Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,901	G→A	D479D (GAC→GAT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,901	0	G	A	100.0%	151.7 / NA	54	D479D (GAC→GAT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/28);  total (26/28)

CTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCC  >  NC_000913/66665‑67140                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                cTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCAc                                                                                                                                                                                                                                   >  1:197409/1‑251 (MQ=21)   tATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCAc                                                                                                                                                                                                                                   <  2:193167/249‑1 (MQ=21)               aaaaTCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGt                                                                                                                                                                                                                     <  2:371042/251‑1 (MQ=25)                           cgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATGCACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCgg                                                                                                                                                                                                         <  2:161705/251‑1 (MQ=25)                                 ccTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaact                                                                                                                                                                                                   <  1:25530/251‑3 (MQ=25)                                  cTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCCTCTCGGCGaactg                                                                                                                                                                                                  >  1:178710/1‑248 (MQ=25)                                    gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCCTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaactg                                                                                                                                                                                                  <  1:36315/249‑4 (MQ=25)                                           cgcgCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCTCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATTGCGGGCAGCTCGGCGAACTGGCGCATATc                                                                                                                                                                                         <  2:162281/251‑1 (MQ=21)                                              gcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCCCGGCGAACTGGCGCATATCGtt                                                                                                                                                                                      >  2:68716/1‑251 (MQ=25)                                                cAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATTACCGTGAGTTCCATGTCGTGCATCTCGGCGAACTGGCGCCTATCGTTgg                                                                                                                                                                                    >  2:62768/1‑250 (MQ=25)                                                    gTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGCATCGTTAACAATCACCGTGATTTCAAAGGCGTGCATCCCGGCGAAATGGCGCATATCGTTGAGGtt                                                                                                                                                                                >  1:355072/1‑251 (MQ=25)                                                              gCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATgg                                                                                                                                                                      <  2:70166/251‑1 (MQ=37)                                                                gTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCCTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCt                                                                                                                                                                    >  1:269995/1‑251 (MQ=37)                                                                         cGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACggg                                                                                                                                                           >  1:204129/1‑250 (MQ=37)                                                                            aTGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGTGATTTCAATGTCGTGCATCTCGGCCAACTGGCGCATATCGTTTAGGTTCAGCGCATGGCTGGAGACGGTAtt                                                                                                                                                        >  2:80104/1‑250 (MQ=37)                                                                                         aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGccacca                                                                                                                                           <  2:141922/251‑1 (MQ=38)                                                                                         aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGCTATCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCCGCGCATGGCTTAAGACGGTATGGTGCGCGccaccc                                                                                                                                           >  2:286133/1‑250 (MQ=38)                                                                                            cGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCg                                                                                                                                        >  1:275643/1‑251 (MQ=38)                                                                                               ccTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATTTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGTGCCACCAGCGAgg                                                                                                                                     <  1:297066/251‑1 (MQ=255)                                                                                                  aCAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATc                                                                                                                                  >  1:81785/1‑251 (MQ=255)                                                                                                          gCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTc                                                                                                                          <  1:222615/251‑1 (MQ=255)                                                                                                           cAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCg                                                                                                                         >  2:242697/1‑251 (MQ=255)                                                                                                               gtgtAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCCCCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATAGTGCGCGCCACCCGCCGGGATCCACGCCTCGGacg                                                                                                                     >  2:85525/1‑249 (MQ=255)                                                                                                                   aGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGt                                                                                                                 <  2:21175/251‑1 (MQ=255)                                                                                                                    ggCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGtt                                                                                                                >  1:1843/1‑251 (MQ=255)                                                                                                                             aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc                                                                                                       <  2:4922/251‑1 (MQ=255)                                                                                                                                 gcgTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCTGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCTTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGtt                                                                                                   <  1:240492/251‑1 (MQ=255)                                                                                                                                         gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTc                                                                                           >  1:321333/1‑251 (MQ=255)                                                                                                                                         gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTc                                                                                           <  1:291915/251‑1 (MQ=255)                                                                                                                                               tCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATTGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGATGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCCCCAGCGAGGATCCACGCCTCGGAAGCAGTTGGCCGATCCGGTTGCGCTTTCCACAgc                                                                                     >  1:249174/1‑251 (MQ=255)                                                                                                                                                 aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGCTGCGCTTTCCACAgcgc                                                                                   >  1:154949/1‑251 (MQ=255)                                                                                                                                                 aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCTCTTTCCACAgcgc                                                                                   <  1:253757/251‑1 (MQ=255)                                                                                                                                                 aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACAgcgc                                                                                   >  2:61976/1‑251 (MQ=255)                                                                                                                                                   gctttACACACCTGATGCGACTACTGAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                                                                 <  2:222758/248‑1 (MQ=255)                                                                                                                                                          acacctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACCGCGCATTCGCCAc                                                                          >  1:102596/7‑251 (MQ=255)                                                                                                                                                            ccctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCg                                                                        <  2:215944/247‑1 (MQ=255)                                                                                                                                                                   gcgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGttt                                                                 <  1:10787/247‑1 (MQ=255)                                                                                                                                                                    cgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCTCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCGTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                                <  2:233259/248‑1 (MQ=255)                                                                                                                                                                              gCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGt                                                      <  2:198659/251‑1 (MQ=255)                                                                                                                                                                               cgacgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAAAGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGCGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGATTCGGCAGGGGGTg                                                     >  2:364611/1‑251 (MQ=255)                                                                                                                                                                                 acgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCg                                                   <  2:107733/251‑1 (MQ=255)                                                                                                                                                                                  cgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCgg                                                  <  2:288926/251‑1 (MQ=255)                                                                                                                                                                                       tccGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCATTCGCCACCGGCAGTTTCGGCCGGGGGTGCGgtggtt                                             >  2:291015/2‑248 (MQ=255)                                                                                                                                                                                             aTACACTTCGTTCCAGCGCAGCGCGTCTTTACACGCTGGCAGGCGGGCATCGTTATCAATCCCCGGGACTTCCATGGCGTCCATCTCGGCGCACTCGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCCCGCTTCGGAAGCAGTTGGCAGCTCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCCggggggggggcggtttcaccg                                       >  2:17098/1‑233 (MQ=255)                                                                                                                                                                                                 acTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTc                                   <  1:252502/251‑1 (MQ=255)                                                                                                                                                                                                        tCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCCTCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGGATGGTGCGCGCCCCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCCGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCCGCAGGTTCGGCAGGGAGGGCGGTGTTTTCCCCGTGTCggtac                              >  2:300820/1‑245 (MQ=255)                                                                                                                                                                                                             cgcagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAAc                       <  2:157564/251‑1 (MQ=255)                                                                                                                                                                                                              gcagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAAcc                      <  2:225419/251‑1 (MQ=255)                                                                                                                                                                                                                    cgTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGa                <  2:81785/251‑1 (MQ=255)                                                                                                                                                                                                                    cgTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCCGCGCGGATCCCCGCTTCGGAAGCCGTTGGCAGATCCGGGTGGGCTTTCCCCAGCGCATTCCCCACCCGCAGGTTCGGCAGGGGGGGCGGGGGTTTTACCGTGTTGATACAGTTAACCAgcagc                >  2:140279/1‑247 (MQ=255)                                                                                                                                                                                                                      tCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTTCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGAAGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTTGGCAGGGAGTGGGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACg              >  1:146977/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACg        <  1:68716/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATCCGCTACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGcc  <  2:189280/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggCAGGCGGGTATCGTTATCAATCACCGAGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATAGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGCGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGGTTAGGCAGGGAGTGCGGTGTTTTCACCGGGTCGATACAGTTAAACAGCAGACGGTAACGGTCGcc  >  1:318460/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                CTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCC  >  NC_000913/66665‑67140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑