Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,629	G→A	N140N (AAC→AAT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,629	0	G	A	100.0%	123.1 / NA	35	N140N (AAC→AAT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/22);  total (13/22)

CCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAG  >  NC_000913/69406‑69835                                                                                                                                                                                                                                |                                                                                                                                                                                                                cccAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGt                                                                                                                                                                                       >  1:34384/1‑250 (MQ=255)        ttCGTGCCACAGAGATTTAGGCCGGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACAATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAAtctc                                                                                                                                                                                 <  1:118187/249‑1 (MQ=255)                               cGGCGCTACAACGCCCGGGACGAAAACCCGGGGGGGGGGGGGTACCGGAAGGCGGAGCGGGCCCCCAGTCGCACAGCTAAACCCACGGTGCGGCAGATGGCGCCACGGCGCTGTCCTGGAGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCGGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTgg                                                                                                                                                       <  2:195946/251‑1 (MQ=255)                                ggCGCGGCAACGCCCGCGACGAATATCCTGCGGGCGGGTGGTCCCGGAAAGCAGGGCGGGCACCCAGTCGCACAGCTCAATCCACGAGGCGGCAGATGGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTGGCCAAGAACCATTCGCTGGAATAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGt                                                                                                                                                      <  2:156720/251‑1 (MQ=255)                                  cgctacaactgcggggaagaATACCCTGCGGGGGGGTGGTACCGAAAAGCAGGGCTGGCCCCCAGTCGCACAGCGCAATCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCTTTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCt                                                                                                                                                    <  1:284529/233‑1 (MQ=255)                                   gcTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCtt                                                                                                                                                   >  1:71551/1‑251 (MQ=255)                                        aaCGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCcac                                                                                                                                              >  1:237129/1‑251 (MQ=255)                                         ccgcccgggacgaATCTCCTGGGGGGGGGTGGTCCCGGAAAGCAGAGCTGCCACCCAGTCCCACAGCTAAACCCACGAGGCGGCAGATTGCGCCACGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCGGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCcaca                                                                                                                                             <  2:78771/243‑1 (MQ=255)                                                          cctggggggggggtgtACCGGAAGGGAGGGCTGGCACCCATCCCCACCGCTCAACCCGCGATGCGGGAGTGTGCGCCACGGCGCTGCCCTGGCGAGTCACATGAAGGTTTTTTGCCCAAAAACATTCGCTGAAAAAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTc                                                                                                                            <  2:286239/237‑1 (MQ=255)                                                             gcgggcggGTGGTACCGGAAAGCGGGGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCCCGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTGGCCCAGACCCTTTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCgg                                                                                                                          <  1:264497/250‑1 (MQ=255)                                                             gcgggcggGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCggg                                                                                                                         >  2:88510/1‑251 (MQ=255)                                                             gcgggcggGTGGTACCGAAAAGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCgg                                                                                                                          <  1:78032/250‑1 (MQ=255)                                                                    ggtggtACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCgg                                                                                                                  >  2:123779/1‑251 (MQ=255)                                                                     gtggtACCGAAAAGCAGAGCTGGCCCCCAGCCGCACAGCTCAATCCACGAGGCGGCAGATTGCGCCGCGGCGCTGCCCTGGCGAGTCACAGGAAGGATTTTTGCCAAGAACCATTGGCGGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGc                                                                                                                 <  2:26001/251‑1 (MQ=255)                                                                      gggtcccgaaaaccAGAGCTGGCACCCAGTCGCACAGCTCAATCCAGGAGGGGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCa                                                                                                                <  2:203206/238‑1 (MQ=255)                                                                        gtACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCaa                                                                                                               >  1:227474/1‑250 (MQ=255)                                                                         tACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCaaa                                                                                                              >  2:176116/1‑250 (MQ=255)                                                                                aaGCAGAGCGGCCCCCCAGCCGCACAGCCAAATCCACGATGCGGAAGATGGCCCCACGGCGCTGCCCGGGCGAGTCACATGCAGTATTTTTGCCCAAAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGTAAACTCCGGgc                                                                                                      <  2:34384/251‑1 (MQ=255)                                                                                          ggccccctgTCGCACAGCTCAACCCACGATGCGGCAGATTCCGCCACGGCCCTGTCCTGGGGAGTCACATCCAGGATTTTTGCCCAGAACCAATCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAg                                                                                            <  1:117766/243‑1 (MQ=255)                                                                                                      cacacctcaacccacgcgggggCATATTGCGCCACGGCGCTGTCCGGGCGAGTCACATGAAGGTTTTTTGCCCAGAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTc                                                                                <  2:278539/231‑1 (MQ=255)                                                                                                      cacaGCACAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTc                                                                                <  1:68198/251‑1 (MQ=255)                                                                                                       acaGCTCAACCCACGATGCGGCAGATGGCCCCCCGGCGCTGTCCGGGCGAGTCACATGCAGGATTTTTGCCCAGACCCATTCGCTGAAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCg                                                                               <  1:113877/251‑1 (MQ=255)                                                                                                           cacaagccccgaggCGGCAGATGGCGCCGCGGCGCTGTCCGGGGGAGTCAAAGGCAGGTTTTTTGCCAAGAACCATTCGTGGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCAt                                                                           <  2:194320/238‑1 (MQ=255)                                                                                                              aaTCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                      <  1:5498/189‑1 (MQ=255)                                                                                                              aaTCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                      >  2:5498/1‑189 (MQ=255)                                                                                                              aaTCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCa                                                                         >  1:321564/1‑250 (MQ=255)                                                                                                                              gctggcgcCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGAGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCCGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCg                                                         >  2:90245/5‑250 (MQ=255)                                                                                                                                           cgcTTTCCTGGCGAGTCAAAGGCAGGTTTTTTGCCCAGAACCTTTCGCGGAAATAAAGACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCa                                           <  1:261349/251‑1 (MQ=255)                                                                                                                                            ggtgtccgggcgagtaaaaggaagtaTTTTGGCCCAAAACCATCCGCGGAAAAAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCaa                                          <  2:236684/226‑1 (MQ=255)                                                                                                                                                    ggctcttcacttgctgtttttttCCCCAGACCCTTTCGCTGGAATAATTACCGCCAATATAGCGGTAGTAGTCATCATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCATTGTGTTCTTTCCTCAATACGAACATCGCTTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCATTCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATc                                  <  1:220687/233‑1 (MQ=255)                                                                                                                                                                  aGGATTTTTGCCAAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTgc                    >  1:279493/1‑251 (MQ=255)                                                                                                                                                                         ttcccaaGAACCTTGCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCTCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTg              <  1:150910/244‑1 (MQ=255)                                                                                                                                                                             ccAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCCCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCg          >  2:294091/1‑250 (MQ=255)                                                                                                                                                                                    catccGCTGGAATAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAg  <  1:176116/247‑1 (MQ=255)                                                                                                                                                                                    cATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACACACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTTCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTa    >  1:201244/1‑249 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                CCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAG  >  NC_000913/69406‑69835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑