Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,204	Δ1 bp	coding (1023/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,204	0	T	.	100.0%	145.1 / NA	31	coding (1023/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/23);  total (8/23)

CAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTA  >  NC_000913/1301958‑1302439                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            aacccGCTACCGTAGTGGTGAAATCGACATGACTGATAACAGCATGCCGATCGAATTGTTCCAAAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTa                                                                                                                                                                                                                                        <  2:230327/248‑1 (MQ=255)  aaCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGGACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTac                                                                                                                                                                                                                                       <  2:157808/251‑1 (MQ=255)        tACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGAAGGGCAACATGCCCGCCTA‑GGTTACACTcc                                                                                                                                                                                                                                  >  2:250411/1‑250 (MQ=255)                 ggtgAAATCGACATGACGTATAACAGCAGGCCGATCGAATTTTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACt                                                                                                                                                                                                                        <  2:94599/251‑1 (MQ=255)                  gtgAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTg                                                                                                                                                                                                                       <  1:65509/251‑1 (MQ=255)                    gAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCCCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGAt                                                                                                                                                                                                                     <  1:303327/251‑1 (MQ=255)                        tcgacaggccgtaTAACAGCATGCCGCCCGAATTGATCGAGAAGCTGAAATAAGACATCCAGGACGAAGTTGACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCCTTGTTAATAAAGTGAACGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGgcg                                                                                                                                                                                                                 >  1:238921/13‑251 (MQ=255)                               ggcttaTAACACCATGCCGATCGAATTGTTCCAGAAGTTGAAAAAAGAGACCCCGGACGAAGTTCCCGTTGATCCAGACCTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAAtt                                                                                                                                                                                                          <  2:177918/246‑1 (MQ=255)                                     aaacggCATGCCGATCGAATTGTCCCAAAAGCTGAAAAAAGAGAGCCCGGACGAATTCCACGTTGATCCATCCCTTTGCACTTACTATTACAAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCa                                                                                                                                                                                                    <  2:62455/246‑1 (MQ=255)                                       aCAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGc                                                                                                                                                                                                  >  1:219370/1‑251 (MQ=255)                                                 gATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGtt                                                                                                                                                                                        >  1:247783/1‑251 (MQ=255)                                                                          aaaGAGATCCCGGACGAATTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAAc                                                                                                                                                               <  1:244956/251‑1 (MQ=255)                                                                            agagACCCGGGACGAAGTTCACGTTGGTCCAGCCCGGTTCATTTCCTATTAGAAAATTAACAACAAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACga                                                                                                                                                             <  2:165390/251‑1 (MQ=255)                                                                                 ccccGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAGGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCTCCGTATACTGATGGCGCAAAATTGACGCAGCCATAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGaagaag                                                                                                                                                        <  1:128946/250‑1 (MQ=255)                                                                                  cccGGAAGAAGTTCCCGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATAGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGc                                                                                                                                                       <  2:222321/251‑1 (MQ=255)                                                                                      gACGAATTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGaaa                                                                                                                                                   <  1:180990/251‑1 (MQ=255)                                                                                                 cGTTGATCCATACCTGGGCACTTACTATTACGAAATTAACAACCAGAAACCTCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTAGACTCCACCGTATACTGATGGCGCAAAATTGGCTCAGCCAGAATGGTTGGGCTGGAGCCAGGAAAAGCGTAGCGAAGAAGCGAAAAAACTtctggc                                                                                                                                        <  2:268765/251‑1 (MQ=255)                                                                                                     gATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGa                                                                                                                                     <  1:257792/250‑1 (MQ=255)                                                                                                                       tACTAATACAAATTTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACGAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGAGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGa                                                                                                                   <  2:200252/250‑1 (MQ=255)                                                                                                                                      aacaacCAGAAACCGACATTCAAAGATGTGCGCGTGCGTACCGCACTGAAACTAGGCATGGCCCGCGATATCATTGTTAATAAAGTGACAGAGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACAGCAGACAAACCGTAGACCAtt                                                                                                   >  2:108955/1‑250 (MQ=255)                                                                                                                                               aaaCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCtgttg                                                                                          >  1:213977/1‑251 (MQ=255)                                                                                                                                                  ccGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGtat                                                                                       <  1:1956/251‑1 (MQ=255)                                                                                                                                                     ccATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTTTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAac                                                                                    <  2:9555/251‑1 (MQ=255)                                                                                                                                                       aTTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAacac                                                                                  <  2:98284/251‑1 (MQ=255)                                                                                                                                                           aaCGATGTGCGGGTGCGTACCGCACTGAAACTAGGTAGGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAAAGGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTcc                                                                              <  2:257648/251‑1 (MQ=255)                                                                                                                                                                                        aCTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATtgctgc                                                 <  2:298468/251‑1 (MQ=255)                                                                                                                                                                                               aTGGACCGCGATATCATTGTTAATAAAGGGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCa                                          <  2:92692/251‑1 (MQ=255)                                                                                                                                                                                                       cgATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGga                                   >  1:61770/1‑250 (MQ=255)                                                                                                                                                                                                         atatCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGgaaga                                <  1:49644/251‑1 (MQ=255)                                                                                                                                                                                                                                     gggCAACATGCCCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCCGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGt    >  1:154011/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCAACATGACCGCCTA‑GGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAAGGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAATCTGGTTa  <  1:166612/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            CAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTA  >  NC_000913/1301958‑1302439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑