Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,386	C→T	S12S (TCC→TCT)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,386	0	C	T	100.0%	95.6 / NA	30	S12S (TCC→TCT)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/19);  total (11/19)

CAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACT‑GGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAG  >  NC_000913/71158‑71626                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCg                                                                                                                                                                                                                               <  2:199210/250‑1 (MQ=255)               gcgggTATTTAAAAAATGAACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGcc                                                                                                                                                                                                                >  2:11203/5‑251 (MQ=255)                cgggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCt                                                                                                                                                                                                               >  2:20470/4‑251 (MQ=255)                     aTTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGAGAAGACAAAGTGAATGATGTAGCCGTCAAGTTCTCATAATTGGTAACGAATCAGACACTTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACCCTTTATTACCCAATCTACAGTGTATTCATTGATGGCGGTGGTTTtgttggtttttctt                                                                                                                                                                                                          >  2:145906/1‑243 (MQ=255)                         aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAg                                                                                                                                                                                                       <  1:218335/250‑1 (MQ=255)                              aTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTAACGTGTATTCATTGATGGCGGTGGTGTTTGTGGCCTTTCTGCAGTcgctg                                                                                                                                                                                                 >  2:55944/1‑251 (MQ=255)                                 cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAACGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGGTGGTGGCCTTTCTGGAGTcgctggcg                                                                                                                                                                                              >  2:7216/1‑251 (MQ=255)                                                gagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGAtt                                                                                                                                                                               <  2:303266/251‑1 (MQ=255)                                                             gCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGCAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATCGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACgg                                                                                                                                                                  >  1:201230/1‑251 (MQ=255)                                                                   tgtgAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTGCTGGAGTCGCTGGCGCTGGTCCGTTTGATTCTACCCGGTACGGTGCTg                                                                                                                                                             <  2:197221/250‑1 (MQ=255)                                                                          aaaaaGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACggggaggaggaaggg                                                                                                                                                     <  1:81033/251‑14 (MQ=255)                                                                               gTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTgg                                                                                                                                                >  1:98859/1‑251 (MQ=255)                                                                                          aGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGAGCTGAtt                                                                                                                                     <  2:175008/251‑1 (MQ=255)                                                                                                     tgttaTAATTGGTAACGAATCAGACAATTGATGGCTTGACGGAGTAGCATAGGGTTTGCCGAATCCCTGCTTCGTCCATTTGACAGGCACATGATGCAAGCATTGCTGGAACTCTGTATTTCCCAATCTCCCGTGTAATCATTGATGGCGTTGGTGTTGGTGGCCTTGCTGTAGTCGCTGGCGGGGGTCGGTTTGATTCTACCCGGGATGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAgcggcg                                                                                                                           <  1:127713/246‑1 (MQ=255)                                                                                                          tAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTg                                                                                                                                                                                                                                     <  2:18268/139‑1 (MQ=255)                                                                                                          tAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTg                                                                                                                                                                                                                                     >  1:18268/1‑139 (MQ=255)                                                                                                              tGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGGTTGGCCGCGGCGAGtttaggtt                                                                                                                 >  1:240330/1‑245 (MQ=255)                                                                                                                      aaTCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACg                                                                                                        <  1:216276/251‑1 (MQ=255)                                                                                                                               aTTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCctggctgg                                                                                               <  2:263099/251‑1 (MQ=255)                                                                                                                                             ggAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGGATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGattgtcggt                                                                                 <  2:282919/251‑7 (MQ=255)                                                                                                                                                                aGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGa                                                             <  1:94349/251‑1 (MQ=255)                                                                                                                                                                             cGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATTGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTTGCTGGCAGGGATTGTCGGTTGTCTGCTGGTCGACT‑GGATCTCCTTctggct                                                <  2:263874/251‑1 (MQ=255)                                                                                                                                                                                          ggCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTAATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGtttt                                   <  1:204832/251‑1 (MQ=255)                                                                                                                                                                                                 tATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAg                             <  1:20470/250‑1 (MQ=255)                                                                                                                                                                                                 tATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCGGGGAGCGCTGATTTGCAGCGGGGAGGTAAGGTTTC‑GGCACGCCTGGCTGGCAGGGAGTGTCGGGTGTCTGATGGGCGAAT‑GGAGATCCCTTTGGGTGGGGTGGGGTTTTAAAAAGc                            >  1:234329/1‑251 (MQ=255)                                                                                                                                                                                                       agcgttGCTGGAACACTTTATTACCCAATCTCCCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGTTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGc                      <  2:248672/247‑1 (MQ=255)                                                                                                                                                                                                       aGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGTCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGc                      <  2:248679/251‑1 (MQ=255)                                                                                                                                                                                                            tGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAG‑TTTCTTGCACGCCTGGCTGGGCGGGGTTTTCGGGTGTCTGCTGGGCGACTTGGTCTTCTT‑CTGGCTGGGGTGGGGTTTTTAAAAACCGTTGCAttgc                 >  1:232006/1‑248 (MQ=255)                                                                                                                                                                                                                    cacTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGGCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCAtt         <  2:259914/251‑1 (MQ=255)                                                                                                                                                                                                                            taCCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACT‑GGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTgaag  <  1:259353/250‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAG‑TTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACT‑GGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAG  >  NC_000913/71158‑71626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑