Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,486	C→T	intergenic (‑166/‑88)	yjiC ← / → ytiC	DUF2686 domain‑containing protein YjiC/protein YtiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,486	0	C	T	100.0%	19.0 / NA	7	intergenic (‑166/‑88)	yjiC/ytiC	DUF2686 domain‑containing protein YjiC/protein YtiC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

GTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGCTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACTAATTGGAATTTGATGTTGCTATATTGAGGTCTATATTAATAATGCCTGTGAATGGTATTTTTGATGTATTTGATATGTTATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGC  >  NC_000913/4556243‑4556658                                                                                                                                                                                                                                                    |                                                                                                                                                                             gTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCAttt                                                                                                                                                                        >  1:86021/1‑250 (MQ=255)                                        aatTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTCTTTGACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACt                                                                                                                                >  2:201246/1‑251 (MQ=255)                                                                                                aGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACTAATTGGAATTTGATGTTGCTATATTGAGGTCTATATTAATAATGCCTGTGAATGGt                                                                        <  2:285708/251‑1 (MQ=255)                                                                                                                                             aaaaaGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCAt                                                                                                                                                                          >  1:58029/1‑108 (MQ=255)                                                                                                                                             aaaaaGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCAt                                                                                                                                                                          <  2:58029/108‑1 (MQ=255)                                                                                                                                                    gaAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACTAATTGGAATTTGATGTTGCTATATTGAGGTCTATATTAATAATGCCTGTGAATGGTATTTTTGATGTATTTGATATGTTATCAATTTATATTATTTACAAACTAATTg                    <  2:86021/251‑1 (MQ=255)                                                                                                                                                                      gcatgTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGTTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACTAATTGGAATTTGATGTTGCTATATTGAGGTCTATATTAATAATGCCTGTGAATGGTATTTTTGATGTATTTGATATGTTATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGc  <  1:158399/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                             GTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTAAAGTATCAAAAAACGCTCATTTAAAATTATTTGCATGCAATTTAAAAGCATATCTTATTACTAATTGGAATTTGATGTTGCTATATTGAGGTCTATATTAATAATGCCTGTGAATGGTATTTTTGATGTATTTGATATGTTATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGC  >  NC_000913/4556243‑4556658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑