Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,770,892	T→C	D163G (GAC→GGC)	yibH ←	inner membrane protein YibH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,770,892	0	T	C	100.0%	131.2 / NA	42	D163G (GAC→GGC)	yibH	inner membrane protein YibH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

GGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTGAATAAGCTGAT  >  NC_000913/3770651‑3771135                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ggCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCg                                                                                                                                                                                                                                            >  1:275751/1‑251 (MQ=255)      cTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAAc                                                                                                                                                                                                                                       >  2:301180/1‑251 (MQ=255)        gCGTATGTCCCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACg                                                                                                                                                                                                                                      <  2:110125/250‑1 (MQ=255)            aTGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCTCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGAtt                                                                                                                                                                                                                                  <  2:135822/250‑1 (MQ=255)                             aGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGcc                                                                                                                                                                                                                <  1:84943/251‑1 (MQ=255)                                                   tGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAtt                                                                                                                                                                                           <  1:235637/250‑1 (MQ=255)                                                              cgAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCGTTCACGTACCGTTGATAATTTTTAAACAGACg                                                                                                                                                                               >  2:70384/1‑251 (MQ=255)                                                               gAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                                                                                                                                                                              >  1:228014/1‑251 (MQ=255)                                                                aaTGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                                                                                                                                                                             <  1:261141/251‑1 (MQ=255)                                                                aaTGACAGTCTGCGCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                                                                                                                                                                             <  1:274727/251‑1 (MQ=255)                                                                       gTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACAATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTc                                                                                                                                                                      >  2:329719/1‑251 (MQ=255)                                                                         cTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCg                                                                                                                                                                    >  1:190205/1‑251 (MQ=255)                                                                                             cTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTg                                                                                                                                                >  1:14986/1‑251 (MQ=255)                                                                                                 aGTAAGATGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATCCTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTACGCTGAAACATGGGTGGTGTTGGCCt                                                                                                                                            >  2:329152/1‑251 (MQ=255)                                                                                                                tAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCt                                                                                                                             >  1:160111/1‑251 (MQ=255)                                                                                                                tAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCGCTGGTTCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGc                                                                                                                              <  2:277968/250‑1 (MQ=255)                                                                                                                 aaGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCGCCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGAGAATTTGTAAACAGACGGTGGCGCTCCGCTTAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCt                                                                                                                             <  1:230441/250‑1 (MQ=255)                                                                                                                 aaGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTACGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTg                                                                                                                            >  1:248705/1‑251 (MQ=255)                                                                                                                                   tGCTCGCCGTTACCCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCCCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGACATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCCAGTAACGTTGATAATTTTTAAACAGCCGGTCGCGCTCCTCTGAAACCTGGGTGGTGTTGTCCTGCGCTTCAGTGAGCTGCGCACGCACCGTCTTtat                                                                                                          >  2:215463/1‑251 (MQ=255)                                                                                                                                              aaCCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTAGAGTATGCGTc                                                                                                <  1:325584/250‑1 (MQ=255)                                                                                                                                                  aTACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAg                                                                                                                               >  2:338601/1‑215 (MQ=255)                                                                                                                                                  aTACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAg                                                                                                                               <  1:338601/215‑1 (MQ=255)                                                                                                                                                    aCTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGtca                                                                                         <  1:361533/251‑1 (MQ=255)                                                                                                                                                                   ctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGAAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTg                                                                            <  2:253002/249‑1 (MQ=255)                                                                                                                                                                    tGGATCTGCGCCTGCTCCGCCACAGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGa                                                                           >  2:242032/1‑249 (MQ=255)                                                                                                                                                                             gcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAAc                                                                >  2:48557/1‑251 (MQ=255)                                                                                                                                                                                        cACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCAGCGCATAGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTACACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCt                                                                                                                             >  1:342769/1‑179 (MQ=255)                                                                                                                                                                                        cACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATGTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCGGCCGTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGTTGGTGTTGGCCTGCGCTTCAGTGAGCt                                                                                                                             <  2:342769/179‑1 (MQ=255)                                                                                                                                                                                              gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGa                                                >  1:369473/1‑250 (MQ=255)                                                                                                                                                                                                cTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCACCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAAcc                                             >  1:160395/1‑251 (MQ=255)                                                                                                                                                                                                  ttCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACGCGCGCCTGGTAACGAAcc                                             <  2:151827/249‑1 (MQ=255)                                                                                                                                                                                                   tCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGAATCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGCCGTCTGTCAACTCGCGCCTGGTACCGAACCggg                                          >  1:208614/1‑251 (MQ=255)                                                                                                                                                                                                     aCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTc                                        <  1:48557/251‑1 (MQ=255)                                                                                                                                                                                                                      gAGGAAATTGTGCCGCGCAGCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGGCTTTCAAGTAACGTTGATAATTTGTAAACAGGCGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCt                       <  2:160395/251‑1 (MQ=255)                                                                                                                                                                                                                            aTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCGCGCCCt                 <  2:275751/251‑1 (MQ=255)                                                                                                                                                                                                                              tttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCtt                <  1:259125/250‑1 (MQ=255)                                                                                                                                                                                                                               ttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCtttt              >  1:320979/1‑251 (MQ=255)                                                                                                                                                                                                                               ttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCGCGCCCttt               <  1:301180/250‑1 (MQ=255)                                                                                                                                                                                                                                 gCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTg             <  2:30080/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTGAATAAGCt     >  2:228651/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAACCAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTGAATAAGCTGat  >  2:332478/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTGAATAAGCTGat  >  2:56940/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    GGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATTGCTCGCCGTTAACCATACTGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGCTGCGCACGCAGCGTCTTTATATTATGCGTCGCCGTCATCAGGTCAGCCTGAAGTCTGTCAACTCGCGCCTGGTAACGAACCGGGTCGAGCTTAAAAAGCACCTCGCCCTTTTGAATAAGCTGAT  >  NC_000913/3770651‑3771135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑