Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,096,880	T→C	D190G (GAC→GGC)	rhaB ←	rhamnulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,096,880	0	T	C	97.4%	116.6 / NA	39	D190G (GAC→GGC)	rhaB	rhamnulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (19/19);  minor base G (0/1);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCAT  >  NC_000913/4096640‑4097126                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ggCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCtattgat                                                                                                                                                                                                                                              <  2:89824/251‑1 (MQ=255)               gAAGCCCATCAATGACCAGGTGCCAGAAGCGAGATAAGCAGCACGTGCGCCGTTTACCGGCGAGGCGCTAACCGCGTTGGCGGGCTCCTGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCCGGCAAATCCCGTGACCTTTGGAC‑TGACCCGGATGACTCGGGCGACCAAACCAAGCTTTGTTTGCCCCGCTCCCCGCCAGGACCGACCCGTCCCAGTCGCCGCCCGTGATATTTACCAGCTGCg                                                                                                                                                                                                                                >  2:187126/1‑251 (MQ=255)                    ccagcAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTGTGTTGGCCCCGGTCCACGCCAGTAGCGACTCGTCCCAGGCGGCGCTATTGATATTGACCAGTTGCTTGGTc                                                                                                                                                                                                                           <  2:217514/247‑1 (MQ=255)                             aCCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGCGGCGTTg                                                                                                                                                                                                                  <  2:104778/251‑1 (MQ=255)                               cAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCCACGGCGACCGCTGGAATCTCCTTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATCTTGACCAGTTGCGTGGTCGTGGCGTTGGt                                                                                                                                                                                                                >  2:175490/1‑251 (MQ=255)                                    gCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGAGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGGCGGGGCGTTGGTATAtt                                                                                                                                                                                                           >  2:247766/1‑251 (MQ=255)                                                          cGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAg                                                                                                                                                                                     <  1:324706/251‑1 (MQ=255)                                                            ggAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAAGCCAGTGACCTATG‑ACATTACCCGGATGCGTCGTGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGGCGCCGCTAGTGATAGTGACCAGTTTCGTGGTCGTTGCGGTGGTATATTCCCAGTGCGTCTGGCCGGTCAGGc                                                                                                                                                                                   <  2:28411/250‑1 (MQ=255)                                                                       acgggaaggcgaTAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACGGTAATCTCATTACCCTGCGGGCAAATCCAGTGACCTAGG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATAGTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAg                                                                                                                                                                        <  2:91117/245‑1 (MQ=255)                                                                                cgaTAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGc                                                                                                                                                               >  2:141509/1‑251 (MQ=255)                                                                                 gaTAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGAAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGCTGCGTGGGCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGGTAACTGAAGTAATCCGGc                                                                                                                                                               >  2:332528/1‑250 (MQ=255)                                                                                       cgcgCTGGCGGAATCATGGCTGGCAACGGGGACAACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTACCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTAGGGGCGTTGGTCTATTCCCAGATCATCTTGCCGGTCCGGCGATAACTGAAGTAATCCGGCCTcagc                                                                                                                                                         >  2:336559/1‑250 (MQ=255)                                                                                             ggCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTCCCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCg                                                                                                                                                   <  1:350421/250‑1 (MQ=255)                                                                                                        ggctggcAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATTCGTCGTGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCCCGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtgt                                                                                                                                       <  2:176515/251‑1 (MQ=255)                                                                                                         gctggcAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAAtgtgtg                                                                                                                                      >  2:369807/1‑251 (MQ=255)                                                                                                                 cGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGt                                                                                                                              <  2:228810/251‑1 (MQ=255)                                                                                                                  ggCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGtt                                                                                                                             >  2:236527/1‑251 (MQ=255)                                                                                                                    cgaccactggcatcaaaaaaCCCTGCGGGCCAATCCAGCGACCTATG‑ACCTTACCCGGATGCGTCGGGCGACCAAACAAGGCTTTGTTGGCCCAGCTCCACGCCAGTAGCGACTCGTACAAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTc                                                                                                                            >  2:155104/20‑250 (MQ=255)                                                                                                                          cTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGgttgt                                                                                                                     >  2:107394/1‑251 (MQ=255)                                                                                                                                        cccTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAgcgc                                                                                                       <  1:236527/251‑1 (MQ=255)                                                                                                                                                    aTCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGGTGGCCGCGCTCCACGCCAGTAGCGACGCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGGGCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATa                                                                                           <  2:301814/251‑1 (MQ=255)                                                                                                                                                         gTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCg                                                                                      >  1:324576/1‑251 (MQ=255)                                                                                                                                                                        tACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAg                                                                         <  1:373435/250‑1 (MQ=255)                                                                                                                                                                        tACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAg                                                                         <  2:358580/250‑1 (MQ=255)                                                                                                                                                                             ggATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTg                                                                   >  2:330097/1‑251 (MQ=255)                                                                                                                                                                                   gTCGGGCGACCCCACCAGGTTTTGTTGGCCCCGCTCCCCGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGcc                                                             >  2:211640/1‑251 (MQ=255)                                                                                                                                                                                     cGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGc                                                           <  1:141509/251‑1 (MQ=255)                                                                                                                                                                                     cGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGc                                                           >  1:351316/1‑251 (MQ=255)                                                                                                                                                                                       ggCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTa                                                         <  2:356192/251‑1 (MQ=255)                                                                                                                                                                                        gCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTAc                                                        <  1:247766/251‑1 (MQ=255)                                                                                                                                                                                            ccAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTg                                                    <  1:18938/251‑1 (MQ=255)                                                                                                                                                                                              aaaCCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGAt                                                  <  1:292301/251‑1 (MQ=255)                                                                                                                                                                                               aaCCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATa                                                 <  1:266488/251‑1 (MQ=255)                                                                                                                                                                                                          gttgGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGtt                                      >  2:107164/1‑251 (MQ=255)                                                                                                                                                                                                                cccGCTCCACGCCAGCAGCGACTCGTCCCAGTCACCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCCGGCGATACCTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCg                                 >  2:188353/1‑250 (MQ=255)                                                                                                                                                                                                                         cGCCAGTAGCGACTCGTCCCAGTCGCCGCTATTGATATTGCCCAGTTGCGTGGCCGCGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCCGGCGATACCTGAAGTACTCCGGCATCAGCAGAGCGCGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAgttgttgtt                       >  1:216945/1‑251 (MQ=255)                                                                                                                                                                                                                                aGCGACGCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCATCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTg                <  2:176206/251‑1 (MQ=255)                                                                                                                                                                                                                                   gACTCGTCCCAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTgcgc             >  1:268887/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccctgtcgccGCGATTGATATTGACCAGTTGCGGTGTCATGGCGTTTGTATAGTCCCAGGTCATCTTGCACGTCAAGCGATAACTGAAGGAATCCGGCATCAGCAGAGCGTGAGCGATGTGTTGAAGAAGTTCAGGTTGTGGCTCCGTCAGCGCACGCAGCTGATAAAGCGTAGTGAAGGTCAGGAGCTGGATGTCGCTACGGTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAg       <  2:15332/246‑1 (MQ=255)                                                                                                                                                                                                                                          cccAGTCGCCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAgg      >  1:70715/1‑251 (MQ=255)                                                                                                                                                                                                                                              gtcgccGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTGGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGAGGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGGTGTTGTGCCTGCGCCATTAGGCCAt  <  2:173625/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       GGCGTCTGGCTTTCGAAGCCCATCAATGACCAGGTGCCAGAAGAGAGATAAGCAGCACGTGAGCCGTTTAACGGCGAGGCGATAACCGCGCTGGCGGTATCATGGCTGGCAACGGCGACCACTGGAATCTCATTACCCTGCGGGCAAATCCAGTGACCTATG‑ACATTACCCGGATGCGTCGGGCGACCAAACCAGGCTTTGTTGGCCCCGCTCCACGCCAGTAGCGACTCGTCCCAGTCGTCGCTATTGATATTGACCAGTTGCGTGGTCGTGGCGTTGGTATATTCCCAGTTCATCTTGCCGGTCAGGCGATAACTGAAGTAATCCGGCATCAGCAGAGCGTGAGCAATGTGTGGAATAAGTTCAGGTTGTTGCTCCGTCAGCGCACGCAACTGATAAAGCGTATTGAAGGGCAGAAACTGGATGCCGCTACGTTGATAAATATCGCGTTTGCCGAGTTGTTGTTGTGCCTGCGCCATTAGGCCAT  >  NC_000913/4096640‑4097126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑