Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,906,029	A→G	M180T (ATG→ACG)	bglG ←	transcriptional antiterminator BglG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,906,029	0	A	G	100.0%	95.2 / NA	28	M180T (ATG→ACG)	bglG	transcriptional antiterminator BglG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

TTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGC‑ACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCAGTT  >  NC_000913/3905800‑3906274                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ttGATACTGTAAACCAATAAAAATGTCGATCCGT‑CCGCCACTTTGCCATGCTTGCGCGAAATTTTGCTTTACTGCTTGTTGTAATGATTCATCTCTCTCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAAATCAAGCTTTCTTCCTGGTAATTAGGGCTGAACTGAAATTTTCTTAATTACAGCATTTCGCGCGTTAACTGCGTGACACCTGCa                                                                                                                                                                                                                                   >  2:175470/1‑250 (MQ=255)           aaaCCAATAAAAATGGCGATCCGTTCCGC‑ACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTAAAGAATACGCCAGGATATAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCat                                                                                                                                                                                                                        >  2:260477/1‑251 (MQ=255)                           cGATCCGTTCCGC‑ACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTgg                                                                                                                                                                                                        >  1:295946/1‑251 (MQ=255)                                                cATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGc                                                                                                                                                                                    <  2:259815/251‑1 (MQ=255)                                                         gggTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTGTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGcc                                                                                                                                                                           <  1:207348/251‑1 (MQ=255)                                                              aTTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGc                                                                                                                                                                                                                <  2:212701/209‑1 (MQ=255)                                                              aTTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGc                                                                                                                                                                                                                >  1:212701/1‑209 (MQ=255)                                                                     tttACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCtt                                                                                                                                                                <  1:329161/250‑1 (MQ=255)                                                                               gttgtAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGc                                                                                                                                                     >  1:98421/1‑251 (MQ=255)                                                                                                             aaGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAAc                                                                                                                       >  2:163957/1‑251 (MQ=255)                                                                                                                cATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGct                                                                                                                    >  1:273035/1‑251 (MQ=255)                                                                                                                        aGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTTATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATGTGGGCACGGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGTTAACTGCTCGCCCAACCGTTTATCAATAATGGGTAACTCGTCTTcccc                                                                                                            <  2:227796/251‑1 (MQ=255)                                                                                                                                    gATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTACTGGAAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGCActc                                                                                                >  1:60349/1‑251 (MQ=255)                                                                                                                                                 gATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGc                                                                                   <  1:11772/251‑1 (MQ=255)                                                                                                                                                         aCCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGat                                                                           <  1:127879/251‑1 (MQ=255)                                                                                                                                                         aCCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGat                                                                           >  1:116722/1‑251 (MQ=255)                                                                                                                                                                   gATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGc                                                                 <  1:102442/251‑1 (MQ=255)                                                                                                                                                                   gATAACTCAAGCTTTCTTCCTGGTAATTAAGGCGGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACGTCATCTTTCGGTAACTGCACGCCCAACCTTTTATCAATAATGGTTAACTCTTCTTCCCCTAGCTGGAACGCCTTCGGGTAAAGCCTCTGGATATCCCACAg                                                                  <  2:177453/250‑1 (MQ=255)                                                                                                                                                                     tAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCt                                                                                                                                                                 <  2:373924/153‑1 (MQ=255)                                                                                                                                                                     tAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCt                                                                                                                                                                 >  1:373924/1‑153 (MQ=255)                                                                                                                                                                           aaGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTa                                                                                                          >  2:107227/1‑202 (MQ=255)                                                                                                                                                                           aaGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTa                                                                                                          <  1:107227/202‑1 (MQ=255)                                                                                                                                                                                         gTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGt                                           <  2:273035/251‑1 (MQ=255)                                                                                                                                                                                           aaTTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGtt                                          <  1:57060/250‑1 (MQ=255)                                                                                                                                                                                                            aaTTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTg                                                                                                      >  2:259326/1‑173 (MQ=255)                                                                                                                                                                                                            aaTTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTg                                                                                                      <  1:259326/173‑1 (MQ=255)                                                                                                                                                                                                             aTTTTATTAATTGCAGCATTTCGCGCGTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATc                       <  1:105165/251‑1 (MQ=255)                                                                                                                                                                                                                          cagtaTTTCGCGCGTTACCTGCGTGACACCTGCAACAGCCTCCATATTTCCGCCCAGTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGAAAt          <  2:287296/247‑1 (MQ=255)                                                                                                                                                                                                                                  cgcgcgTTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCAGtt  >  2:148208/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TTGATACTGCAAACCAATAAAAATGGCGATCCGTTCCGC‑ACATTGCCATGCTTGCGGGTAATTTTGTTTTACTGCTTGTTGTAATGATTCATCACTATCGTTAATTGAAGCATGTTCAAGAATACGCCAGGATAAAAACTTCAGATGTGTAACCAGTCGCTGATAACTCAAGCTTTCTTCCTGGTAATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCAGTT  >  NC_000913/3905800‑3906274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑