Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,303,646	T→C	D155G (GAC→GGC)	mdtN ←	putative multidrug efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,303,646	0	T	C	100.0%	106.5 / NA	33	D155G (GAC→GGC)	mdtN	putative multidrug efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

AGGGCGCATGGCAGAAGCAAATTGCCCGACGGAGGTTTTGAGGGAAATGACCCGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCG  >  NC_000913/4303405‑4303891                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aGGGCGCATGGCAGAAGCAAATTGCCCGACGGAGGTTTTGAGGGAAATGACCCGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCt                                                                                                                                                                                                                                               <  1:12135/250‑1 (MQ=255)                         cccGACGGAGTTTTTGAGGGAAAGGACCGGGCCATAAAACGGCGCGCGAACGGTCGCCATTTCCGGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCa                                                                                                                                                                                                                      <  2:357249/251‑1 (MQ=255)                          ccGACGGAGGTTTTGAGGGAAATGACCCGGCCATCAAACGGCGCGCGAACGGTCGCCCTTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACa                                                                                                                                                                                                                                                  >  1:16891/1‑222 (MQ=255)                          ccGACGGAGGTTTTGAGGGAAATGACCCGGCAATCAAACGGCGCGCGAACGGTCGCCATTTCCAGAGGCAGTTTGGTCAGGGCAATATCCGCGTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACa                                                                                                                                                                                                                                                  <  2:16891/222‑1 (MQ=255)                                 aGGTTTTGAGGGAAATGACCCGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGc                                                                                                                                                                                                              >  1:372972/1‑251 (MQ=255)                                         aGGGAAATGCCCCGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTgcg                                                                                                                                                                                                      <  1:80518/251‑1 (MQ=255)                                          gagAAATGACCCGGCCAGCAAACGGCGCGTGAGCGGTCGCGATTTCCATATGCAGTTGGGTCAGGGCAAGATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTGCAGTAATGGCTCGGTgcgg                                                                                                                                                                                                     <  2:245335/249‑1 (MQ=255)                                                   cccGGCCATCAAACGGCACGCGAACGGTCGCCATTTCCAGATGCCGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATgc                                                                                                                                                                                             >  2:73271/1‑249 (MQ=255)                                                    ccGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTAt                                                                                                                                                                                           >  1:243420/1‑251 (MQ=255)                                                        ccATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCtgt                                                                                                                                                                                       >  2:237990/1‑251 (MQ=255)                                                          aTCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTgg                                                                                                                                                                                     <  1:95071/251‑1 (MQ=255)                                                                     cgcgAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTcgcg                                                                                                                                                                          >  1:15317/1‑251 (MQ=255)                                                                          aCGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATTTATCTGTGGCCTTGTTCGCggcggc                                                                                                                                                                     >  2:205785/1‑251 (MQ=255)                                                                             gtggCCATTTCCAGAGGGAGTTGGGTCAGGGCAATTCCCGCTTCGGCCGCCGCGCGCTGGGCAACAAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGGTTCTGCGGCGCTCTGCGCCGTTCGTGCACGGCCAACAGCTTTCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGGGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACg                                                                                                                                                                  <  2:371831/248‑1 (MQ=255)                                                                                       ccAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCt                                                                                                                                                        >  1:230342/1‑251 (MQ=255)                                                                                            tGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGt                                                                                                                                                   >  1:249579/1‑251 (MQ=255)                                                                                                   ggtgcAGGGAAAAATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCGGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTa                                                                                                                                            <  2:49583/247‑1 (MQ=255)                                                                                                    ggTCAGGGACATATCCGCTTCGACCGCCGCACGCTGGGCAGCTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTa                                                                                                                                            <  1:258643/250‑1 (MQ=255)                                                                                                         gggCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATTCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACaaa                                                                                                                                                                                                                                 <  1:346781/160‑1 (MQ=255)                                                                                                         gggCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACaaa                                                                                                                                                                                                                                 >  2:346781/1‑160 (MQ=255)                                                                                                                    gCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGccccca                                                                                                                            >  2:175279/1‑245 (MQ=255)                                                                                                                         gACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGtt                                                                                                                      >  1:79618/1‑251 (MQ=255)                                                                                                                                gcACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGATGGCCGCTGACTGAGCCTGTAACAATACGGCATTAAGATCAGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAACACCTTCTTTCAGTAATGGCTCTGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGAGGAACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTc                                                                                                               >  1:215005/1‑251 (MQ=255)                                                                                                                                           aaCTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAacgctacgct                                                                                                    <  1:9492/251‑1 (MQ=255)                                                                                                                                                 ggCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAAGACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGa                                                                                              <  2:92003/250‑1 (MQ=255)                                                                                                                                                          gccgcTGACGGCGCTTGCGGCTGACGTCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTTCGCCGTTCTTTCACGGCCAACATCCTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCAcc                                                                                                                              <  2:66055/210‑1 (MQ=255)                                                                                                                                                          gccgcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCGTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTGGCGCGTCAACGCTACGCTGGGTGAGCATAAttt                                                                                     <  2:166889/251‑1 (MQ=255)                                                                                                                                                          gccgcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCCTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGGGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCCCGGGCTTTTTTTACCGTGGCATTAACCGAGTCGGCAcc                                                                                                                              >  1:66055/1‑210 (MQ=255)                                                                                                                                                                                            aaCAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCtt                                                    <  1:259263/250‑1 (MQ=255)                                                                                                                                                                                                aTACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGcc                                               >  2:69715/1‑251 (MQ=255)                                                                                                                                                                                                            gATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCg                                   >  2:177952/1‑251 (MQ=255)                                                                                                                                                                                                                       gcggcgCGCTGCGCCGTTCTTGCACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGcgcg                         <  2:230342/250‑1 (MQ=255)                                                                                                                                                                                                                                             cACGGCCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTCACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCg  >  1:308897/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AGGGCGCATGGCAGAAGCAAATTGCCCGACGGAGGTTTTGAGGGAAATGACCCGGCCATCAAACGGCGCGCGAACGGTCGCCATTTCCAGATGCAGTTTGGTCAGGGCAATATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCG  >  NC_000913/4303405‑4303891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑