Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,129,790	T→C	I373I (ATT→ATC)	metB →	O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,129,790	0	T	C	100.0%	155.0 / NA	46	I373I (ATT→ATC)	metB	O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

CAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCC  >  NC_000913/4129545‑4130027                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              cAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCg                                                                                                                                                                                                                                           >  2:301789/1‑250 (MQ=255)    cAAAAAGGCTTTGGCGCCATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGCGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCt                                                                                                                                                                                                                                       >  2:57680/1‑251 (MQ=255)    cAAAAAGGCTTGGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCt                                                                                                                                                                                                                                       <  2:64070/251‑1 (MQ=255)     aaaaaGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCGGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTGAATCGCCGACCTg                                                                                                                                                                                                                                      <  2:163118/251‑1 (MQ=255)        aaGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGa                                                                                                                                                                                                                                    >  1:349693/1‑250 (MQ=255)         aGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCTTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAACCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTGAAGCGCCGACCTGGaaa                                                                                                                                                                                                                                  <  2:55082/251‑1 (MQ=255)           gCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAAt                                                                                                                                                                                                                                >  1:255430/1‑251 (MQ=255)                 gcgcAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTc                                                                                                                                                                                                                          >  2:355042/1‑251 (MQ=255)                    cAATGTTGCGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGAACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCgg                                                                                                                                                                                                                       >  1:134957/1‑251 (MQ=255)                    cAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCt                                                                                                                                                                                                                            <  1:24855/246‑1 (MQ=255)                    cAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCt                                                                                                                                                                                                                            >  2:24855/1‑246 (MQ=255)                     aaTGTTGAGTTTTGAACTGGATGGGGATGAGCAGACGCTGCGTCGTTTCCTGTGCGGGCTGTCGTTGTTTACGCTGGTGGAATCATTAGTGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGTCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCGGGAAAATGGCTTCCggg                                                                                                                                                                                                                      <  2:80919/251‑1 (MQ=255)                         ttGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGACTCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGc                                                                                                                                                                                                                  >  2:312475/1‑251 (MQ=255)                                  gAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAggg                                                                                                                                                                                                         >  2:269632/1‑251 (MQ=255)                                        gATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAGCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTaaaa                                                                                                                                                                                                   <  2:224069/251‑1 (MQ=255)                                         aTGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTaaaaa                                                                                                                                                                                                  <  1:269632/251‑1 (MQ=255)                                                             cgtcgtTTCCTGGGCGGGCTGTCGTGGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCgcaggca                                                                                                                                                                              <  1:54542/251‑1 (MQ=255)                                                                cgtTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGt                                                                                                                                                                                                       <  1:228032/223‑1 (MQ=255)                                                                cgtTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGt                                                                                                                                                                                                       >  2:228032/1‑223 (MQ=255)                                                                           ggggcTGTCGTTGTTTACGCTGGCGGAAGCATTAGGGGGAGTGAAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGTCGAAAGgtcg                                                                                                                                                                <  2:228984/250‑1 (MQ=255)                                                                                tGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGCCACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAACTGGCTTCCGGCTTGCAAACAAGGGGTAAACATGAGTGGGATTGCGCAGGCAGGGGCGAAAGgtcgtacgg                                                                                                                                                           >  2:14912/1‑247 (MQ=255)                                                                                   cGTTGTTTACGCCGGCGGAATCACTAGGGGGAGTGGAAAGTTTACTCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACATGGCAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGc                                                                                                                                                        >  2:231864/1‑251 (MQ=255)                                                                                    gttgttTACGCTGGCGGAATCATTAGGGGGAGTGGCAAGTTTAATCTCTCACGCAGCACCCATCACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGACGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGACGTCAGATGc                                                                                                                                                        >  2:55373/1‑250 (MQ=255)                                                                                       gttTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTg                                                                                                                                                                                      >  1:65444/1‑217 (MQ=255)                                                                                       gttTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTg                                                                                                                                                                                      <  2:65444/217‑1 (MQ=255)                                                                                                     aaTCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCagt                                                                                                                                       >  1:317424/1‑250 (MQ=255)                                                                                                           tAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTg                                                                                                                                 <  2:356761/250‑1 (MQ=255)                                                                                                                ggAGTGGAAAGTGTAATCTCCCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGAt                                                                                                                           <  2:305431/251‑1 (MQ=255)                                                                                                                      gAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAg                                                                                                                     <  1:315538/251‑1 (MQ=255)                                                                                                                       aaaGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAACAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGACGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAgt                                                                                                                    >  2:88135/1‑251 (MQ=255)                                                                                                                                 ctctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCg                                                                                                          >  2:202096/1‑251 (MQ=255)                                                                                                                                  tctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCgt                                                                                                         >  2:54559/1‑251 (MQ=255)                                                                                                                                   ctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCgtg                                                                                                        >  1:248112/1‑251 (MQ=255)                                                                                                                                     cACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTAGCTCCGCCGGTATTGAAGATGGCGAAGATGTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTTAAAATTAGTGTGATTGCGCAGGCAGGGTCGAAAGGTCTTCAGCTGCATAAATTTGGTGGCAGTAGGCTGGCTGATGTGAAGTGTTATTTGCGTGTc                                                                                                      <  1:43955/251‑1 (MQ=255)                                                                                                                                             aaCCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCAt                                                                                              <  1:185152/251‑1 (MQ=255)                                                                                                                                                      acaTGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGa                                                                                                                                                                                         >  2:289458/1‑151 (MQ=255)                                                                                                                                                      acaTGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGa                                                                                                                                                                                         <  1:289458/151‑1 (MQ=255)                                                                                                                                                                aTGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACtctc                                                                             >  1:136421/1‑249 (MQ=255)                                                                                                                                                                      ccAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAAAGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGAc                                                                     <  2:257445/251‑1 (MQ=255)                                                                                                                                                                         gAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGCGTACTCTCAGCCTGACGat                                                                  >  1:336698/1‑251 (MQ=255)                                                                                                                                                                                      ccgccgGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGttt                                                     >  1:314737/1‑251 (MQ=255)                                                                                                                                                                                              atatcCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTATGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGTAGTACTCTCAGCCTGACGATATGATGGTGGTTTccgccgcc                                             >  1:278892/4‑251 (MQ=255)                                                                                                                                                                                              aTCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGATGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTccgccgcc                                             >  1:278888/1‑251 (MQ=255)                                                                                                                                                                                                                       ccACCGGTATTGAAGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGAtt                     <  1:217367/250‑1 (MQ=255)                                                                                                                                                                                                                                    aGATGGCGAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACt       <  2:248112/251‑1 (MQ=255)                                                                                                                                                                                                                                           gAAGATTTAATCGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGcc  >  1:303630/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              CAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCC  >  NC_000913/4129545‑4130027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑