Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,561,368	C→T	P147S (CCG→TCG)	kptA →	RNA 2'‑phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,561,368	0	C	T	100.0%	78.5 / NA	23	P147S (CCG→TCG)	kptA	RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

ATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCT  >  NC_000913/4561153‑4561610                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   aTGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCg                                                                                                                                                                                                                 >  1:21789/1‑251 (MQ=255)      aGGTGAATCCGCGCGGTACCGGGGAATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGGGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACCGGAAATGGCGAAACg                                                                                                                                                                                                            >  2:220082/1‑251 (MQ=255)              ccGCGCGGTTCGGGGGCATGGGCCTTCGCAGGGCGTTATTTCCTTTGCCAAAAAAACACCGCCACAGTTGCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTcc                                                                                                                                                                                                    <  2:331801/251‑1 (MQ=255)                    ggtgcgggggCATTCGACTGCGGAGGTCGCTATTTCCTTTGCGAAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTAGTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCt                                                                                                                                                                                              <  2:17798/245‑1 (MQ=255)                         aGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCa                                                                                                                                                                                          >  1:271634/1‑250 (MQ=255)                                  cGACTTCGCAGGTCGCTATTTCTTTTGACGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAAc                                                                                                                                                                                <  2:298302/251‑1 (MQ=255)                                               cGCTATTTCTTTGGCCAAAAAAACCCCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGa                                                                                                                                                                   <  2:140169/251‑1 (MQ=255)                                                                               aGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCGCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTgg                                                                                                                                   <  2:116337/251‑1 (MQ=255)                                                                                gTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGt                                                                                                                                  >  2:71498/1‑251 (MQ=255)                                                                                    ctctACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCATTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAgg                                                                                                                              >  1:114753/1‑251 (MQ=255)                                                                                        aCCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGTCTCCCTTTCTGGCAAGCGGAAAACGGGTTCTGGCTGACAGCAACGGTTGCGGTTGATTTTCTTGAGGGGTAAGGGa                                                                                                                            <  2:177850/249‑1 (MQ=255)                                                                                                           tttCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGAcc                                                                                                       >  2:33090/1‑251 (MQ=255)                                                                                                                        aTAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTa                                                                                           >  1:149826/1‑250 (MQ=255)                                                                                                                                 cAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa                                                                                 <  1:71498/251‑1 (MQ=255)                                                                                                                                    gggCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACtg                                                                              <  2:15096/251‑1 (MQ=255)                                                                                                                                          aTTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTg                                                                         >  2:64147/1‑250 (MQ=255)                                                                                                                                             gCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATa                                                                      <  1:58891/250‑1 (MQ=255)                                                                                                                                              cAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAATGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAg                                                                    <  2:248436/251‑1 (MQ=255)                                                                                                                                                 gTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAgcgc                                                                 >  2:155242/1‑251 (MQ=255)                                                                                                                                                                       ctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATCAACAACGCcgccgcg                                           >  2:43944/1‑251 (MQ=255)                                                                                                                                                                           gctgATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACTGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTGTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCcgccgcgcc                                         <  2:227608/249‑1 (MQ=255)                                                                                                                                                                                                 aaGTGGGAGCAAGACACGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGACGCTGGCAAgcgc                 >  2:95258/1‑251 (MQ=255)                                                                                                                                                                                                                acGGATCGTCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCt  <  1:212232/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   ATGGTAGGTGCATCCGCGCGGTTCAGGGGCATTCGACTTCGCAGGTCGCTATTTCCTTTGCCGAAAAAACACCGCCACAGTTTCTCTACCACGGTACGGCAAGCCGTTTCCTTGACGAGATAAAAAAACAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCT  >  NC_000913/4561153‑4561610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑