Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG50.0% 3.3 / 10.3 24V288G (GTG→GGG) cusAcopper/silver export system RND permease
Reads supporting (aligned to +/- strand):  ref base T (1/11);  new base G (12/0);  total (13/11)
Fisher's exact test for biased strand distribution p-value = 9.61e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCG‑ACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCT  >  NC_000913/599333‑599783
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                  
aaaGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATc                                                                                                                                                                                                             <  2:247128/251‑1 (MQ=255)
  aGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCt                                                                                                                                                                                                           <  2:61125/251‑1 (MQ=255)
    cgcgcTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCTGGGTGTTGATCCTGc                                                                                                                                                                                                         <  2:182414/251‑1 (MQ=255)
     gcgcgtgaggCTTCAAACCAGGAAGCGGACGGTTGGTCGATCGAACGGGCGGAGGCGGAAGATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGTCGGCTTTAATCACATCGTTTTAAATGCCAGTGAAAATGGCGTGCCCGTGTATCTGCGCGATGGTGCGAGGGTCCGGTTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAGACTGCGAGGGTGAAGTGGCGGTCGGGGTGGTTATCCTGCg                                                                                                                                                                                                        <  2:297482/242‑1 (MQ=255)
             tGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtgggggttcctcgctccggcc                                                                                                                                                                                                >  1:132195/1‑233 (MQ=255)
                                           gatcgaACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGGGAAGGGATTGccgccgggg                                                                                                                                                                  >  1:112720/1‑248 (MQ=255)
                                                                                aGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCTGCGGTCCGGCCAAAAAGCCCGGGAAGGGGTTGCCGCCGGG‑GAGGGCAAACCGGGAACGCGTGAAA‑GTAGTccggccgg                                                                                                                           >  1:43411/1‑244 (MQ=255)
                                                                                           gCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAGACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGga                                                                                                                  <  2:223737/249‑1 (MQ=255)
                                                                                                  cTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGAAGTCTGCCGGGAGGCGGGGAGATAGTTac                                                                                                         >  1:216814/1‑251 (MQ=255)
                                                                                                        gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCTCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGTTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACTC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATa                                                                                                   <  1:297812/251‑1 (MQ=255)
                                                                                                            ttAAGCACATCGTTTTAAAAGCCAGTGAAAATGGCGGGCCCGTTTATCTGCGCGATGTTGCTAAGGGCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAGCGCCGGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGAGGAGATAGGTACAACATAc                                                                                                  <  2:195237/248‑1 (MQ=255)
                                                                                                             tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                                                                               <  1:30968/250‑1 (MQ=255)
                                                                                                                        ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAgg                                                                                                                        <  1:264685/214‑1 (MQ=255)
                                                                                                                        ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGCAAAAACGCCCGAGAAGAGATCGCCGCCGCG‑GAGGGCAAACTGGAAACGG‑CGAAAAAGAGTCTGCCGGGAGGGGGGGAGATAGTTACAACATACGATCCCAGCCAGCTc                                                                                   >  1:280683/1‑251 (MQ=255)
                                                                                                                          ttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCAGGGGCATTGACGAACTAAACGGCGACGGCGAAGTGGCGGACCGGGGGGGGAACCTGCGATCCGGACAAAACGCCCGAGAAGCGGTAGCCGCCGCG‑GAGGACAAAATGGGAACGC‑TGGAAAGTAGTCCGCCGGGAGGCGTGGGGGTAGCTACAACAAACGATCGcagcccactcat                                                                                 >  1:180997/1‑244 (MQ=255)
                                                                                                                                                   ccGTGTATCTGCGCGATGGGGCGAAGGTCTAGAGTGGCCCGGAGATGCGCCGGGGCGTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCAGGGTGGTGATCATGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGGTACAACATACGATCGCAGCCAGCTCATTGCCCGCGCCATCG‑ACAACCTCAGc                                                       <  2:259623/251‑1 (MQ=255)
                                                                                                                                                    cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGAATCTGCGATCCGGCAACAACGCCCGAGAAGTGATCGCCGCCGTG‑AAAGACAAAATGGGAACGC‑TGACAAGAAGTATGCCGGCAGGGGTGGGGCTAGTTACAACATACGCTCGCAGCCACCTCCTTGACCCCGCCATCC‑ACAACCTCaccg                                                      >  1:43031/1‑248 (MQ=255)
                                                                                                                                                           cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCCAAAAAGCCCGAGAAGTGATTGCCGCCCTG‑AAGGACAAACTGGAAAAGC‑TGAAAAGTAGTCTGCCGGAAGGGGGGGGGATAGTTACAACAAACGATCGCAGCCAGCCCCTTGGCCGCCCCCTCGGCCAACC‑CAGCGGCAAGtt                                               >  2:118591/1‑251 (MQ=255)
                                                                                                                                                                    gTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCG‑ACAACCTCAGCGGCAAGTTGCTGGAaga                                      <  2:175230/251‑1 (MQ=255)
                                                                                                                                                                    gTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGGAAAAACGCCCGAGAAGTGATCGCCGCCGGG‑AAGGACAAACTGGAAAAGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGGTAGTTACAACAAACGATCGCCGCCAGCTCATTGACCGCGCCATCG‑GCAACCTCAGTGGCAAGTTGCTggg                                         >  1:169230/1‑247 (MQ=255)
                                                                                                                                                                             gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGGGGAGGGATCGCCGCCGGG‑GAGGACAAACTGGAAAAGC‑TGAAAAGTAGTCTGCCGGAAGGCGGGGAGGTAGTTACAACATACGATCGCCGCCAGCCCATTGAACGCGCCATCG‑ACAACCCCAGCGGCAAGGTGCTGGGAGAGTTTATtgg                             >  1:243119/1‑250 (MQ=255)
                                                                                                                                                                               ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCCGG‑AAGGACAAACTGGAAACGC‑TGAAAAGGAGTCTGCCGGAAGGGGGGGGGATAGTTACAACATACGATCGCAGCCAGCTCATTTACCGCGCCATCG‑CCAACCCCCGCGGCCAGATGCTGGGAGCGTTTATTttgg                           >  2:313014/1‑251 (MQ=255)
                                                                                                                                                                               ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCTCGACCCGGCCAAAAAGCCCCCGAAGCGGGCGCCGCCGTGGAGGGAAAACCGGGAAAC‑C‑TGACAAAAAGTCTGCCGGGAGGCGTGGGGGTAGTTACAAAATACCCTACCAGCCAGCTCATTGACCGCCCCCTCG‑GCCACCTCAGCGGGCAGTTGCTGGGAGAGtttttttggg                           >  2:260070/1‑244 (MQ=255)
                                                                                                                                                                                      ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGTGGTCCTGCGATCCGGGCAAAACGCCCGAGAAGTGATCGCCGCCGTG‑GAGGACAAACTGGGAACGC‑CGAAAAGTAGGCTGCCGGAAGGCGGGGGGATAGGTACAACATACGATCGCAGCCCGCTCATTGACCGCGCCATCG‑ACCACCCCAACGGCAAGTTGCTGGGAGAGTTTATTttggggggggg                    >  2:106233/1‑244 (MQ=255)
                                                                                                                                                                                                        ggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCCCCGGG‑AAGGACAAACTGGGAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCCGCTCATTGACCGCGCCATCG‑ACAACCTCAGCGGCAAGTTGCTGGAAGAGGTTTTTGTGGTGGCGGTAGGCTTTGCGCCGttttt  >  1:64645/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                  
AAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTTCGTCGATCGAACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTG‑AAGGACAAACTGGAAACGC‑TGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCG‑ACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCT  >  NC_000913/599333‑599783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑