Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,162	C→T	H255Y (CAC→TAC)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,162	0	C	T	100.0%	128.4 / NA	38	H255Y (CAC→TAC)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

GGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGG  >  NC_000913/3822919‑3823399                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              ggTATCCACCACATTAAAACCGAACTCGAATAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAAGACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATGTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGAt                                                                                                                                                                                                                                        <  1:166047/251‑1 (MQ=255)       ccccacATTAAAACCGAACGCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGa                                                                                                                                                                                                                                  <  2:246248/249‑1 (MQ=255)       caccacATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGAGTTTTTACTCGATCATGGa                                                                                                                                                                                                                                  <  2:246255/251‑1 (MQ=255)                            gAAGAGCTGGGTTTTGAGGCGCTGTAGCCCAACCGTTAGCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCGTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGGCGCGAGAAGCAGCTTCATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTGTGTACTCGATCATGGACATCTACGCTTTCCGCGTGAt                                                                                                                                                                                                             <  2:188185/251‑1 (MQ=255)                             aaGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATc                                                                                                                                                                                                            >  2:193123/1‑251 (MQ=255)                                 gCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCa                                                                                                                                                                                                        <  2:343237/251‑1 (MQ=255)                                     ggTTTTGAGGCGCTGGATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAAGCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCAGCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGTGTTTTTACTCGATCATGGACATCTACGCGTTCCGCGTTATCGTCAATGa                                                                                                                                                                                                    <  2:212318/251‑1 (MQ=255)                                      gTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTCATCCAAGAAGTCGCGAAAGCCGCGCGCGCCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAACGGCGCTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTAGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGCGCAGCGTTTTTACTCGATAATGCACATCTACGCTTTCCGCGTGATCGTCAATGAt                                                                                                                                                                                                   >  1:230867/1‑251 (MQ=255)                                                 cTGTCTCCCAACCGTTATCGCGTAATCCAAGAAGTGGTGTAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGAGTTTGCAGTAAGCGGGAATACCGTGCCGCGCCAGTGGGCGCGAGAAGCATCTTTATTCGATTTACTCCAAAGTGGGGCTCAAAGAGCAGCGTTTTTACTCGCTCATGTACATCTAGGCTTTCCGCGTGCTCGTCCATAATTTTGAAACCTg                                                                                                                                                                                        >  2:197732/1‑251 (MQ=255)                                                       cccAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATc                                                                                                                                                                                   <  1:193123/250‑1 (MQ=255)                                                         cAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                <  1:109487/251‑1 (MQ=255)                                                                        aaTCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCATAAGATGCTGTCTGAAATCGAAGGGCGTGTGCAGGAAGCGGGAATACCGTGCCTCGTCAGTGGTCGCGAGAGGCATCTTTATTGGATTTACTGCAAAGTGGTGCTCAAAGAGCGGCGTTTTTACTCGATCATGGACATCTACGCTGTCCGCATGATCGTGAATGATTCTGACATGTGTTATCTCGTGCTGGGCCAGATg                                                                                                                                                                  <  2:216937/250‑1 (MQ=255)                                                                              agaagTGGTGAACGCCGCGAGCGGCAACCGTAAAGAGCTGCACCAGAAGATTATTTCTGAAATCGAAGGGCGTTTGCAGGACGAAGGACTACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGcc                                                                                                                                                           >  2:213829/1‑251 (MQ=255)                                                                               gaagTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCt                                                                                                                                                          <  1:201673/251‑1 (MQ=255)                                                                                 agTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATTCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGACGCATCTTTATTCGATTTACTGCGAAATGGCGATCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGGTTCTGACACCTGTTATCGCGTGCTGGGACGGATGCACAGCCTGt                                                                                                                                                        >  1:31732/1‑251 (MQ=255)                                                                                   tggtgAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTAc                                                                                                                                                      >  1:103471/1‑251 (MQ=255)                                                                                            ccGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGt                                                                                                                                             >  1:73930/1‑251 (MQ=255)                                                                                             cgcgcgcgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTc                                                                                                                                            <  1:20243/251‑1 (MQ=255)                                                                                               cgcgcgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCACAGCCTGTACAAGCCGCGTCCg                                                                                                                                          <  2:99606/251‑1 (MQ=255)                                                                                                   cgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCcgc                                                                                                                                               >  1:44948/1‑242 (MQ=255)                                                                                                   cgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCAGGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCcgc                                                                                                                                               <  2:44948/242‑1 (MQ=255)                                                                                                           gTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGCAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaa                                                                                                                               >  1:126221/1‑250 (MQ=255)                                                                                                           gTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa                                                                                                                              <  2:38743/251‑1 (MQ=255)                                                                                                           gTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaa                                                                                                                               >  2:179279/1‑250 (MQ=255)                                                                                                                   atgatCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTatat                                                                                                                      >  1:129038/1‑251 (MQ=255)                                                                                                                                 tctttctGAAATCGAAGGGCGTTTGCAGGAAGCGGGACTACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTCTTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGTTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTCCAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa                                                                                                         <  2:189603/250‑1 (MQ=255)                                                                                                                                                                   ggAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat                                                                      <  2:59248/251‑1 (MQ=255)                                                                                                                                                                    gAATACCGTGCCCCGTCAGTGGTCGCGAGCAGCATCTTTACTCGATTTACTGCAAAATGGTGCTCCAAGCGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGATCGGCTATCAGTCTTTGCACACCTCGATGATc                                                                     >  2:256569/1‑251 (MQ=255)                                                                                                                                                                                       tGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                                  >  1:278306/1‑251 (MQ=255)                                                                                                                                                                                         gTCGCGAGAAGAATCTTTATTCGATTTACTGCAAAATGGTCCTCAAAGAGCAGCGTCTTTACTCGCTCATGGACATCTACGCTTTCCGAGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCAAGATGCACAGCCTGTACAAGCCGCGTCCGGGACGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGtt                                                >  1:366390/1‑251 (MQ=255)                                                                                                                                                                                             cgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACACTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAgg                                            >  2:182479/1‑251 (MQ=255)                                                                                                                                                                                                gaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc                                         >  2:107775/1‑251 (MQ=255)                                                                                                                                                                                                gaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGAAGCGTTTTTACTCGATCATGGACATATACGCTTTCAGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTACAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGAGTGCCGGTTGAGGTcc                                         >  1:334959/1‑251 (MQ=255)                                                                                                                                                                                                                     cTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATgg                    >  1:337418/1‑251 (MQ=255)                                                                                                                                                                                                                             tGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCAAGATCCGTACCGAAGATATGGACCAGATg            >  1:358814/1‑251 (MQ=255)                                                                                                                                                                                                                              ggTGCTCACAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCCTTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATgg           >  2:64426/1‑251 (MQ=255)                                                                                                                                                                                                                                       agagCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATgg  <  2:292714/251‑1 (MQ=255)                                                                                                                                                                                                                                       agagCAGAGTTTTTGCTCGATCTAGGAAATCTACGCTTTCAGCGAGATCGTCAATGATTCTGACACCTGTTATCGCGTGCAGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATAACGCCATTCCAAAAGCGAACGGCTATAAGCCTTTGCACACATCGATGATCGGCCCGCACGGTGTGCAGGGTGAGGTACAGATCCGTACCGAAGATATGGATCAGAtagagtcgcagg  >  2:230537/1‑240 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              GGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGG  >  NC_000913/3822919‑3823399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑