Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,443,188	T→C	T304A (ACA→GCA)	secY ←	Sec translocon subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,188	0	T	C	100.0%	103.0 / NA	31	T304A (ACA→GCA)	secY	Sec translocon subunit SecY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/11);  total (20/11)

TAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTGCAAC  >  NC_000913/3442948‑3443431                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    tAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTc                                                                                                                                                                                                                                           >  2:57812/1‑251 (MQ=255)           ataCAGCGCACCAACCAGGGTCAGGCGGGGCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTTGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTAc                                                                                                                                                                                                                                 <  2:278463/251‑1 (MQ=255)             aCAGCGCACCAACCAGGGTCAGGCGGGTCATTCCTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGCCTTCTTCAGGTTATCTGCTGTTTCACGCTGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCg                                                                                                                                                                                                                               >  1:303266/1‑251 (MQ=255)                    accaaccaGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCg                                                                                                                                                                                                                         >  1:327233/1‑250 (MQ=255)                                         cATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGCCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCg                                                                                                                                                                                                   >  1:246722/1‑251 (MQ=255)                                            tACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCggg                                                                                                                                                                                                 >  2:110007/1‑250 (MQ=255)                                               tttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGCCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCAAGCCAGt                                                                                                                                                                                                                                             >  2:145732/1‑203 (MQ=255)                                               tttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGt                                                                                                                                                                                                                                             <  1:145732/203‑1 (MQ=255)                                                ttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACa                                                                                                                                                                                             <  2:45464/250‑1 (MQ=255)                                                  aTCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGa                                                                                                                                                                                           >  1:61898/1‑250 (MQ=255)                                                    cGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGaat                                                                                                                                                                                         <  1:137179/250‑1 (MQ=255)                                                     gATATCCTTTGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGCCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGACGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGaata                                                                                                                                                                                        >  2:27646/1‑250 (MQ=255)                                                                ccGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCg                                                                                                                                                                            <  1:288536/251‑1 (MQ=255)                                                                              ggACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGAt                                                                                                                                                              <  1:200750/251‑1 (MQ=255)                                                                                  gAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGATTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                                                                          <  2:257384/251‑1 (MQ=255)                                                                                   aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccc                                                                                                                                                         <  2:327233/251‑1 (MQ=255)                                                                                        cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                                    >  1:169859/1‑251 (MQ=255)                                                                                                                       aTCTCCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCACCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                     >  1:148423/1‑251 (MQ=255)                                                                                                                                tccACGCGG‑TTGAAAAACCAACGCCGTGTAGAAAAAACAAAAAAAGATGATGGCAGCCGCATAGAGTAACAAAAAACGCGGTTGCCCAGGCGGAAAATACAGCGAAATTGTGCCCAGCCAGTCCCACCCATTCCCCCCCCCGACCCAGGACGGGATGTGCGCGGGAAACAAAATAATACTGGAAGCAAATTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                             <  1:220357/248‑1 (MQ=255)                                                                                                                                                         ggtgaaaaaaaaaCAAAAGAAGATGATTGCAGACGCATAGAGTAACAAAAAAGGCGGTTGCCCAGGCTAAAAATACAGCAAAATTGTTGCCAGCCATTTCCAACCAGTCCCCCCCCCGAACCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                    <  1:110007/241‑1 (MQ=255)                                                                                                                                                         cGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                    >  2:212007/1‑251 (MQ=255)                                                                                                                                                          gtgtAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGTCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                                   >  2:252273/1‑251 (MQ=255)                                                                                                                                                               gaagaaACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                              >  2:80912/1‑251 (MQ=255)                                                                                                                                                                     aCAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCACGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                         >  1:370896/1‑250 (MQ=255)                                                                                                                                                                          agaagaTGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                    >  1:129948/1‑250 (MQ=255)                                                                                                                                                                                tgatTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGcc                                                                                                                                                                                                                             >  2:171286/1‑91 (MQ=255)                                                                                                                                                                                tgatTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGcc                                                                                                                                                                                                                             <  1:171286/91‑1 (MQ=255)                                                                                                                                                                                                                        aGGCTGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                     >  2:116060/1‑251 (MQ=255)                                                                                                                                                                                                                            tGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                                   >  2:122332/1‑185 (MQ=255)                                                                                                                                                                                                                            tGCAAATACAGCGAAATTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa                  >  2:82836/1‑250 (MQ=255)                                                                                                                                                                                                                                           aTTGTTGCCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTGcaac  >  1:148919/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    TAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAA‑CCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTGCAAC  >  NC_000913/3442948‑3443431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑