Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,697	G→A	E60E (GAG→GAA)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,697	0	G	A	100.0%	133.9 / NA	41	E60E (GAG→GAA)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/12);  total (29/12)

AGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCG  >  NC_000913/3436456‑3436941                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGcc                                                                                                                                                                                                                                             <  1:69565/251‑1 (MQ=255) agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGGGcc                                                                                                                                                                                                                                             >  2:315446/1‑251 (MQ=255)     cgaaggCTTCTTTTACGCTAAGCTAATCAAAAAGTGAGGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAAAACAACGATATTACTGTTGTAGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAGTGCGGGAAGCAGGTGCcgccg                                                                                                                                                                                                                                         <  2:201503/247‑4 (MQ=255)        cGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGacgacg                                                                                                                                                                                                                                      >  1:331879/1‑251 (MQ=255)            ttcttTTACGCTAAGCTAATCAAACAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCg                                                                                                                                                                                                                                   >  1:126898/1‑250 (MQ=255)                  tACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGGGCCGACGACGCCGATATGCt                                                                                                                                                                                                                            >  2:62731/1‑251 (MQ=255)                          gctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGAGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTg                                                                                                                                                                                                                    >  1:249566/1‑251 (MQ=255)                             aaTCAAAAAGTGAGGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGTCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACTATATTACTGTTGTCGAAACCAACGGTGAGCGTCTGCGTACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCta                                                                                                                                                                                                                    <  2:252264/248‑2 (MQ=255)                                 aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAAcc                                                                                                                                                                                                               >  1:246949/1‑249 (MQ=255)                                     aGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCa                                                                                                                                                                                                         <  1:11025/251‑1 (MQ=255)                                      gTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAg                                                                                                                                                                                                        <  1:241329/251‑1 (MQ=255)                                                        gcgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtg                                                                                                                                                                                      >  2:310783/1‑251 (MQ=255)                                                           aCTGTTGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCCCATTGCGGGAAGCAGGTGCCGACGACGCCGTTTTGCTGGTTGATGTAACCAGTTTAAATGAAACCAATATGGTtgcct                                                                                                                                                                                   >  2:69133/1‑251 (MQ=255)                                                              gatgaAACTTATCATTCTGGGTGCCGGCCAGGATGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgcc                                                                                                                                                                                >  1:287575/1‑251 (MQ=255)                                                              gatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgcc                                                                                                                                                                                >  1:70211/1‑251 (MQ=255)                                                              gatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctg                                                                                                                                                                                  <  1:315446/249‑1 (MQ=255)                                                                              cTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACt                                                                                                                                                                >  1:71992/1‑251 (MQ=255)                                                                                 ggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACtttt                                                                                                                                                             >  1:221719/1‑251 (MQ=255)                                                                                       ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAacac                                                                                                                                                       <  1:295639/251‑1 (MQ=255)                                                                                       ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAacac                                                                                                                                                       >  1:202212/1‑251 (MQ=255)                                                                                         ccAGGTTGGAGGCACACTGGCGGAAAAACTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGATCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGGTGCCTGCCAGGTAGCCTACTCACTTTTCAACAccc                                                                                                                                                     >  2:341420/1‑251 (MQ=255)                                                                                             gTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGTACCTTACAGGATAAAGTTGACCTGCGGGTCGTGCAGGGGCATGTCTCTCATCCACGCGTATTTCGGGAAGCAGGTGCCGACGACGCCGAGATGCTGCTTGCTGTAGCCAGTTCAGATGAAACCAATATGGGTGCCGGTGAGGTAGCCTACGCACTTTTCAACACCCCTaa                                                                                                                                                 <  2:35057/251‑1 (MQ=255)                                                                                                  cggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAatcgc                                                                                                                                             >  2:74821/1‑250 (MQ=255)                                                                                                             cGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGGTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATc                                                                                                                                 <  2:49534/251‑1 (MQ=255)                                                                                                                               agaACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTg                                                                                                               >  2:285765/1‑251 (MQ=255)                                                                                                                                    aacGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGcgcg                                                                                                           >  1:92813/1‑250 (MQ=255)                                                                                                                                              cTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGGTAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGATGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATa                                                                                                  >  1:169234/1‑249 (MQ=255)                                                                                                                                                      gATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttc                                                                                         >  2:313780/1‑250 (MQ=255)                                                                                                                                                         aCCAACGGTGAGCTTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCCTCCACGCGTATTGCGGGCAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAACCCAATCTGGTTGCCTGCCCGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCTCTCACCAGACTCCGTGCGCGATGCCGATAATCTATttcattc                                                                                     >  2:221165/1‑251 (MQ=255)                                                                                                                                                               ggTGAGCGTCTGCGGACCTTACAGGATAAATATGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGc                                                                               >  2:103638/1‑251 (MQ=255)                                                                                                                                                                gTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGTATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCt                                                                              >  1:288752/1‑251 (MQ=255)                                                                                                                                                                                  tACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTg                                                            >  2:123228/1‑251 (MQ=255)                                                                                                                                                                                    cAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGAt                                                          >  1:42177/1‑251 (MQ=255)                                                                                                                                                                                     aGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATc                                                         >  1:4662/1‑251 (MQ=255)                                                                                                                                                                                                     gCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCGCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATGTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTgg                                         <  2:40771/251‑1 (MQ=255)                                                                                                                                                                                                        ggTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGtt                                       >  1:166561/1‑250 (MQ=255)                                                                                                                                                                                                           cGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATc                                    <  1:66359/250‑1 (MQ=255)                                                                                                                                                                                                                 ggggCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGataat                              >  2:107786/1‑250 (MQ=255)                                                                                                                                                                                                                   ggCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATAtt                           >  1:291061/1‑251 (MQ=255)                                                                                                                                                                                                                                 caaCGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGACTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTg              >  1:53509/3‑250 (MQ=255)                                                                                                                                                                                                                                            gCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGgcg  <  2:152384/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     AGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCG  >  NC_000913/3436456‑3436941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑