                                                                                           aaaagaacccgttccccAGCACAACCCCCGGCAGGGCGGGGATAAACATGCCCCCCATCCCCAGCGCCGGCCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCAcc                                                                                             <  1:134244/236‑1 (MQ=255)                                                                                                                                           aaaaGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCAcc                                                                                             >  1:289323/1‑251 (MQ=255)                                                                                                                                                   ccGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAgca                                                                                      >  2:183480/1‑248 (MQ=255)                                                                                                                                                      gTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACgg                                                                                  >  2:289322/1‑251 (MQ=255)                                                                                                                                                           cAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGgc                                                                              >  1:139966/1‑250 (MQ=255)                                                                                                                                                            aGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGtagc                                                                              >  2:111021/1‑246 (MQ=255)                                                                                                                                                                                gAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGc                                                         >  1:30012/1‑250 (MQ=255)                                                                                                                                                                                 aGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGcac                                                       >  1:252383/1‑251 (MQ=255)                                                                                                                                                                                     ccacctgccccctcaccccaccccccTGACCCGCAAGCATTTTCTGCCGCGCCCGAGGTTCGCGATTGCTCCATAGCAGCATCATGGTCCGCCCTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCAGCGACGATCACCGCCAGCAACGGTGGCAGCAATAGCAGCAGCGCCAGCACAAtt                                                   <  1:106989/227‑1 (MQ=255)                                                                                                                                                                                                 tCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCg                                       >  1:320276/1‑251 (MQ=255)                                                                                                                                                                                                  cATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGCTCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGc                                      >  2:170393/1‑251 (MQ=255)                                                                                                                                                                                                     ctcCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCCGCGCCAGCACAATTAACACCGTGTCGCaa                                    >  2:242594/1‑250 (MQ=255)                                                                                                                                                                                                     ctcCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGcca                                    >  2:242598/1‑248 (MQ=255)                                                                                                                                                                                                         agcgccTGACCCGCCAGCATTTTCTGCCGCGCCCGACGTTCGCGACTGCTCCATAGCAGCATCCTGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTTGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCAAGCACAATGAACACCGTGTCGCAAATGCg                               >  2:236399/5‑251 (MQ=255)                                                                                                                                                                                                               tGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATg                         >  1:47884/1‑251 (MQ=255)                                                                                                                                                                                                                gACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCCGCACTACGCACAATACACCTGCCGCCAGCGCCATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGCGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCACCAGCAACGGTGGCAGCCACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGAAAATGCGGcgctgc                        >  2:190640/1‑246 (MQ=255)                                                                                                                                                                                                                 ccccGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGTTCAGCCCTACGCACAATACCCCTGCCGCCAGCGCAATACGCAACGAGGCCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTCCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCa                       <  1:186609/250‑1 (MQ=255)                                                                                                                                                                                                                                      cgcCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGACTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGcc  >  1:183253/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     ACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCC  >  NC_000913/73210‑73689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑