Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913523,3350TG58.8% 2.7 / 12.2 17G25G (GGT→GGGrhsDprotein RhsD
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base G (10/0);  total (12/5)
Fisher's exact test for biased strand distribution p-value = 3.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATG  >  NC_000913/523086‑523585
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             
aaaaaTGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGgtg                                                                                                                                                                                                                                                              <  1:60565/251‑1 (MQ=255)
              aataaAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATAAGCGGACAACCAGAGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTTCCATTATCCTGGGGTCGGCAGGGGTAAGAATTggtggg                                                                                                                                                                                                                                                >  1:270185/1‑247 (MQ=255)
                                                         tgaGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGGAAGAATTGGCGCGCCCACCCGCGGGGAGTTGTCGGTGTGTCcgggccggggggctt                                                                                                                                                                                                     >  1:105500/1‑241 (MQ=255)
                                                                    aaGTTGATGTGAAAAATATATAAATACATTAGCTGGTCGTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACGTTATATGAGCGGAAAGCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACGTCGGGCATCCCg                                                                                                                                                                                         <  2:109071/251‑1 (MQ=255)
                                                                                                    cTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCAGGGGTAAGAATTTGCGCGCCCACCGGCGGGGCGGGCTCCGGGTGTTCGGGGGGGGTGACCTCGGGGAACCCCGGAAATCCCCTGCCggggggggagggggtgcc                                                                                                                                                         >  1:25555/1‑238 (MQ=255)
                                                                                                                   attttattttTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCCGGGTTCGGCCGGGGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCGCGGGGTGTCCGGGGGGGGTGACTTCGGGGAACCCCGTAAATCCCCCTCTGGGGGCGGAGGTGGTGCCCGGCggggagggccc                                                                                                                                          >  1:206529/1‑241 (MQ=255)
                                                                                                                                ttgttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTTTCCAGGGTTCGGCAGGGGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGGTGACCTCGGGCAACCCGGTAAATCCGCTGCCGGGGGCGAAGGGGCCGCCCGGCGAGGCGGACCTTTCGCTgcccggg                                                                                                                             >  1:79824/1‑250 (MQ=255)
                                                                                                                                       tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGACCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGGGCTCGGTGTGTCCGGGAGGGGTGACCTCGGGCAACCCCGGAAATACGCTGCTGGGGGCGAAGGGGCTGCCCGGCGAGAAGGGACTTGCGCTGACCGGCCCGccgc                                                                                                                      >  1:34517/1‑248 (MQ=255)
                                                                                                                                          cacaGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCAGGGGTAAGAAATGGCGCGCCCACCGGCGTGGCGTGCTCCGGGTGTCCGGGCGGGGTGACCTCGGGCCACCCCGGAAATCCCCTGCTGGGGGCGAAGGGGCGGCCCGGCGGGGCGGGCCCTGGGCCGCCCCGCccgccgccgg                                                                                                                  >  1:218921/1‑250 (MQ=255)
                                                                                                                                                         aaTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCCGGGGTCGGCAGGGGTGAGAATTGGCGCGCCCCCCCGCGTGGCGTGCTCGGGGGGTCCGGGGGGGGTGACTTTGGGCCACCCCGGAAAAACCCCGCCGGGGGGGGAGGGGCTGCCCCGCGAGACGGGCCTTGCGCTGCCCCGCCCCCTGCCgctcctttcctcccccc                                                                                                   >  2:168110/1‑235 (MQ=255)
                                                                                                                                                                    gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGGGTGTCCGGGCGGGGTGACTTCGGGCAACCCCGTAAATCCGCTGCTGGGGGCGGAGGTGCTGCCCGGCGGGACGGACCCTGCGCTGCCCGGCCCGCCGCCGGTCCTTTTCTCCCGCACCTAcagccgc                                                                                       >  1:228883/1‑248 (MQ=255)
                                                                                                                                                                                  aGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCcgggc                                                                                                                                                                                                               <  1:160563/120‑1 (MQ=255)
                                                                                                                                                                                               cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAGAATTGGCGCGCCCACCGGCGTGGCGGGCTCGGTGTGGCCGGGCGGGGTGACTTCGGGCCACCCGGTAAAACCGCTGCTGGGGGGGGAGGTGCTGCCCGGCGCGACGGACCCTGCGCCGCCCGGCCCCCCGCCCGTTAATTTCTCCCCCCACTACATCAGCCACCGGACGAAGACGCCCGCAcccggg                                                            >  1:163861/1‑247 (MQ=255)
                                                                                                                                                                                                                      cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTgg                                       <  2:251799/249‑1 (MQ=255)
                                                                                                                                                                                                                          aTGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGGGTGTCCCGGCGGGGTGACTTTGGGCAACCCGGGAAATCCGCTGCTGGGGGGGGAGGTGCTGCCCGGCGAGACGGACCCTTCGCTGCCCCGCCCGCTGCCGGTTCTTCTCTCCCGCCCCCACCGCAGCTACCCGACGAAGACCCCCGCCCCGGGGGGCGGTTTTGGCCCCCGCTGGGAAgcg                                 >  1:279356/1‑251 (MQ=255)
                                                                                                                                                                                                                                  cccATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGa                         <  1:158942/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggcgggTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTTCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTATCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTAc        <  2:151663/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTAc        >  1:7101/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         tgtAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGAGTGCCCGGGCGGGATGACTTAGGGCAACACGGTAAATCCGCTGCTGGGGGCGAAGGAGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCAGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAAGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGCAAGCGCCTTCTGATATCCGCTTACCGCTACGtgatg  >  1:294398/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             
AAAAATGTTAGTTTAATAAAATATTGAAAGTGACCTGTAATAACAGTTGTTGTTGATTGAGAACAAATAAGTTTATGTGAAAAATATATAAATACATTAGCTGGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGACGAAGACGCCTGCACCGGTGGGCGTTTTCGGCCCCGGCTGGAAAGCGCCTTCTGATATCCGCTTACAGCTACGTGATG  >  NC_000913/523086‑523585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: