Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,102	T→C	L90L (TTA→TTG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,102	0	T	C	100.0%	206.5 / NA	60	L90L (TTA→TTG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/37);  total (23/37)

GTCAGACATATCGCTGGCAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCC  >  NC_000913/3728862‑3729341                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                gTCAGACATATCGCTGGCAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGtt                                                                                                                                                                                                                                       <  1:363011/251‑1 (MQ=255) gTCAGACAAATCGCTGGCAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGtg                                                                                                                                                                                                                                       <  2:3716/251‑2 (MQ=255)      aCATATCGCTGGCAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGa                                                                                                                                                                                                                                  <  1:65503/251‑1 (MQ=255)                  cAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCa                                                                                                                                                                                                                        >  2:359421/1‑249 (MQ=255)                    aaCTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCa                                                                                                                                                                                                                        <  1:324869/247‑1 (MQ=255)                    aaCTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCa                                                                                                                                                                                                                        >  2:324869/1‑247 (MQ=255)                                aTACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCAt                                                                                                                                                                                                        <  2:7370/251‑1 (MQ=255)                                aTACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTGCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCAt                                                                                                                                                                                                        <  1:22421/251‑1 (MQ=255)                                     cAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGc                                                                                                                                                                                                   <  2:117604/251‑1 (MQ=255)                                      aGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGcc                                                                                                                                                                                                  <  2:82475/251‑1 (MQ=255)                                              gTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATAc                                                                                                                                                                                          <  2:108398/251‑1 (MQ=255)                                                     ttCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCaa                                                                                                                                                                                      <  1:312125/248‑1 (MQ=255)                                                     ttCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAAt                                                                                                                                                                                     <  2:265457/249‑1 (MQ=255)                                                      tCGGTAATAATACTTTGTCGATTTGACGGAATATCCCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGc                                                                                                                                                                                   <  2:19563/250‑1 (MQ=255)                                                        ggTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCttt                                                                                                                                                                                <  1:24702/251‑1 (MQ=255)                                                         gTAATAATACTTTGTAGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCCCAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCttt                                                                                                                                                                                >  2:211189/1‑250 (MQ=255)                                                                             tGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTg                                                                                                                                                           >  1:317670/1‑251 (MQ=255)                                                                                  gAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGc                                                                                                                                                      >  1:158429/1‑251 (MQ=255)                                                                                  gAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGc                                                                                                                                                      >  2:303803/1‑251 (MQ=255)                                                                                         tcCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGc                                                                                                                                               >  2:27569/1‑251 (MQ=255)                                                                                           cGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGTAATTTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGTCCAGAGCtt                                                                                                                                             <  2:182593/251‑1 (MQ=255)                                                                                            ggCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCttt                                                                                                                                            <  1:27569/251‑1 (MQ=255)                                                                                              cTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTc                                                                                                                                           >  1:277273/1‑250 (MQ=255)                                                                                                cATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGAGCGCCCAGAGCTTTCa                                                                                                                                          <  1:108503/249‑1 (MQ=255)                                                                                                    ccGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTgcgc                                                                                                                                    >  2:105855/1‑251 (MQ=255)                                                                                                         gAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCTAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATc                                                                                                                                <  2:205772/250‑1 (MQ=255)                                                                                                         gAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGAt                                                                                                                                 <  2:117028/249‑1 (MQ=255)                                                                                                           aCCCATAGCAAGTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGACCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGt                                                                                                                             <  2:223843/251‑1 (MQ=255)                                                                                                                 aGCAATGTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACGCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCGTTAACGTCCTGCAGATAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtgcctg                                                                                                                       <  1:216010/251‑1 (MQ=255)                                                                                                                  gCAATTTAGGCGCAGTAACTCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtgcctgc                                                                                                                      >  1:120069/1‑251 (MQ=255)                                                                                                                    aaTTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTg                                                                                                                                                                                                                  >  2:40319/1‑157 (MQ=255)                                                                                                                    aaTTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTg                                                                                                                                                                                                                  <  1:40319/157‑1 (MQ=255)                                                                                                                    aaTTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGcca                                                                                                                    <  2:374396/251‑1 (MQ=255)                                                                                                                         aGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCACCACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACt                                                                                                               <  1:317872/251‑1 (MQ=255)                                                                                                                               aGTAACTCCGGGCATCATCAGGTTGCCGGAAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCACCACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAGCGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCg                                                                                                         >  2:364303/1‑251 (MQ=255)                                                                                                                                  aaaTCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCggg                                                                                                       <  2:352612/250‑1 (MQ=255)                                                                                                                                   aaTCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTc                                                                                                     >  1:264833/1‑251 (MQ=255)                                                                                                                                               atcaGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAcc                                                                                          >  1:265599/1‑250 (MQ=255)                                                                                                                                                           gTAATCACACGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCgg                                                                             >  2:17979/1‑251 (MQ=255)                                                                                                                                                                cACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGcgcg                                                                        >  1:146725/1‑251 (MQ=255)                                                                                                                                                                  cccGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTGGTTCCGACCAGAGTGGATGGGGGCGCGaa                                                                      <  2:335708/251‑1 (MQ=255)                                                                                                                                                                    cgcgATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAAc                                                                    <  1:359421/251‑1 (MQ=255)                                                                                                                                                                             ggAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCtt                                                           <  2:135700/251‑1 (MQ=255)                                                                                                                                                                             ggAACTCGCCCTTCCAGCACAGTGCACTCTTGCGAACAGCGCCCGTCGTTCCACAAACTGGAATGGCGCAACACCCGTTGCTGAGCATCCAGCCAGGTTGCTCCGTGCATCTGGCCGGCCATACCCAATGCTTTAACGTACTGCAGAGATTGCTGATAGTCCAGCGCTTTCATTGCGAGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCtt                                                           >  2:343214/1‑251 (MQ=255)                                                                                                                                                                                       cTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTgc                                                 >  1:86479/1‑251 (MQ=255)                                                                                                                                                                                          ccAGCAAAGTGCACTCTTGCGCACAGCGCCAGTAGTTCCACAAAATGGCAGGGCGCACCACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGCGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTgcgc                                               >  1:338397/1‑250 (MQ=255)                                                                                                                                                                                              cAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCGGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGCTTGCgcagca                                           >  2:325109/1‑250 (MQ=255)                                                                                                                                                                                              cAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa                                          <  2:136995/251‑1 (MQ=255)                                                                                                                                                                                                  gTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGGTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCTCCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAacccc                                      <  1:41191/251‑3 (MQ=255)                                                                                                                                                                                                     cacgctTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTc                                   <  2:145366/247‑1 (MQ=255)                                                                                                                                                                                                         cctGCGCACAGCGCCCGTCGTGCCACAAAATGGCAGGGCGCAACGCCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGGTCCGGGTCTTGGTCCGACCAGAGTGGAGGCGGGCGCGAAACGGTGAGCGTTTCCGTATGCGCACCAAGCAGCTCAccc                               <  2:54532/249‑1 (MQ=255)                                                                                                                                                                                                              gcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTc                          >  2:230232/1‑251 (MQ=255)                                                                                                                                                                                                              gcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTc                          <  1:179807/251‑1 (MQ=255)                                                                                                                                                                                                                 caGCGCCCGGCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCATTTGCCTGCCGCCACGGTGCCGTGTCTTGTTCCGACCAGAGTGGATGCGGGGGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGtt                       <  1:371300/251‑1 (MQ=255)                                                                                                                                                                                                                   gcgcCCGTCGTTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGa                     <  2:14193/251‑1 (MQ=255)                                                                                                                                                                                                                        cgtcgtTCCACAAAATGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaaa                <  1:123812/251‑1 (MQ=255)                                                                                                                                                                                                                            gtTCCCCAAAATGGCAGGGCGCAACACCAGCTGCTGCGCATCCAGCAAGGTTGCTCAGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATa             >  2:358293/1‑250 (MQ=255)                                                                                                                                                                                                                              ccctcaAAATGGCAGGACGCAACACCCGTTGCTGAGCATCCAGCAAGGCTGCTCCGTGCATCTTGCCGTCAATACCCATTTGTTTAACGTCCAGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTGCCGTTTGCGCAGCAACCACCGCACCCTGCTCGTTGAGCAAAATAAca          <  2:192602/247‑2 (MQ=255)                                                                                                                                                                                                                               ccACAAAATGGCAGGGCTCAACACCCGTTGCTGAGCATCAAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATTCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACt          <  2:192614/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTGGCAGGGCGCAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGcc  >  2:297904/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                GTCAGACATATCGCTGGCAAACTCCCCCGTCATACGCAGACGCAAGTAATCTTTCGGTAATAATACTTTGTCGATTTGACGGAATATCTCCGGCTCATGCCGCTGAACCCATAGCAATTTAGGCGCAGTAAATCCGGGCATCATCAGGTTGCCGGTAATCACCCGCGATTGCGGAACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCC  >  NC_000913/3728862‑3729341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑