Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	118.0 / NA	34	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

CTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGAT  >  NC_000913/3682268‑3682757                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cTACGCAGTTCCCGCCCAGGTTCGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTAtt                                                                                                                                                                                                                                                 >  2:242350/1‑251 (MQ=255)  tACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTg                                                                                                                                                                                                                                                >  1:27466/1‑251 (MQ=255)             cgacaagtttAGTCAGCGCCCGAGCTTCTGACATAAAGCGGTCGATCCCGAGGACCAGCGCCAGACCCGCTACCGGCAAAGGGCCCCCCGAAGAGAGCGTCGCCCCCAGCACGATAAAGCCCCTACCCTTTACCCCTGCCGCCCCTTAAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTTTTAGTGGCCTGGGCGATAAACTCCGCCGCTATTGTCAGGTATATc                                                                                                                                                                                                                                     <  1:367204/243‑1 (MQ=255)                                                                                            acgccccacctcaaggagCGCCGCCGCCACAACAATAAAGCCCCTACCCTTTCCCCCTGCCGCCCCTTAAGAAAAAAGCAGCAACACGATAAACGGCGTTATTGGGGGGACGATATCCATCTGACTGTTAGTGGCCGGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCa                                                                                                                                                      <  2:37545/236‑1 (MQ=255)                                                                                              ggCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGAGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCACCATc                                                                                                                                                    >  2:120615/1‑251 (MQ=255)                                                                                                   aCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTc                                                                                                                                               >  2:172997/1‑251 (MQ=255)                                                                                                         gagagCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCAt                                                                                                                                         >  2:299811/1‑251 (MQ=255)                                                                                                                       caccACGAAAAAGCCACTCCCCTTTCCCCCGCCCGCCCCTTTAGAAGAAAGCAGAAACAGGATTAACATCGGAATTGGGTGGACGATACCCTTCTGACTGTTAGTGGCCGGGGCTATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCg                                                                                                                           <  1:142931/248‑1 (MQ=255)                                                                                                                           ccaataaccccccccccctttcccccCGCCGCCCCTTTAGAAAAAATCAGCAACACTATTACCGGCGTGTTTTGGTGGCCTATACCCTTCTTACTTTTAGTGCCCTGGGCGATAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCATCCCCACCACCGATTTACTGCAGCCGATTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTc                                                                                                                       <  1:142724/229‑1 (MQ=255)                                                                                                                           aCGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTc                                                                                                                       >  1:10092/1‑251 (MQ=255)                                                                                                                               aaaaccccccccccgtccccccCGCCGCCCCTTAAAAAGAAAGCAAAAACACGATTAACAGTGTGATTTGGTGGACGATTCCCATCTGACTGTTAGGGGCCGGGGAAAAAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGaa                                                                                                                   <  2:26340/233‑1 (MQ=255)                                                                                                                                  ccccaccccccGTCACCCCTGCCGCCCTTTTAAAAAAACGCAGCAACCCGATAAACAGCGGGTTTGGGTGGAATATATCCATCTGACTGTTAGTGGCCGGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGAt                                                                                                                <  2:282109/243‑1 (MQ=255)                                                                                                                                  aGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGa                                                                                                                    >  1:157940/1‑247 (MQ=255)                                                                                                                                        tACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTc                                                                                                          >  1:41537/1‑251 (MQ=255)                                                                                                                                        tACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTc                                                                                                          >  1:43814/1‑251 (MQ=255)                                                                                                                                             gTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTcccc                                                                                                       >  2:155901/1‑249 (MQ=255)                                                                                                                                               tcccccGGCCGCCCCTTTAAAAGAAAGCAGCAACAGGATTAACAGCGTGATTGGGTGGACGATACCCAGCTGACTGTTAGGGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTGCTCCATCTTGTCGAGCATACGGGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGt                                                                                                    <  1:334591/248‑1 (MQ=255)                                                                                                                                                              ttaaaagaaagacacaaaaacGATAAACGGCGTGATGGGGTGAACGATACCCATCGGATTGTTGGGGCCCTGGGCAAAAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttct                                                                                     <  2:353498/231‑1 (MQ=255)                                                                                                                                                                tAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                                   >  2:28079/1‑250 (MQ=255)                                                                                                                                                                 agaagaAAGCAGCAACCCGATTAACAGGGTGATTTGGGGGACGATACCCATCTGACTGTTAGTGGCCGGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAAt                                                                                               <  1:281037/237‑1 (MQ=255)                                                                                                                                                                 agaagaAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAAt                                                                                               >  2:281037/1‑237 (MQ=255)                                                                                                                                                                 agaagaAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCAc                                                                                 >  1:140991/1‑251 (MQ=255)                                                                                                                                                                 agaagaAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCAc                                                                                 <  1:302438/251‑1 (MQ=255)                                                                                                                                                                  gaagaaAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACg                                                                                >  1:256335/1‑251 (MQ=255)                                                                                                                                                                              aaCACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGAt                                                                    >  2:28243/1‑251 (MQ=255)                                                                                                                                                                                        aCAGCGTGATTTGGTGGACGATAGCCATGTGACTTTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAAGCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAg                                                          <  1:242350/251‑1 (MQ=255)                                                                                                                                                                                          ggCGTGTTTTGGTGGACGATATCCTTCTGACTTTTAGTGGCCTGGGCAATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGAt                                                        <  2:157940/250‑1 (MQ=255)                                                                                                                                                                                           gCGTGATTTGGTGGACGATATCCATCTGCCTGTTAGTGGCCTGGGCGATAAACACCGCCGCTCTTGTCAGGTATCTCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGCGTTTCTCCATCTTGTCGAGCACACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGAt                                                        >  2:227092/1‑250 (MQ=255)                                                                                                                                                                                                aTTTGGTGGACGATACCCATCTGCCTTTGAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGaa                                                  <  1:288967/251‑1 (MQ=255)                                                                                                                                                                                                 tttGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGaa                                                  >  1:302070/1‑250 (MQ=255)                                                                                                                                                                                                          acatacccATCTGACTGTTGGGGGCCTGGGGGAAAAAACCCGCCGGTATTGTCAGGTATAACGATGTGCCAACAAGGGTAAACGAGTAGCCTGGCGGGATGACCCGCCCCACCACCCATTTACGGCAGCCGAGTTTCTCCATCTTGGCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCt                                        <  1:172997/245‑1 (MQ=255)                                                                                                                                                                                                                                      ggcaaTAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGcacca            <  2:43814/247‑1 (MQ=255)                                                                                                                                                                                                                                          aaaagcacCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACaaa        <  2:6073/246‑1 (MQ=255)                                                                                                                                                                                                                                                acCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGAAGCCTGCCGGGATGACCAGACCCACCACCGCTTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATAAGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGAt  >  2:18508/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGAT  >  NC_000913/3682268‑3682757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑