Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,730	A→G	E21G (GAG→GGG)	csqR →	DNA‑binding transcriptional dual regulator CsqR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,730	0	A	G	100.0%	91.8 / NA	30	E21G (GAG→GGG)	csqR	DNA‑binding transcriptional dual regulator CsqR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

GCTTTGGCGGTGGCGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATC  >  NC_000913/4074483‑4074974                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gcTTTGGCGGTGGCGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCg                                                                                                                                                                                                                                                   <  1:278393/251‑1 (MQ=255)         ggTGGCGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCCTGCTGATCGCCGGGCGTGGGtat                                                                                                                                                                                                                                            >  2:16075/1‑250 (MQ=255)               gcTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGTGTATATGAata                                                                                                                                                                                                                                     <  1:265234/251‑1 (MQ=255)                cTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAatat                                                                                                                                                                                                                                    >  1:114615/1‑251 (MQ=255)                  ggCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATAtt                                                                                                                                                                                                                                   <  2:11478/250‑1 (MQ=255)                     agcaaGGGGGGAGTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTGTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTGACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATtgatg                                                                                                                                                                                                                               <  1:347612/249‑1 (MQ=255)                                        gAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTggc                                                                                                                                                                                                            >  2:332491/1‑250 (MQ=255)                                            cagtcCGCTTCGCCAGCGGCGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGtc                                                                                                                                                                                                        >  2:141698/1‑251 (MQ=255)                                                    ttCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGc                                                                                                                                                                                                 >  1:74870/1‑250 (MQ=255)                                                                 gaGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTGTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCgg                                                                                                                                                                                   <  2:306108/250‑1 (MQ=255)                                                                  aGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCgg                                                                                                                                                                                   <  2:59817/250‑1 (MQ=255)                                                                                  acacGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAg                                                                                                                                                                  <  2:115333/251‑1 (MQ=255)                                                                                                                                     tttttGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTcc                                                                                                                 <  1:64987/249‑1 (MQ=255)                                                                                                                                              cttggagaaaaaaaTGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGGATGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTa                                                                                                       >  1:101714/11‑250 (MQ=255)                                                                                                                                                     tataAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                               >  2:68520/1‑251 (MQ=255)                                                                                                                                                            ggCCAGGGGGAGGGTTTTTCGAGGAATTTTCATGAGACTTACCGAACTAACCGGTAACCCTCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCTGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGGCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagc                                                                                        <  2:63076/250‑1 (MQ=255)                                                                                                                                                               cAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCGTCCAGCGTCGTTAATACGGCGTTCGagcagc                                                                                     <  1:297214/251‑1 (MQ=255)                                                                                                                                                                 gggTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCAGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                   <  2:254875/251‑1 (MQ=255)                                                                                                                                                                         gTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                           <  2:74870/251‑1 (MQ=255)                                                                                                                                                                            tttCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACGCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGCGCAGCGTGAGGTTTCGc                                                                         >  1:36881/1‑250 (MQ=255)                                                                                                                                                                                     aTTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAgg                                                                >  1:224619/1‑250 (MQ=255)                                                                                                                                                                                               gCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTACGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCg                                                     >  2:73831/1‑251 (MQ=255)                                                                                                                                                                                                   tACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAt                                                   <  1:352663/249‑1 (MQ=255)                                                                                                                                                                                                   tACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTg                                                 >  2:205053/1‑251 (MQ=255)                                                                                                                                                                                                   tACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGACGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAtt                                                  <  2:327119/250‑1 (MQ=255)                                                                                                                                                                                                                   aaCCCGCGGCACGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTTATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGtcacag                                  <  2:101714/250‑6 (MQ=255)                                                                                                                                                                                                                          ggCACGACCAACTCCTCATGCTGATCACCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATAtt                          <  1:160453/251‑1 (MQ=255)                                                                                                                                                                                                                             aCGACCAACTCCTCATGCTGATCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                       >  1:80690/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aTCGCCGGGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTAt   >  1:38671/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cGCCGGGCGTGGGTATATGACTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc  >  2:8157/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCTTTGGCGGTGGCGCTGGCAACAAGTGGGGATTTAGCGGAGTCAGTCCGCTTCGCCAGCGGTGTAGCGGCGTTAAAATGCACACGTCCCGGTGGACGAGCCGGGATCCCTGACTGTGATCAAACCCGATCTTTTTTGTCACTTTTTGTATAAAATGCCAGGGTGATGGTTTTTCGAGGAATTTTCATGAGCCTTACCGAACTAACCGGTAACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATC  >  NC_000913/4074483‑4074974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑