Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913385,7970TG35.3% 66.1 / 12.5 52V189G (GTG→GGG) tauAtaurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (9/24);  new base G (16/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 2.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGGCTGGTGAAGGGGAATAC  >  NC_000913/385554‑386043
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          
tggtggTAAAGAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTTGGAAAAGACGGC‑AAGGGGTTGAcc                                                                                                                                                                                                                                                     >  1:321370/1‑251 (MQ=255)
          gAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGGTAACGCCCTGGGAAAAGCCGGC‑AAGGGGTTggcccgttcttaac                                                                                                                                                                                                                                           >  1:21682/1‑238 (MQ=255)
                   cAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCggg                                                                                                                                                                                                                                  <  1:267906/250‑1 (MQ=255)
                      cAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAAACGGC‑CAGGGGTTGGCCG‑GTTCTGAACAGGGCGGGCAgg                                                                                                                                                                                                                              >  1:48476/1‑250 (MQ=255)
                        aaCCGGAAGATCTGATTGGCAAACGCATCGCCGGACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACGGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTgg                                                                                                                                                                                                                            <  2:248892/251‑1 (MQ=255)
                              aaGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCTCCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACTGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGgcgcg                                                                                                                                                                                                                      <  1:283590/251‑1 (MQ=255)
                                    tGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCAGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCCGGAAAAAGGCGGG‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGGGGGGCGCGCCAAc                                                                                                                                                                                                                 >  1:262500/1‑250 (MQ=255)
                                         ggCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCAGTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTgg                                                                                                                                                                                                            <  2:421788/250‑1 (MQ=255)
                                           cAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACTG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACg                                                                                                                                                                                                         <  1:289741/251‑1 (MQ=255)
                                              actcctctccGTACCGTTTAGCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCGGCCTGGCAGCGGGGAGATATTGATGGTGCTTAGGTCTGGGCACCGGCTGTTAACGCCCGGGAAAAAGACGGC‑AAGGGGTTGACCG‑AGTCTGTTCAGGTCGGGCAATGGGGCGCGCCAACGCTTCACGTCt                                                                                                                                                                                                      <  2:301413/243‑1 (MQ=255)
                                                gCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTTACGCCCCGGGAAAAGAAGGC‑AAGGGGTTGACCG‑AGTTTGAACAGGGCGGGCGGGGGGGGGCGCCCACCCTGGACGTCTgg                                                                                                                                                                                                    >  1:197322/1‑251 (MQ=255)
                                                 cATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGGTAAACCCCTTGGAAAAGAAGGC‑AAAGGGTTGGCCG‑ATTCTGAACAGGGCGGGGCGTGGGGCGCGccccagctgggcgtccggg                                                                                                                                                                                                   >  1:412256/1‑234 (MQ=255)
                                                         tACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTGTCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCTGGGAAAAAACGGC‑CAGGGGTTGGGCG‑ATTGTGAACAAGTCGGGCCGAGGGGCGCGCCCACGCTGGACGTCCggggggggcgg                                                                                                                                                                                           >  2:44351/1‑243 (MQ=255)
                                                                      caccacCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCCGGGAAAAGACGGC‑AAGGGGGTGACCG‑GTTCTGTACAGGTCGGGCAGGGGGGCGCGCCAACGCTGGACGGCTGGGGGGGGGGCAAAAAATTTGCCg                                                                                                                                                                              >  1:174086/1‑251 (MQ=255)
                                                                                        gctgGCGGCACTGAAACACTGGGGCAGTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCg                                                                                                                                                            <  1:164661/251‑1 (MQ=255)
                                                                                          gggcggcACTGAAACACTTGGGCATTAAACCCGGGCAGGTGGAGATTGTGAACATGCAGCCGCCCGCGATTATCGCTGCCTGGCAGTGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGTC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGAGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTg                                                                                                                                                          <  2:256347/250‑1 (MQ=255)
                                                                                            gcgtcACTGAAACACTGGGTCATTAAACCCGGGCAAGTGGAGATTGTGATCAGGCAGCCGCCCGCGAGTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCTGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGaa                                                                                                                                                        <  2:138880/247‑1 (MQ=255)
                                                                                                    gAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGtt                                                                                                                                                  <  1:293866/249‑1 (MQ=255)
                                                                                                               gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTCAAGCCCCGGAAAAAAACGGG‑AAGGGGTTGACCG‑ATTTTGAACAGGGCGGGGCGGGGGGGGCGCCAACCCTGGACGGCTGGGGGGGGGGCAAAAATTTTGCCGGGGAACATCATGAGGTCGGGaaaaagtttgcttaaaacgcc                                                                                                                                     >  1:233545/1‑233 (MQ=255)
                                                                                                                       cccGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGGCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGc                                                                                                                             <  2:386365/251‑1 (MQ=255)
                                                                                                                             cAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAATGCCCCGGTAAAAGACGGC‑AAGGTGTTGACCG‑AGTCTGGACAAGGCGGGCAGCGGGGCGCGGCAAAGCCGGATGCCTGGGGGGGGCGGAAAGATGTTGGCGAGAAAAATCCAGAGGGGGGGAAAACGGTAGCTAAAAGAGCCATCGagggaccgc                                                                                                                        >  1:259103/1‑242 (MQ=255)
                                                                                                                                  ggAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAAACGGCCAAGAGGTT‑ACCG‑AGTCTGAACAAGGCGGGGAGGGGGGCGCGCCAACGCTGGGCGTCTAGGGGGGGCGCAAATAATTTGCCGGGAAAAAACCTGAGGGTGTGGAAGAGTTCGCTAAAAAAGCCCTCGAAGCCCTGCAA‑CCGt                                                                                                                 >  1:611/1‑251 (MQ=255)
                                                                                                                                   gagaTTGTGAACCTGTAGCCGCCCGCGATTATCGCTTCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCTCCGGCGGTGAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTTAGCAA‑CCGTa                                                                                                                <  2:295265/251‑1 (MQ=255)
                                                                                                                                   gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTg                                                                                                                                                                                 <  2:251715/187‑1 (MQ=255)
                                                                                                                                      aTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCTGGAAAAAGACGGG‑AAGGTGTTGACCG‑ATTCTGAACAGGGCGGGCAGGGGGGGGGGCCAACGCTGGGCGGCTGGGGGGGGGGCCAAGAATTTGCCGGGAAACATCCTGTGGGCGGGGAAGCGTTCGCTAAAAACGCCCTCGATGATCAGCCA‑CCGAAC‑At                                                                                                            >  1:123544/1‑251 (MQ=255)
                                                                                                                                           gAACCTGCAGCCGCCCGCGAGTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCt                                                                                                        <  1:480116/250‑1 (MQ=255)
                                                                                                                                             aCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGGAAAAAAAGGC‑AAAGGGGTGGCCG‑ATTTCGAACAGGTCGGGCAGAGGGGGGCGCCCACGCTGGACGCCTGGGGGGGGGGCAAAAGTTTTGCCGGGGAAAAACCTGAGGGCGGGAAAGGGGTCGCTAAAAAAGCCATCCATGCTCCGCAAACCG‑AC‑AATGCTAAc                                                                                                     >  1:486564/1‑251 (MQ=255)
                                                                                                                                                   aGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGAAAAAGACGGC‑CGAGTGGTGCCCG‑ATTCTGGACAGGTGGGGGAGTCGGGGGCGCCCACCCTGGACGACTGGGGGGGGGGCCACGGTTTTTCCGGGGAACCTCCTGTGGTCGTGGAAACGGTCGCTAAAAACGCCCTCGGTACTCAGCCA‑CCGGGC‑ATTGCTAACCCCGAc                                                                                               >  1:300193/1‑251 (MQ=255)
                                                                                                                                                       gcccgcGATTATCGCTGCCTGGCAGCGGGGCGATATTGATGGTGCTTATGTCTGGGCACCGGCGGGTAACGCCCTGGGAAAAAAAGGC‑AAGGGGGTGACCGGATT‑TGTACCAGTCGGGGAGGGGGGGGGGCCCACGCTGGGCGTCTGGGGGGGGGGCAAAAAGTTTGGCGAGAAACATCCTGAGGGCGTGAAAGCGGTCGCTAAAAGAGCCATCGATGCTCAGAAA‑ACGGAC‑ATTGCTAACCCCGACgtgt                                                                                           >  1:289841/1‑251 (MQ=255)
                                                                                                                                                       gcccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑CAGGGGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCCA‑CCGTAC‑ATTGCTAAACCAGACgtgg                                                                                           >  1:184193/1‑250 (MQ=255)
                                                                                                                                                       gcccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACgtgt                                                                                           <  1:481073/251‑1 (MQ=255)
                                                                                                                                                         ccgcGTTTATCGCTGCCGGGCATCGGGTAGATATTGATGGTGCGTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGGGTTGACCG‑AGTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTGGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCAGCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTgg                                                                                         <  2:366733/251‑1 (MQ=255)
                                                                                                                                                         ccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGGGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGGCGTCTGGGTGGGGCGCAAAGATTTTGCCGAGAAACATCCTGGGGTCGTGAAAGCGTTCGCTAAAAGCGCCCTCGATGCTCAGCCA‑CCGGAC‑CTTGCTAACCCAGACGGGTgg                                                                                         >  1:6260/1‑251 (MQ=255)
                                                                                                                                                                 aTCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACa                                                                                <  1:445794/251‑1 (MQ=255)
                                                                                                                                                                    gCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGcc                                                                                                                                                                               <  1:28580/156‑1 (MQ=255)
                                                                                                                                                                            gCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTGCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAGCCCAGACGTGTGGCT‑GAAACAGCCGGAAAACa                                                                     <  2:907/251‑1 (MQ=255)
                                                                                                                                                                             cAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCCGGGAAAAGACGGC‑AAGGGGGTGGCCG‑ATTTTGAACAGGGCGGGCCGTGGGGGGCGCCAACGCGGGAAGTCTGGGGGGGGGGCAAAGATTTTGCCGAGAAACATCCTGAGGGCGGGGAAGAGGTCGCTAAAAAAGCCCTCGATGCTCAGCCA‑ACGCAC‑ATTGTTCACCCCAACGTGTGGGT‑GAAACAACCGGGAAACAt                                                                    >  2:182635/1‑251 (MQ=255)
                                                                                                                                                                                 ggggAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACACCCTGGAAAAAGACGGC‑AAGGGGGTGACCG‑ATTCTGAACAGGGCGGGCAGGGGGGGGCGCCCACGCTGGACGGCTGGGGGGGGCGCAAAGATTTTGTCGAGAAACATCCTGAGGGCGGGGAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGGAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAACCGGAAAACATCAGc                                                                >  1:47256/1‑251 (MQ=255)
                                                                                                                                                                                  gggAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGAAAAAGAAGGG‑AAGGTGTTGACCG‑CTTCCTAACAGGGCGGGGCGGGGGGGGCGCCCACGCTGGACGGCTGGGGGGGGCGCCAAAATTTTTCCGAGAAACCTCCTGAGGTCGGGAAAGGGTTTGCTAAAAACGCCCACGATGCTCCGAAA‑CCGGACCATT‑CTAAACCACACGTGGGGCTTGAAA‑AGCCGGaaaaaaacaacc                                                               >  2:388640/1‑243 (MQ=255)
                                                                                                                                                                                          ttGATGGAGCTTATGTCTGGGCACCGGCGGCTAAAGACCTTGGAAAACACGGC‑CAGGGGTTTGCCC‑ACTCTGAACAGGGCGGGCCGTGGGGCGCGCCCACGCTGGACGTCCGGGTGGTGCGCAAAGATTTTTGCGAGAAACAACCTGAGGGCGGGGAAGCGTTTGCTAAAAACGACATACATGCTCAGCCA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GCACCAGCCGGAAAACAATAGCAAACTGgcg                                                       >  1:426150/1‑251 (MQ=255)
                                                                                                                                                                                            gATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGgcgc                                                      <  2:452774/250‑1 (MQ=255)
                                                                                                                                                                                                gTGCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGGAACAGACGGC‑CAGGCGATGCCCG‑ATTCTAAAAAGGTCGGGCAGTGGGGAGCCCCCACGCTGGAAGTCCGGGGGGGGGGCAAAGATTTTTCCGAGAAACATCCTGAGGGCGGGGAAGCGTTCGCTAAAAGCGCCCTCCATGCTCCACAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAACCGGAAAACAACAGCAAACTGGCGCGttt                                                  >  1:232239/1‑250 (MQ=255)
                                                                                                                                                                                                            gggCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCt                                     <  1:182635/251‑1 (MQ=255)
                                                                                                                                                                                                                     cggTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGAc                            <  2:123544/251‑1 (MQ=255)
                                                                                                                                                                                                                         tAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACTCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACTTCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACTTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGtt                         <  2:289841/250‑1 (MQ=255)
                                                                                                                                                                                                                          aaCGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTcc                       >  2:267197/1‑251 (MQ=255)
                                                                                                                                                                                                                          aaCGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTcc                       <  2:47256/251‑1 (MQ=255)
                                                                                                                                                                                                                           aCGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGGCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTGTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCg                      <  2:174086/251‑1 (MQ=255)
                                                                                                                                                                                                                             gCCCTGGAAAACGTCGGC‑AAGGTGTTGACAG‑ATTCTGAACAGGCCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGCTTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGAGTGCCTGAAGGTGACGTTCCggg                    >  2:68096/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aaaaaGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCACAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGGCTGGTg             >  1:407557/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ggC‑AAGGTGTTGTCCG‑ATTCGGAACAGGTCGGGCAGGGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTCGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGAGTGCCTGAAGGTGACGTTCCGGGGCTGGTGAAGGGGaa     <  1:68096/251‑1 (MQ=255)
                                                                                                                                                                                                                                                aaGGTGTTGACCG‑ATTCTGAACAGGACGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGGCTGGTGAAGGGGAATAc  >  1:93513/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          
TGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGC‑AAGGTGTTGACCG‑ATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAA‑CCGTAC‑ATTGCTAACCCAGACGTGTGGCT‑GAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGGCTGGTGAAGGGGAATAC  >  NC_000913/385554‑386043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑