Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,379,954	A→G	G188G (GGT→GGC)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,379,954	0	A	G	100.0%	203.2 / NA	59	G188G (GGT→GGC)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/29);  total (30/29)

AACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGT  >  NC_000913/4379708‑4380196                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aaCGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGt                                                                                                                                                                                                                                                >  1:67430/1‑251 (MQ=255) aaCGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGt                                                                                                                                                                                                                                                <  1:197586/251‑1 (MQ=255)     gCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTc                                                                                                                                                                                                                                            >  1:56987/1‑251 (MQ=255)              tCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATc                                                                                                                                                                                                                                   >  2:269342/1‑251 (MQ=255)                caccaGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTCGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCtt                                                                                                                                                                                                                                 >  1:212461/1‑251 (MQ=255)                 cccaGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTc                                                                                                                                                                                                                                <  1:229833/250‑1 (MQ=255)                   caGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCATCTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGt                                                                                                                                                                                                                              <  2:106963/251‑1 (MQ=255)                                    ccGTTCATACGGTTTGCGTTTAGGCGTCAGGTTTCACCCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCGTTCGAACTTTCGTCTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCGCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAGCGATGCGCCAGc                                                                                                                                                                                                             <  2:130809/251‑1 (MQ=255)                                     cGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCg                                                                                                                                                                                                            >  1:256104/1‑251 (MQ=255)                                      gTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGt                                                                                                                                                                                                           >  1:90999/1‑251 (MQ=255)                                         cATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAAt                                                                                                                                                                                                        <  2:305598/251‑1 (MQ=255)                                            aCGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATgg                                                                                                                                                                                                      <  1:99829/250‑1 (MQ=255)                                             cGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCa                                                                                                                                                                                                    <  1:349928/251‑1 (MQ=255)                                               gTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGc                                                                                                                                                                                                  <  1:354532/251‑1 (MQ=255)                                                    cGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGAc                                                                                                                                                                                             >  1:68150/1‑251 (MQ=255)                                                     gTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGAcc                                                                                                                                                                                            >  1:91531/1‑251 (MQ=255)                                                         aGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATg                                                                                                                                                                                        >  2:210768/1‑251 (MQ=255)                                                                  gTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTgg                                                                                                                                                                                <  2:358541/250‑1 (MQ=255)                                                                              aGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAAc                                                                                                                                                                   >  2:275410/1‑251 (MQ=255)                                                                               gCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACt                                                                                                                                                                  <  1:175913/251‑1 (MQ=255)                                                                                        cAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAAGGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCAc                                                                                                                                                          <  1:240437/250‑1 (MQ=255)                                                                                         aGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCAcgc                                                                                                                                                        >  2:311330/1‑251 (MQ=255)                                                                                           ggTCGCGATAACAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCAGCAGCCGGATCGACGTGTTTCAGGCAGACTTCGGAGCAGTCGCCCACGATAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCACACGCTCCTTCTTGCCGCGGTCGCGGCTATCCTCGCTATAACGCGGCGCCCGCGTAATGGCAGCCGGCCCGATGAAATCTGGGTCAAGGCCAAACTACGCGCACgcgg                                                                                                                                                      >  2:374153/1‑251 (MQ=255)                                                                                               gcgATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGt                                                                                                                                                   >  2:60237/1‑250 (MQ=255)                                                                                                   tAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGcac                                                                                                                                              >  1:222832/1‑251 (MQ=255)                                                                                                       aaaGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAAc                                                                                                                                          >  1:356687/1‑251 (MQ=255)                                                                                                           tCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCCCAAACcac                                                                                                                                       >  2:353979/1‑250 (MQ=255)                                                                                                                 gAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGAt                                                                                                                                <  1:103405/251‑1 (MQ=255)                                                                                                                  aaCTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGCGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATg                                                                                                                               >  1:232742/1‑251 (MQ=255)                                                                                                                         tACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAAcc                                                                                                                                         >  2:119562/1‑234 (MQ=255)                                                                                                                         tACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAAcc                                                                                                                                         <  1:119562/234‑1 (MQ=255)                                                                                                                         tACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCgg                                                                                                                        >  2:93926/1‑251 (MQ=255)                                                                                                                                                    cGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTgcg                                                                                             <  2:339566/251‑1 (MQ=255)                                                                                                                                                       aTCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCCCAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCg                                                                                          >  1:40102/1‑251 (MQ=255)                                                                                                                                                       aTCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAc                                                                                                                           >  1:314455/1‑218 (MQ=255)                                                                                                                                                       aTCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAc                                                                                                                           <  2:314455/218‑1 (MQ=255)                                                                                                                                                                   cGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCAAGCGTAATGGCAGCCGGACAGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGAAACCGGAGAACTGGGTATACTTCGCCAGCTGCGCCGGGGTCTGGATGt                                                                              >  1:226560/1‑251 (MQ=255)                                                                                                                                                                             tCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTAccc                                                                      <  2:69812/249‑1 (MQ=255)                                                                                                                                                                             tCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTAccc                                                                      <  2:301468/249‑1 (MQ=255)                                                                                                                                                                               ggAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAcc                                                                                                                          <  2:248831/195‑1 (MQ=255)                                                                                                                                                                               ggAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAcc                                                                                                                          >  1:248831/1‑195 (MQ=255)                                                                                                                                                                                    aGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcgcg                                                             <  1:269342/251‑1 (MQ=255)                                                                                                                                                                                    aGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCAGCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCcgcg                                                             <  1:161431/251‑1 (MQ=255)                                                                                                                                                                                       aGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTg                                                          <  1:60237/251‑1 (MQ=255)                                                                                                                                                                                               aaaGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGtt                                                  >  1:128379/1‑251 (MQ=255)                                                                                                                                                                                               aaaGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACGGGTGATACTTCGCCATCTGCGCCGGGGGCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGtt                                                  <  2:225172/251‑1 (MQ=255)                                                                                                                                                                                                 aGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAAACGGAGAACTGGTGATACGTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGc                                                >  2:132647/1‑251 (MQ=255)                                                                                                                                                                                                          ccATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCgatgat                                         <  2:356687/249‑1 (MQ=255)                                                                                                                                                                                                             tACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACg                                    >  2:18809/1‑251 (MQ=255)                                                                                                                                                                                                                    ttCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGGCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGAt                             >  1:26617/1‑251 (MQ=255)                                                                                                                                                                                                                         gCTGTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCtt                        <  1:283331/251‑1 (MQ=255)                                                                                                                                                                                                                            gTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTcc                      <  1:251974/250‑1 (MQ=255)                                                                                                                                                                                                                            gTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTcc                      >  2:353428/1‑250 (MQ=255)                                                                                                                                                                                                                            gTTCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTcc                      <  1:311330/250‑1 (MQ=255)                                                                                                                                                                                                                             ttCAACTGCGCCATACGCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTcc                      >  2:338493/1‑249 (MQ=255)                                                                                                                                                                                                                                    gcgcCATACGCTCCTGCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCg             <  2:220540/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccATACGCTCCTGCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTGCGCCATCTGCGCCGGGGTCTGGATGTTAGTTCCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATg          <  2:197759/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCTCCTTCTTGCCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTgg    >  1:188309/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccTTCTTGCCGTGGTCGCGGCTATCGTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGCTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTGTGATCGCTTCCAGACTTGCGATGAAGTGGGt  <  2:191210/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   AACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGCAGTAGCCCACGAAAGTACAGCTCCATACGCCGTTCTGGCTGTTCAACTGCGCCATACGCTCCTTCTTACCGTGGTCGCGGCTATCTTCGTTATAACGATGCGCCAGCGTAATGGCAGCCGGACCGATGAACTCTGGGTTCAGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGT  >  NC_000913/4379708‑4380196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑