Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (+) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	15387 =	NA (NA)	21 (0.670)	19/484	0.7	100%	noncoding (1/1345 nt)	IS186	repeat region
	?	NC_000913	= 458795	0 (0.000)					intergenic (+95/‑93)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
*	?	NC_000913	458790 =	1 (0.030)	17 (0.540)	16/486	0.9	94.3%	intergenic (+90/‑98)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	= 609350	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

GTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTG  >  NC_000913/15140‑15628                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTccc                                                                                                                                                                                                                                                  <  1:8269/251‑1 (MQ=255)         cgAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgcg                                                                                                                                                                                                                                           <  1:129171/250‑1 (MQ=255)                        ccTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTGACTcccccacacgcctcccttaagggtgg                                                                                                                                                                                                                           >  1:249055/1‑228 (MQ=255)                                   ggCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGcc                                                                                                                                                                                                              <  2:249055/251‑1 (MQ=255)                                      aGAAACAGGTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGa                                                                                                                                                                                                           <  1:291900/251‑1 (MQ=255)                                                gctgcaagagctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTa                                                                                                                                                                                                  <  1:302671/250‑1 (MQ=255)                                                          ctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCAATCCCACAAAAGCTAAC‑TTTAAGGGT‑GTGGGATTTCGCCCGATATGtttttaagtggcgagg                                                                                                                                                                                       >  1:317301/1‑239 (MQ=255)                                                                  aaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACCCCCCTAAACGCTAAC‑TT‑AATGGGTGTGGGATTACGCCTGATATGATTTTAAGTGCCGATGAATTaatt                                                                                                                                                                               >  1:270445/1‑248 (MQ=255)                                                                        cGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg                                                                                                                                                                          <  2:247066/250‑1 (MQ=255)                                                                        cGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg                                                                                                                                                                          <  1:187503/250‑1 (MQ=255)                                                                        cGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACg                                                                                                                                                                          <  1:197150/250‑1 (MQ=255)                                                                            ggCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAc                                                                                                                                                                     <  1:144474/251‑1 (MQ=255)                                                                               ccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTgg                                                                                                                                                                  <  2:51897/251‑1 (MQ=255)                                                                                  aCCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCa                                                                                                                                                               <  2:235733/251‑1 (MQ=255)                                                                                      gCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCaa                                                                                                                                                           <  1:262700/251‑1 (MQ=255)                                                                                                   gacgcgCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCatat                                                                                                                                              <  1:15687/249‑1 (MQ=255)                                                                                                                                 gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGc                                                                                                                <  2:317301/251‑1 (MQ=255)                                                                                                                                                                                  cGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCCAAAACGCCAACCTT‑AAGGGG‑GTGGGGATAAGCCCGGTATTGTTTAAACTGGCGAGGAAATACTCCCCAGATAAATGGGCAACAATTCTTGCCCCTATTTGTAAGCCCCCAGAAATGGGTAATTCGGCCAGTAAAGCCGGGGCTCTAACCCCCCGCCGCGAAAATCGTGGtgctgcc                                                               >  1:64181/1‑250 (MQ=255)                                                                                                                                                                                     gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATCTGCGAGCACCTGTAAAGTGGCGGGCAACACCCCCCAAAGAACCAAC‑CC‑AACGGTTGTGGGATTAAGCCTCATATGATTTTACATGCCGCTGGATTACTCCCTCGATAACTGGTCAAAAATTCTCGCCCACATTGGTAAACCCCAAAAACTGGATACTTCCGCACGCAATGCCCGGGGTCTAAACCGCCCCCCCGAAAATTGTCAtgctgccacc                                                            >  1:186468/1‑247 (MQ=255)                                                                                                                                                                                               gggTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACg                                                    <  1:211873/249‑1 (MQ=255)                                                                                                                                                                                                               cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACgg                                  <  1:147760/251‑1 (MQ=255)                                                                                                                                                                                                                    aaGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCg                             <  2:87065/251‑1 (MQ=255)                                                                                                                                                                                                                           gCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGAt                      <  2:73047/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggggATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTg  <  2:281779/251‑1 (MQ=33)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAC‑TT‑AAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTG  >  NC_000913/15140‑15628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.