Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,308,047	T→C	Q144Q (CAA→CAG)	alsE ←	D‑allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,308,047	0	T	C	100.0%	88.7 / NA	28	Q144Q (CAA→CAG)	alsE	D‑allulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

CTTTTTGCAGCCAGAATCTGCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGTTGAGCAATGTAATCCTGCGGCCGCGTCACCATC  >  NC_000913/4307814‑4308287                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 cTTTTTGCAGCCAGAATCTGCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTAATCCAGACCTTCTCGGTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCggg                                                                                                                                                                                                                                 >  1:346754/1‑251 (MQ=255)           ccAGAATCTGCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                                                                                                                                       >  1:311060/1‑251 (MQ=255)              gAATCTTCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                                                                                                                                    <  1:217934/251‑1 (MQ=255)               aaTCTGCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGTCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGAAAGTCATg                                                                                                                                                                                                                    >  2:83129/1‑250 (MQ=255)               aaTCTGCGCGGTCATAATTATCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                                                                                                                                    >  1:207986/1‑250 (MQ=255)                    ggtcgGTCTTAATTTTCCATGCTTAGTCGATATTTTCCGCAGGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTa                                                                                                                                                                                                              <  2:206149/248‑1 (MQ=255)                    gcgcGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGt                                                                                                                                                                                                               >  1:185511/1‑250 (MQ=255)                        ggTCATAATTCTACATGCCTCGCCGGTCTTTTCCGCATGGTTAAACAGGCCGGCTGAACCGACGACAAAGACATCCGCCCCTGCCGCCATCCGTCTTTCGTAAGCTGCCTGGTTGCATGAACCGTCCACCTCAATTTCGTGCTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGACAAGTTTATCCAGCATTTCCGGAATGACCGGCTTCCCGGCAAAGCCGGGATCGCCGGTCATGCCAGTAAttt                                                                                                                                                                                                          >  2:253075/1‑251 (MQ=255)                          tCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                                                                                                                                         >  2:309433/1‑250 (MQ=255)                           aaTAATTCTCCAGGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGCCATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt                                                                                                                                                                                                       <  2:45554/250‑1 (MQ=255)                               aTTCCCCATGCTGCGTCGATATTTTCCGCATGATTAAACAGGCCGTAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGc                                                                                                                                                                                                   <  2:106531/251‑1 (MQ=255)                                       tGCTTCGTCGATCTTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                                                                                                                                            >  2:12370/1‑250 (MQ=255)                                          ttCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGatat                                                                                                                                                                                        >  1:257008/1‑251 (MQ=255)                                           tcgtcgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTGATCGGCCTTATGGata                                                                                                                                                                                         <  1:309771/249‑1 (MQ=255)                                                      ttCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATg                                                                                                                                                                             <  1:309433/250‑1 (MQ=255)                                                                                       gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                                                                                                                                           <  1:111146/251‑1 (MQ=255)                                                                                          aaaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcccc                                                                                                                                         >  1:65453/1‑250 (MQ=255)                                                                                                          gccgccATCAGTTTTTCGTAAGTGGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTGCTCGTTCACGCCATGCCGTCAGTTCGGCAAGTTTAGCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTAGTGATATAGTATTTCATTGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTGTCATGGCATGAc                                                                                                                         <  2:176134/250‑1 (MQ=255)                                                                                                                     tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTtcat                                                                                                             >  1:258378/1‑251 (MQ=255)                                                                                                                                   ccTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAAc                                                                                               <  1:177635/251‑1 (MQ=255)                                                                                                                                                                    aCTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGtg                                                              <  1:198018/251‑1 (MQ=255)                                                                                                                                                                                  cGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGACCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTcc                                                <  2:257008/251‑1 (MQ=255)                                                                                                                                                                                                         ggCAAGTTTATCCAGCATTTCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATGTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAGTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGGTGAGCAATGt                         <  2:45622/251‑1 (MQ=255)                                                                                                                                                                                                                           ttCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca                                                                                                                             >  1:30428/1‑133 (MQ=255)                                                                                                                                                                                                                           ttCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca                                                                                                                             <  2:30428/133‑1 (MQ=255)                                                                                                                                                                                                                           ttCAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGTTGAGCAATGTAATCCTGCGGCCGCGTc        <  2:217653/250‑1 (MQ=255)                                                                                                                                                                                                                             cAGGAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGTTGAGCAATGTAATCCTGCGGCCGCGTCAc      <  2:104369/250‑1 (MQ=255)                                                                                                                                                                                                                                gAATGAACGGCTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGTTGAGCAATGTAATCCTGCGGCCGCGTCACCATc  >  1:288362/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 CTTTTTGCAGCCAGAATCTGCGCGGTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCCAGTTGAGCAATGTAATCCTGCGGCCGCGTCACCATC  >  NC_000913/4307814‑4308287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑