Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,847	C→T	G497G (GGG→GGA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,847	0	C	T	100.0%	158.1 / NA	52	G497G (GGG→GGA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/29);  total (23/29)

AGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGG  >  NC_000913/66602‑67092                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTa                                                                                                                                                                                                                                                  <  2:75599/251‑1 (MQ=18)         ggCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACtt                                                                                                                                                                                                                                          <  2:53819/251‑1 (MQ=18)          gCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTc                                                                                                                                                                                                                                         >  1:198659/1‑251 (MQ=18)           ccTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCg                                                                                                                                                                                                                                        <  1:204879/251‑1 (MQ=18)              gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACGACTTAGCGACGAAATCCGTAATACACTTCGt                                                                                                                                                                                                                                       <  1:321896/249‑1 (MQ=18)                           ggCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGt                                                                                                                                                                                                                        <  2:289854/250‑1 (MQ=18)                           aGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGt                                                                                                                                                                                                                        >  1:317632/1‑251 (MQ=18)                              gTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGACCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGATTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACCCCTGATGCGCCTACTTATCGACGAAATCCGTAATCCACTTCGTTCCAGCGCCGCGCGTCtt                                                                                                                                                                                                                     >  2:335232/1‑251 (MQ=18)                                 gCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGTGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTaa                                                                                                                                                                                                                  <  1:125360/251‑1 (MQ=18)                                   aTCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAAc                                                                                                                                                                                                                <  2:105734/251‑1 (MQ=18)                                                                cTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCAc                                                                                                                                                                                   >  1:197409/1‑251 (MQ=21)                                                                  tATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCAc                                                                                                                                                                                   <  2:193167/249‑1 (MQ=21)                                                                              aaaaTCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGt                                                                                                                                                                     <  2:371042/251‑1 (MQ=25)                                                                                          cgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATGCACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCgg                                                                                                                                                         <  2:161705/251‑1 (MQ=25)                                                                                                ccTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaact                                                                                                                                                   <  1:25530/251‑3 (MQ=25)                                                                                                 cTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCCTCTCGGCGaactg                                                                                                                                                  >  1:178710/1‑248 (MQ=25)                                                                                                   gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCCTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGaactg                                                                                                                                                  <  1:36315/249‑4 (MQ=25)                                                                                                          cgcgCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACGTCGTTCCAGCGCAGCTCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATTGCGGGCAGCTCGGCGAACTGGCGCATATc                                                                                                                                         <  2:162281/251‑1 (MQ=21)                                                                                                             gcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCCCGGCGAACTGGCGCATATCGtt                                                                                                                                      >  2:68716/1‑251 (MQ=25)                                                                                                               cAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATTACCGTGAGTTCCATGTCGTGCATCTCGGCGAACTGGCGCCTATCGTTgg                                                                                                                                    >  2:62768/1‑250 (MQ=25)                                                                                                                   gTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGCATCGTTAACAATCACCGTGATTTCAAAGGCGTGCATCCCGGCGAAATGGCGCATATCGTTGAGGtt                                                                                                                                >  1:355072/1‑251 (MQ=25)                                                                                                                             gCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATgg                                                                                                                      <  2:70166/251‑1 (MQ=37)                                                                                                                               gTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCCTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCt                                                                                                                    >  1:269995/1‑251 (MQ=37)                                                                                                                                        cGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACggg                                                                                                           >  1:204129/1‑250 (MQ=37)                                                                                                                                           aTGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGTGATTTCAATGTCGTGCATCTCGGCCAACTGGCGCATATCGTTTAGGTTCAGCGCATGGCTGGAGACGGTAtt                                                                                                        >  2:80104/1‑250 (MQ=37)                                                                                                                                                        aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGccacca                                                                                           <  2:141922/251‑1 (MQ=38)                                                                                                                                                        aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGCTATCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCCGCGCATGGCTTAAGACGGTATGGTGCGCGccaccc                                                                                           >  2:286133/1‑250 (MQ=38)                                                                                                                                                           cGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCg                                                                                        >  1:275643/1‑251 (MQ=38)                                                                                                                                                              ccTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATTTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGTGCCACCAGCGAgg                                                                                     <  1:297066/251‑1 (MQ=255)                                                                                                                                                                 aCAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATc                                                                                  >  1:81785/1‑251 (MQ=255)                                                                                                                                                                         gCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTc                                                                          <  1:222615/251‑1 (MQ=255)                                                                                                                                                                          cAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCg                                                                         >  2:242697/1‑251 (MQ=255)                                                                                                                                                                              gtgtAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCCCCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATAGTGCGCGCCACCCGCCGGGATCCACGCCTCGGacg                                                                     >  2:85525/1‑249 (MQ=255)                                                                                                                                                                                  aGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGt                                                                 <  2:21175/251‑1 (MQ=255)                                                                                                                                                                                   ggCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGGATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGtt                                                                >  1:1843/1‑251 (MQ=255)                                                                                                                                                                                            aGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATcc                                                       <  2:4922/251‑1 (MQ=255)                                                                                                                                                                                                gcgTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCTGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCTTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGtt                                                   <  1:240492/251‑1 (MQ=255)                                                                                                                                                                                                        gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTc                                           >  1:321333/1‑251 (MQ=255)                                                                                                                                                                                                        gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTc                                           <  1:291915/251‑1 (MQ=255)                                                                                                                                                                                                              tCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGGATTGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGATGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCCCCAGCGAGGATCCACGCCTCGGAAGCAGTTGGCCGATCCGGTTGCGCTTTCCACAgc                                     >  1:249174/1‑251 (MQ=255)                                                                                                                                                                                                                aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGTGATTTCAATGTCGTGCATCTCCGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGCTGCGCTTTCCACAgcgc                                   >  1:154949/1‑251 (MQ=255)                                                                                                                                                                                                                aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCTCTTTCCACAgcgc                                   <  1:253757/251‑1 (MQ=255)                                                                                                                                                                                                                aGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACAgcgc                                   >  2:61976/1‑251 (MQ=255)                                                                                                                                                                                                                  gctttACACACCTGATGCGACTACTGAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                 <  2:222758/248‑1 (MQ=255)                                                                                                                                                                                                                         acacctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACCGCGCATTCGCCAc                          >  1:102596/7‑251 (MQ=255)                                                                                                                                                                                                                           ccctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCg                        <  2:215944/247‑1 (MQ=255)                                                                                                                                                                                                                                  gcgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGttt                 <  1:10787/247‑1 (MQ=255)                                                                                                                                                                                                                                   cgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCTCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCGTTCCACAGCGCATTCGCCACCGGCAGTTTc                <  2:233259/248‑1 (MQ=255)                                                                                                                                                                                                                                             gCGACGAAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGt      <  2:198659/251‑1 (MQ=255)                                                                                                                                                                                                                                              cgacgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAAAGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGCGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGATTCGGCAGGGGGTg     >  2:364611/1‑251 (MQ=255)                                                                                                                                                                                                                                                acgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCg   <  2:107733/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cgaAATCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCgg  <  2:288926/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGG  >  NC_000913/66602‑67092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑