Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,498	A→G	R12R (CGT→CGC)	pyrL ←	pyrBI operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,498	0	A	G	100.0%	79.1 / NA	24	R12R (CGT→CGC)	pyrL	pyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/10);  total (14/10)

ACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGC  >  NC_000913/4472252‑4472739                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  aCAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGcgcg                                                                                                                                                                                                                                               >  1:132806/1‑251 (MQ=255)         cAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGAGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGAc                                                                                                                                                                                                                                        >  2:315393/1‑250 (MQ=255)             gACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAAt                                                                                                                                                                                                                                   <  1:50496/251‑1 (MQ=255)                                    gTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAACTGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACAt                                                                                                                                                                                                            >  1:364568/1‑251 (MQ=255)                                      tGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc                                                                                                                                                                                                          >  1:111059/1‑251 (MQ=255)                                                                 gTCGCCAGCACCAGATGAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATtata                                                                                                                                                                                 <  2:78023/249‑1 (MQ=255)                                                                     ccAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATc                                                                                                                                                                           <  1:232999/251‑1 (MQ=255)                                                                                       cctggcggataaagTCGTTTAATGGAAATAATATGTTCCT‑ATATTAG‑GGGTTTGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCCCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc                                                                                                                                                         <  1:259825/238‑1 (MQ=255)                                                                                          cgcgACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAtt                                                                                                                                                      >  2:118512/1‑251 (MQ=255)                                                                                            cgACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg                                                                                                                                                    <  1:162366/251‑1 (MQ=255)                                                                                                      cGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCgg                                                                                                                                                                                        >  2:39970/1‑205 (MQ=255)                                                                                                      cGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAAGGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCgg                                                                                                                                                                                        <  1:39970/205‑1 (MQ=255)                                                                                                         ttA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACGCAGCGTCAGAAGCGGCACAAGCATTTGATGCAATAtttt                                                                                                                                        <  1:20791/250‑1 (MQ=255)                                                                                                                    gagatGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCACTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCAGACATCCTCCCGGCAAATTGTCCGTCATTATACTCATCGTCAGAAGCGGCGCAAGCATGTGATACAAGATTTTGTCAgagaaa                                                                                                                              <  2:34261/247‑6 (MQ=255)                                                                                                                     atatGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc                                                                                                                            <  2:132806/251‑1 (MQ=255)                                                                                                                       atGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCCTTTGATGCAATATTTTGTCCGCGCAAACgg                                                                                                                          >  2:17708/1‑251 (MQ=255)                                                                                                                       atGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg                                                                                                                          >  1:177591/1‑251 (MQ=255)                                                                                                                                   t‑aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt                                                                                                              >  1:79256/1‑251 (MQ=255)                                                                                                                                                      ttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAg                                                                                                                                                                                                                                          >  1:58361/1‑109 (MQ=255)                                                                                                                                                      ttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAg                                                                                                                                                                                                                                          <  2:58361/109‑1 (MQ=255)                                                                                                                                                                                                     gggAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCa                                              >  1:75818/1‑250 (MQ=255)                                                                                                                                                                                                                        gaagaaAAACGGCAGGACAGCGTCTTTTTTCAGGCGAGGTAAGACAAAATGCCGCACACACTGAACCATACCTCCTCCCGGCAAATTGTCCGGCATTATACTCACCGACAGACGCGGCGCAAGCATTTGATGCAATATTTTGTCCGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTg                          >  1:232682/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttttttCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGc  >  1:160849/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttttttCAGGCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGc  >  1:364450/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  ACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTA‑TGGAAATGATATGTTTCTGATAT‑AGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGC  >  NC_000913/4472252‑4472739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑