Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,121	G→A	A467A (GCC→GCT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,121	0	G	A	100.0%	182.9 / NA	54	A467A (GCC→GCT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

AACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGC  >  NC_000913/72876‑73367                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aaCGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCac                                                                                                                                                                                                                                                   >  2:97801/1‑250 (MQ=255) aaCGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAg                                                                                                                                                                                                                                                   <  2:25141/251‑1 (MQ=255)  aCGCATCGGCAAATGTTGGTAAAGCCAGGTGATAGCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAgc                                                                                                                                                                                                                                                  <  2:59185/251‑1 (MQ=255)                 ttggtAAAGCCAGGTGATATAAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTgg                                                                                                                                                                                                                                    >  1:111770/2‑250 (MQ=255)                    gtAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGt                                                                                                                                                                                                                                <  2:257909/251‑1 (MQ=255)                           cAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAggg                                                                                                                                                                                                                         <  1:194837/251‑1 (MQ=255)                            aGGTGATCTCAGTCAGTTTTAACATCTCGACCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGgc                                                                                                                                                                                                                        >  2:18887/1‑251 (MQ=255)                               ggATATAAGTCAGTTTTAACATCCCGCCCCGGTAGTTTTTCAATCACGGAAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGTAGGGTGCGGAAATCATCGTTACCGAACA‑AAGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCAAGTGGACGTTTCAGGgcgcg                                                                                                                                                                                                                     <  2:273238/250‑1 (MQ=255)                                gATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAACCAGCAGAAAACAGAGTAGCAGCAGACTTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGGGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCACCAGTGGACGTTTCAGGgcgcgc                                                                                                                                                                                                                    >  2:129031/1‑251 (MQ=255)                                   atCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGc                                                                                                                                                                                                                 <  1:156624/251‑1 (MQ=255)                                     cagtcagtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTc                                                                                                                                                                                                               <  1:110873/251‑1 (MQ=255)                                                       cGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCg                                                                                                                                                                                                <  1:37769/248‑1 (MQ=255)                                                          cccGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCa                                                                                                                                                                                          <  2:23178/251‑1 (MQ=255)                                                                        aaTCACGGGAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATgccc                                                                                                                                                                             >  2:120917/1‑250 (MQ=255)                                                                         aTCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACTGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCCGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATgccc                                                                                                                                                                             >  2:224262/1‑249 (MQ=255)                                                                           cACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCg                                                                                                                                                                         >  1:248334/1‑251 (MQ=255)                                                                                 aaaCAGCAGAACATAGAGTAGCAGCAGAATTAAGGCGGTGACCGCGCCGTCCTGGCTGCGAAAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTCCGGACATCATCGTTACCGAACA‑ACTCCACCACGCCCAAATAAACAATCGACAGCACGCAGGCAAAAGCCAGTGCCTGCGCCAGTGGACGTTGCAGAGCGCGCCGCTCGACCACTTTAAAGCGCGCCCAGCCTTAAATGACCAGCGCCTGa                                                                                                                                                                    >  2:218778/1‑250 (MQ=255)                                                                                  aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGAc                                                                                                                                                                   >  1:207881/1‑250 (MQ=255)                                                                                                 agtagcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAgcg                                                                                                                                                   <  2:207881/248‑1 (MQ=255)                                                                                                 agtagcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAg                                                                                                                                                     >  2:233117/4‑249 (MQ=255)                                                                                                                    cgGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGGGATATCGCGCATc                                                                                                                                <  1:321057/251‑1 (MQ=255)                                                                                                                     ggTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCg                                                                                                                               >  1:117139/1‑251 (MQ=255)                                                                                                                                cGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAgt                                                                                                                    <  2:74788/251‑2 (MQ=255)                                                                                                                                   ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGACACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACt                                                                                                                 >  1:185914/1‑251 (MQ=255)                                                                                                                                           cGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCTACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAgcgc                                                                                                         >  1:54515/1‑251 (MQ=255)                                                                                                                                             aTAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCAt                                                                                                       <  1:74502/251‑1 (MQ=255)                                                                                                                                                 gAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGc                                                                                                                                                                          >  2:143156/1‑180 (MQ=255)                                                                                                                                                 gAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGc                                                                                                                                                                          <  1:143156/180‑1 (MQ=255)                                                                                                                                                     cAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTCCCGAACA‑ACGCCCCCACGCCAAAACCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGCGCGCGCATCTCGACCACTTGTAAGCCCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCTCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATc                                                                                               <  1:151928/251‑1 (MQ=255)                                                                                                                                                          ggCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCAt                                                                                          <  2:117139/250‑1 (MQ=255)                                                                                                                                                             gggTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCGGCTCGACCACTTTTAAGCGCGAACAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCACTaa                                                                                       <  2:215543/250‑1 (MQ=255)                                                                                                                                                               gTAGAGAGAAAACGGCAGGGAGCGGAAAGCATCGTTACCGAACACAC‑CCACCTCGCCAAAAGCTCCAATCGACAGCGCGCAGGCTAAAGCCATCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACTCAACATACTGGAGCGGGCGGTGATATCGCGCATCGGGTTTTGCAGTACTCTCAGCGCATAAGGGAGCTCCGTTAACg                                                                                     <  2:44774/251‑1 (MQ=255)                                                                                                                                                                 agagATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAACGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGc                                                                                    >  1:169529/1‑250 (MQ=255)                                                                                                                                                                     aTAAAACGGCAGGGTGAGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCCGCGCCAGTGGACGTTTCTGGGCGCGCAGCTCGATCACTTTTAAGCGCGACCAACCTTCAATGCTCAGCGACTGACACAACATACTGTATCGAGCGGTTATATCGCGCATCGGGTTATCCAGTACTTCCAGCGCATAAGGGATCGCCCTTaaatccttgg                                                                               >  2:71366/1‑243 (MQ=255)                                                                                                                                                                          aCGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCCTTAACGCATTGGTGaa                                                                           >  2:99772/1‑250 (MQ=255)                                                                                                                                                                            ggCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAAt                                                                        >  1:47724/1‑251 (MQ=255)                                                                                                                                                                             gCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATc                                                                       <  1:84819/251‑1 (MQ=255)                                                                                                                                                                             gCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATc                                                                       <  2:135702/251‑1 (MQ=255)                                                                                                                                                                                  gTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACAGACTGTAGCGGGCGGTGATATCGCGCATCGTGTTTTCCAGTACTTTCAGCGCATAGGGGATCGCCATTAACGCATTGGTTAAAATCACAAt                                                                  <  2:197305/251‑1 (MQ=255)                                                                                                                                                                                      ggAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATCAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCg                                                              >  2:77486/1‑251 (MQ=255)                                                                                                                                                                                                   aCCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTGTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg                                                 <  2:272170/251‑3 (MQ=255)                                                                                                                                                                                                     cGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTTGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGTGTTGTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGc                                               <  1:158164/251‑1 (MQ=255)                                                                                                                                                                                                      gAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGc                                               >  1:265064/1‑250 (MQ=255)                                                                                                                                                                                                         ca‑acGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGAGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACCACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGc                                           >  1:319676/1‑251 (MQ=255)                                                                                                                                                                                                          a‑acGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGcc                                          >  2:18791/1‑251 (MQ=255)                                                                                                                                                                                                            acGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTTGTGAAAAGCACAATGCCGTCAGCAGATTGCGGCAGGcca                                         <  1:265411/251‑2 (MQ=255)                                                                                                                                                                                                            acGCCACCACGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGcc                                          >  1:82455/1‑250 (MQ=255)                                                                                                                                                                                                                 accacGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggt                                    >  1:124507/1‑245 (MQ=255)                                                                                                                                                                                                                     cGCCAAAATCACCAATCGACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGtgttg                                <  1:204824/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGcc            >  2:187047/1‑251 (MQ=255)                                                                                                                                                                                                                                               caggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTa        <  1:17064/246‑1 (MQ=255)                                                                                                                                                                                                                                               caggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTa        >  2:17064/4‑249 (MQ=255)                                                                                                                                                                                                                                                aggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGcc     <  1:224638/249‑1 (MQ=255)                                                                                                                                                                                                                                                   cAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGc  >  1:204996/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACA‑ACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGC  >  NC_000913/72876‑73367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑