Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,637	C→A	L87L (CTC→CTA)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,637	0	C	A	100.0%	105.5 / NA	32	L87L (CTC→CTA)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/16);  total (16/16)

CGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCT  >  NC_000913/3869409‑3869870                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          cgggccGGCAGGAGCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACGGAAGGTTTCAGCAAACCCTGCGGCGGGCTGCTGGCACCGGACGCGCAGCGGTCTTTTATTCGGGATGGGCTGACGCTTCCTGTCGATGTGATCGCGAATCTGCAGATTTTCAGCGTCAAAACCGTCGGCGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCt                                                                                                                                                                                                                     <  2:342832/247‑1 (MQ=255)                  cGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGc                                                                                                                                                                                                    >  2:33142/1‑251 (MQ=255)                  cGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGc                                                                                                                                                                                                    >  1:126036/1‑251 (MQ=255)                  cGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGc                                                                                                                                                                                                    >  1:327383/1‑251 (MQ=255)                                 tAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtg                                                                                                                                                                                     >  2:217470/1‑251 (MQ=255)                                  aGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTtgt                                                                                                                                                                                    <  2:227715/251‑1 (MQ=255)                                      ggCAAAATGCAGGTGATCGCGCTGGATAACAAACACCAGTGCGGTACTGAAGGTTTCACCAAAACCTGCGGCGGTCTGCTGGCACCGGCCGCGCAGCGATCTTTTATTCGCGATGGGCCGACGCTTCCTGTCGCTGTGATCGCCAATCCGCAGATTTTCCGCGTCAACACCCTCGACGTCGCCGCATCGCTAACCCCCAACTACCAGCGAAGCTAAATCAATATTTATCGCCATGCTTTCGACTTGTGGAt                                                                                                                                                                                >  2:323405/1‑251 (MQ=255)                                      ggCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGAt                                                                                                                                                                                >  1:121433/1‑251 (MQ=255)                                                  gTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGAt                                                                                                                                                                    >  1:112321/1‑251 (MQ=255)                                                    gATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGAACGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATc                                                                                                                                                                   <  2:260813/250‑1 (MQ=255)                                                                tAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTg                                                                                                                                                      >  2:81953/1‑251 (MQ=255)                                                                                                  aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTg                                                                                                                    >  1:272139/1‑251 (MQ=255)                                                                                                  aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTg                                                                                                                    <  2:112321/251‑1 (MQ=255)                                                                                                  aaaCCCTGCGGCGGTCTGCGGGCCCCGGACGCGCAGCGATCTTTTATTCGGGATGGGCTGACGCTTCCTGTGGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCAACGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTAATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTg                                                                                                                    <  2:260807/251‑1 (MQ=255)                                                                                                                   gctgCCACCGGACGCGCTGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTACTCCGCAGATTTTCAGCGTCAAACCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGTTATATCAATATTAATCGCCATGCTTTCGACTTGGGGGTGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATaa                                                                                                     <  2:182731/249‑1 (MQ=255)                                                                                                                         aCCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCCGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATg                                                                                             >  1:22289/1‑251 (MQ=255)                                                                                                                                  gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGTTGTGATCGCTAATCCGCAGATTTTCTGCGTCAAAACCGTCGACTTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATAAATATTAATCGCCATGTTTTCTACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGTCATGTCATTTTTc                                                                                    <  2:52096/251‑1 (MQ=255)                                                                                                                                   aaGCGATCTTTTATGCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCg                                                                                   <  2:272139/250‑1 (MQ=255)                                                                                                                                         tCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCg                                                                                                                                                                                          >  1:120740/1‑142 (MQ=255)                                                                                                                                         tCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCg                                                                                                                                                                                          <  2:120740/142‑1 (MQ=255)                                                                                                                                             ttATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACgg                                                                          <  1:33142/250‑1 (MQ=255)                                                                                                                                                     gATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCAGCAGATTTTCAGCGCCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGc                                                                  >  2:323506/1‑250 (MQ=255)                                                                                                                                                                             gggATCGCTAATCCGCAGATTTTCATCGTAAAAACCGCCGGCGTCGCAGACTCACTAGCGCGTAACTACACGCGAAGCTATAGCATAATTAATCTCCATGCTTTCGACTGGTGGATAAAATCGCTGATCCCCGCCAGCGTTGAGGTTGATCACGACAGCCTGTGCCGAAAAATCTTGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGTCGGCTGGGAGCATCATATTACCGCCCGCTATCTGGt                                         <  2:375390/249‑1 (MQ=255)                                                                                                                                                                               gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCg                                       <  1:146423/251‑1 (MQ=255)                                                                                                                                                                                  cGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATAGTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTg                                    <  1:53588/251‑1 (MQ=255)                                                                                                                                                                                    cTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTgg                                                                                                                   <  2:172164/170‑1 (MQ=255)                                                                                                                                                                                    cTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTgg                                                                                                                   >  1:172164/1‑170 (MQ=255)                                                                                                                                                                                        tCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATg                              >  2:109346/1‑251 (MQ=255)                                                                                                                                                                                                  ttCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACt                     <  1:82480/250‑1 (MQ=255)                                                                                                                                                                                                        gTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGt              >  1:338451/1‑251 (MQ=255)                                                                                                                                                                                                                cGTCGAAGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGTTGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTCCCGCCCGCTATCTGGTCGGTGCCGATGGCGCCAACTCGATGGTGCGGCGAc      >  2:334690/1‑251 (MQ=255)                                                                                                                                                                                                                    gACGTCGCCGCACCGCTAACGCTTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCACCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtct  <  2:269926/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          CGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCT  >  NC_000913/3869409‑3869870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑