Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,322	T→C	V202V (GTA→GTG)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,322	0	T	C	100.0%	136.7 / NA	40	V202V (GTA→GTG)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

ATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCAT  >  NC_000913/72087‑72561                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                atatTTTGCATAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTg                                                                                                                                                                                                                                  <  1:258067/251‑1 (MQ=255)             aaGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCgcgcg                                                                                                                                                                                                                      >  1:96958/1‑250 (MQ=255)                             ggggattgTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCCTATCAGGCCTACAAAACGCCCTGTCCGTAGGTCGGATCAGGTGTTCACGCCGCATCTGACAGTGAATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGAATCTTCCACACtgt                                                                                                                                                                                                      >  1:214564/8‑251 (MQ=255)                                gatggTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACtgtgtg                                                                                                                                                                                                   <  1:117235/247‑3 (MQ=255)                                             gggTTGCCTGATGCGACGCTGACCGCGTTTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAACACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATaa                                                                                                                                                                                      <  2:219435/251‑2 (MQ=255)                                                  gCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTc                                                                                                                                                                                  >  1:27910/1‑250 (MQ=255)                                                  gCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCACATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTcc                                                                                                                                                                                 >  2:154279/1‑250 (MQ=255)                                                      gATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCAtt                                                                                                                                                                               <  2:262255/249‑1 (MQ=255)                                                            acgctgaccGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCCGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTtgctg                                                                                                                                                                       >  2:231267/8‑251 (MQ=255)                                                                 gaccGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGc                                                                                                                                                                  <  1:238302/249‑1 (MQ=255)                                                                           tATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCt                                                                                                                                                        >  2:27536/1‑251 (MQ=255)                                                                              cAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCAc                                                                                                                                                     <  1:262937/251‑1 (MQ=255)                                                                                     aCAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTc                                                                                                                                              >  2:260415/1‑251 (MQ=255)                                                                                            gcactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCAtct                                                                                                                                       >  1:311285/8‑251 (MQ=255)                                                                                              actgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTcc                                                                                                                                     >  1:4314/6‑251 (MQ=255)                                                                                                    cGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCAGCTTCCACACTGGGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCGCCTGACGt                                                                                                                               <  1:319281/251‑1 (MQ=255)                                                                                                                aaGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATc                                                                                                                    <  1:244225/250‑1 (MQ=255)                                                                                                                          cgccgcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTGACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAgaga                                                                                                         <  1:137005/251‑1 (MQ=255)                                                                                                                              gcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTACCGCCGGATCGAGCGCAGAGAACgg                                                                                                     >  2:100065/1‑251 (MQ=255)                                                                                                                                    gACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGtt                                                                                                   <  2:75368/247‑1 (MQ=255)                                                                                                                                        gTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGc                                                                                           >  1:97413/1‑251 (MQ=255)                                                                                                                                           cATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAAt                                                                                        <  2:1863/251‑1 (MQ=255)                                                                                                                                                  accagTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGGGACACCACCACTAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTAACCAGCGTCACCATCTCCTGAAACAACGCCGGATCGAGAGCAGAGAACGGTTCACCTCGCAATACAATCg                                                                                 >  2:234572/5‑251 (MQ=255)                                                                                                                                                         aTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTc                                                                          >  2:194837/1‑251 (MQ=255)                                                                                                                                                           ccccAATAGTGCTGAAGCACTCGCCTTAACGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgc                                                                        >  1:118615/1‑251 (MQ=255)                                                                                                                                                               aaTAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcgcac                                                                    >  1:59185/1‑248 (MQ=255)                                                                                                                                                                      ctgAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACa                                                              >  1:16713/3‑250 (MQ=255)                                                                                                                                                                        gAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCTACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTcgctcacctgacaa                                                             <  2:214564/249‑12 (MQ=255)                                                                                                                                                                        gAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAAc                                                            <  2:103738/250‑1 (MQ=255)                                                                                                                                                                         aaGCACTCTCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGGGGCATCGTCCAca                                                                                                                                                                                                          <  2:44555/107‑2 (MQ=255)                                                                                                                                                                         aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAcc                                                                                                                                                                                                          >  1:44555/1‑106 (MQ=255)                                                                                                                                                                         aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTATGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAAcgc                                                          <  2:280017/251‑1 (MQ=255)                                                                                                                                                                              cTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCAAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTa                                                      >  1:286097/1‑250 (MQ=255)                                                                                                                                                                                  ccTTACCGCGCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGcc                                                 <  2:16713/251‑1 (MQ=255)                                                                                                                                                                                           tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACAGAGCGCGAGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACAAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAAAGCGCTAAcacaacgagctgt                                        >  1:44774/1‑239 (MQ=255)                                                                                                                                                                                            caacaaCTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgctgtc                                       <  1:25037/251‑1 (MQ=255)                                                                                                                                                                                               caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGAGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgctgtcgct                                    >  1:215543/1‑251 (MQ=255)                                                                                                                                                                                                    cATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGAc                                 <  1:116403/249‑1 (MQ=255)                                                                                                                                                                                                        ggTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCgg                           >  1:255433/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAt  >  2:121776/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                ATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCAT  >  NC_000913/72087‑72561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑