Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,432	A→G	E190G (GAA→GGA)	acrE →	multidrug efflux pump membrane fusion lipoprotein AcrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,432	0	A	G	100.0%	146.5 / NA	45	E190G (GAA→GGA)	acrE	multidrug efflux pump membrane fusion lipoprotein AcrE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

AAAGGCGAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAA  >  NC_000913/3414185‑3414668                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             aaaGGCGAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAgg                                                                                                                                                                                                                                           <  1:8319/251‑1 (MQ=255)      cGAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGctc                                                                                                                                                                                                                                      >  1:295822/1‑251 (MQ=255)       gAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGCCGTATCGGCAAATCGACTGG‑GACCGGAGGCGctct                                                                                                                                                                                                                                     >  2:293710/1‑251 (MQ=255)                         gccgccgccgccATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAAc                                                                                                                                                                                                                   <  2:208816/251‑1 (MQ=255)                                ccgccATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGACGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGAATGa                                                                                                                                                                                                             >  1:133835/1‑250 (MQ=255)                                  gccATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACt                                                                                                                                                                                                          <  2:69018/251‑1 (MQ=255)                                     aTCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGc                                                                                                                                                                                                       >  1:203087/1‑251 (MQ=255)                                                         acgttacgtgCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATc                                                                                                                                                                                   <  2:267741/251‑1 (MQ=255)                                                             tccgttCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTa                                                                                                                                                                                <  2:285600/248‑1 (MQ=255)                                                                  tCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTAc                                                                                                                                                                           <  1:94670/250‑1 (MQ=255)                                                                           gggTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAtgtg                                                                                                                                                                  >  1:355779/1‑250 (MQ=255)                                                                           gggTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGa                                                                                                                                                                 <  1:264988/251‑1 (MQ=255)                                                                                 gAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCACAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATc                                                                                                                                                                                   >  2:181903/1‑227 (MQ=255)                                                                                 gAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATc                                                                                                                                                                                   <  1:181903/227‑1 (MQ=255)                                                                                              cgtcagGAGTACGACCAGGCCAGTGCTGATGCGCGCCAGGCCGATGCCGCCGTGTTGGCCGCAAAAGCCACAGGCGAAAGCTCTCTCATCAATCTTGCTTATACCAAATTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑TACCTGAGGCGCGCTTTTCTCTAATTGGCAAACGACTTAACGGTCGACGGTCTAGCAGCTAGATCCTAGCTACGTTGATGGGACCCAATCCAGCatggattt                                                                                                                                              <  2:172878/248‑8 (MQ=255)                                                                                                      gTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGc                                                                                                                                      >  2:126475/1‑251 (MQ=255)                                                                                                          gACCAGGCAATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGGATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATTGGCAAACGACTGAACGGGCGACTGTCCAGCAGCTCGGTCCTATCTACGTTGATGTGACCCAAGCCAGCAACGATTTTATGAGGCTGaa                                                                                                                                  <  2:38200/251‑1 (MQ=255)                                                                                                               ggCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAt                                                                                                                             >  1:231850/1‑251 (MQ=255)                                                                                                                gCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                                                                                            <  2:265615/251‑1 (MQ=255)                                                                                                                gCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                                                                                            >  1:13664/1‑251 (MQ=255)                                                                                                                      gCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTag                                                                                                                       <  1:195860/250‑1 (MQ=255)                                                                                                                         gATGCTCGTCAGGCCGATGCCTCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTagag                                                                                                                     <  1:137831/249‑1 (MQ=255)                                                                                                                            gcgcGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAgg                                                                                                                <  2:132146/248‑1 (MQ=255)                                                                                                                            gcacGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAgg                                                                                                                <  2:146244/248‑1 (MQ=255)                                                                                                                                gTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAt                                                                                                            >  1:120152/1‑251 (MQ=255)                                                                                                                                   aGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTg                                                                                                         >  2:211218/1‑251 (MQ=255)                                                                                                                                         aTGCCGCCGTGATTGACGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCACGGAAATTTGCATa                                                                                                     >  2:35708/1‑249 (MQ=255)                                                                                                                                              gccgTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGaaa                                                                                               >  1:248040/1‑250 (MQ=255)                                                                                                                                                             aaaGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGt                                                                               >  1:359904/1‑251 (MQ=255)                                                                                                                                                                 ccACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGAATGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTag                                                                             >  1:221107/1‑249 (MQ=255)                                                                                                                                                                                  cGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACg                                                           <  2:231850/250‑1 (MQ=255)                                                                                                                                                                                  cGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACg                                                           <  2:32162/250‑1 (MQ=255)                                                                                                                                                                                  cGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAAGGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTTGTCATGGAAAAc                                                            <  1:313788/249‑1 (MQ=255)                                                                                                                                                                                       cAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAACCGGTCaa                                                      >  2:50274/1‑250 (MQ=255)                                                                                                                                                                                          tCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt                                                  >  1:271196/1‑251 (MQ=255)                                                                                                                                                                                                                          gCGGAAGTATCGTCAAAGCGA‑TGTTGACCGGAGGCGCTCTTGTCACTAATGGGTAAACGACTGAACTGGCGACGGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCGAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCATACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAt                  <  2:191695/251‑1 (MQ=255)                                                                                                                                                                                                                           cGGACGTATCGGCAAATCGACTGT‑GACCGGAGGAGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACGCAATACAGCAACGATTTTATGAGGCTGAAGCAATAAGTAGAGCAAGGAAATTTGCATAAGGAAAACGCAACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAAACTATCACCTGAAAGGTACGCTGCAATTCTCCGAtg                 >  1:207134/1‑251 (MQ=255)                                                                                                                                                                                                                           cGGACGTATCGGCAAATCGACTGT‑GACAGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGGAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGTTACGCAGCAATTCTCCGAtg                 >  1:207133/1‑251 (MQ=255)                                                                                                                                                                                                                            ggACGTATCGGCAAATAGACTGT‑GACCGGAGGCGCTCTTGTCACTAACGGGACAACGACCGAACCGGCGACTGTCCAGCAGCTAGATCCTATATACGTTGATGCGACCCAATCCAGCAACGCTTTTATGCGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATACCCTGAAAGGTACGCTGCAATTCTCCGAtgg                >  1:217859/1‑250 (MQ=255)                                                                                                                                                                                                                             gACGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgtg               >  1:124854/1‑251 (MQ=255)                                                                                                                                                                                                                              aCGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                       >  1:100183/1‑242 (MQ=255)                                                                                                                                                                                                                              aCGTATCGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                       <  2:100183/242‑1 (MQ=255)                                                                                                                                                                                                                                    cGGCAAATCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGtt         <  1:344310/250‑1 (MQ=255)                                                                                                                                                                                                                                         aaTCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGCAAGGTACGCTGCAATTCTCCGATGTGACCGTtgatga    >  1:60713/1‑250 (MQ=255)                                                                                                                                                                                                                                          aTCGACTGT‑GACCGGAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACGGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaaa  <  1:287133/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             AAAGGCGAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTACGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGTCAGGCCGATGCCGCCGTGATTGCCGCAAAAGCCACAGTCGAAAGCGCTCGCATCAATCTTGCTTATACCAAAGTCACTGCGCCAATTAGCGGACGTATCGGCAAATCGACTGT‑GACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAA  >  NC_000913/3414185‑3414668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑