Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	166.7 / NA	52	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/19);  total (33/19)

GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGC  >  NC_000913/3387799‑3388286                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        gATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAACAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcggcgg                                                                                                                                                                                                                                                 >  2:178919/1‑251 (MQ=255)      gcgcGGTATAACCGGCTAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCTGATCATCGCAATGATTATCACCGGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAGTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCTGCGGcaaca                                                                                                                                                                                                                                            <  1:60790/251‑1 (MQ=255)             ataACCGGCCAGCCCCCACGCATCCGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcgcc                                                                                                                                                                                                                                      <  2:245603/250‑1 (MQ=255)             ataACCGGCCAGCCCCCACGCATACGCGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcg                                                                                                                                                                                                                                        >  1:105042/1‑248 (MQ=255)             ataACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcg                                                                                                                                                                                                                                        <  2:105042/248‑1 (MQ=255)                            ccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGccc                                                                                                                                                                                                                      >  1:283339/1‑251 (MQ=255)                              aCGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGccc                                                                                                                                                                                                                      >  1:326274/1‑249 (MQ=255)                               cGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGCTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGc                                                                                                                                                                                                                   >  2:264863/1‑251 (MQ=255)                                              tATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCt                                                                                                                                                                                                    >  1:76660/1‑251 (MQ=255)                                                     aCCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTg                                                                                                                                                                                             <  1:196728/251‑1 (MQ=255)                                                     aCCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAAGGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGt                                                                                                                                                                                              <  2:274521/250‑1 (MQ=255)                                                             ggATATCCCGGCACAAAAACAGGCCAAGATACCGCCCACCAGAATCATCAATAATGATGCGCGGACCATCGCAATGATGATCACCAGACCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCCGCGTGGCGTTTCCAGCTGGAAGTGCAAGCca                                                                                                                                                                                      >  2:220973/1‑250 (MQ=255)                                                              gATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCCGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaac                                                                                                                                                                                    >  1:269578/1‑251 (MQ=255)                                                               atatCCAGGTACAAAAACCGGACCAGATACAGCACACCAGAATCATCAATAATGGCGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaac                                                                                                                                                                                    >  2:309733/1‑250 (MQ=255)                                                                                ccGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAt                                                                                                                                                                  <  2:40342/251‑1 (MQ=255)                                                                                       aGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTc                                                                                                                                                           >  1:340071/1‑251 (MQ=255)                                                                                         aTACCGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGTCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGCATACGGTTCACCTCCCGCAGCAAAGTCCGGACCGGCGGCCACAATCGCCGCTGTCAGTACCGCCCAGCGTTGCGTTTCCAGCTGGAAGTGAAAGCCAACAACCAGCCCCCGTACCATGTCGGgcgc                                                                                                                                                         >  2:330837/1‑251 (MQ=255)                                                                                          tACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGcc                                                                                                                                                        >  2:132628/1‑251 (MQ=255)                                                                                              gCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCCATGATGATCACCAGTCCGGCAATACAGCCAATACATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGACAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGACAGTACAATGGCGGTCGCCAggt                                                                                                                                                    >  1:28008/1‑249 (MQ=255)                                                                                               cacaCCATAAGCATCAATAATGGTGCGCGGATCATCGCATTTATGATCCCAAGACGGGAAATACAGCCAATAAATGGGACTATGATGCGCAAAAAGCCACGATAGCGAATAGCTCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCATCTGGAAGTTAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGttt                                                                                                                                                   <  2:243591/251‑1 (MQ=255)                                                                                                             caATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGa                                                                                                                                     <  1:242905/250‑1 (MQ=255)                                                                                                                   tGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAACGAATATGtt                                                                                                                              >  1:1885/1‑251 (MQ=255)                                                                                                                    ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGtt                                                                                                                              <  1:364133/250‑1 (MQ=255)                                                                                                                         gcgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAg                                                                                                                        <  2:340071/251‑1 (MQ=255)                                                                                                                                            atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAGCAGCGCCAGTACaa                                                                                                                                                                   <  1:65806/190‑1 (MQ=255)                                                                                                                                            atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACaa                                                                                                                                                                   >  2:65806/1‑190 (MQ=255)                                                                                                                                            atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTAACGCA‑AAGCGAATATGTTGGTTAGCAATGGGGAAAATAcccct                                                                                                     >  1:373881/1‑249 (MQ=255)                                                                                                                                            atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCAt                                                                                                     <  1:221129/251‑1 (MQ=255)                                                                                                                                               aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCCCA‑AAGCGGATATGTTGGTTTGCAATGGGGAAAATAACCATccg                                                                                                  >  2:50335/1‑249 (MQ=255)                                                                                                                                                  aGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGGGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑t                                                                                              >  1:205008/1‑251 (MQ=255)                                                                                                                                                       ggCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCA‑AAGCGAATATGTTGGTTAGGAATGGAGAAAATAACCATCGTGA‑TTAAcc                                                                                         >  1:4281/1‑251 (MQ=255)                                                                                                                                                          aaTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCAt                                                                                                     >  1:52990/1‑237 (MQ=255)                                                                                                                                                          aaTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCAt                                                                                                     <  2:52990/237‑1 (MQ=255)                                                                                                                                                          aaTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCaaa                                                                                      <  2:58539/251‑1 (MQ=255)                                                                                                                                                                  cAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCaaaaccacgc                                                                               >  1:191291/1‑243 (MQ=255)                                                                                                                                                                  cAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGGTAGCAAAGGAGAAAATACCCCTCGTGA‑TTAACCAAACTCCCGcc                                                                              >  2:117299/1‑250 (MQ=255)                                                                                                                                                                       aaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTTCCGCACAACCGA‑TCTGTTGGTTAGCAAAGGAGAAAAAACCCCTCGTGA‑TTAACCAAACTTACGCCGGCgg                                                                         >  2:111199/1‑250 (MQ=255)                                                                                                                                                                             ccGATGATGCGCAAAACGCCACGATAGCGAATAGCCCCAGACTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGG‑TTTACCAAAATCCCGCAGGCGATGGGcc                                                                   >  1:209965/1‑251 (MQ=255)                                                                                                                                                                             ccGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGcc                                                                   <  2:76660/251‑1 (MQ=255)                                                                                                                                                                                     gcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACg                                                           <  1:292141/251‑1 (MQ=255)                                                                                                                                                                                      cgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGAGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGGCAGTTTTACCCCA‑AAGCGGATATGTTGGGTAGTAACGGCGAAAATACCCATTG‑GACTTTACCAAACTCACGCAGGGGAGTGGCCATTTTACgg                                                          >  1:200740/1‑251 (MQ=255)                                                                                                                                                                                        cAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGga                                                         >  1:109707/1‑250 (MQ=255)                                                                                                                                                                                            aaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAAc                                                    >  1:226805/1‑251 (MQ=255)                                                                                                                                                                                                   gATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCA‑AAGCCAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTAAGGAAGAACGAATCCt                                             >  1:26021/1‑251 (MQ=255)                                                                                                                                                                                                       gCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCt                                         <  1:261860/251‑1 (MQ=255)                                                                                                                                                                                                                gcCAGAATACGGTGCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACGATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCTTGA‑GTAACCAAACTCACGCAGGCGATTGGCCATTTTACGGAAGAACGAATCCTGTCTTTCGTcgcg                                <  2:31906/251‑1 (MQ=255)                                                                                                                                                                                                                      aTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCC‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTTA‑TTAACCAAACCCACGCCGGCGATTGGCCATTTTACGGAAGAACGAATCCCGGCTTTCGTCGCGATCttt                          >  2:118775/1‑250 (MQ=255)                                                                                                                                                                                                                           gTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAAAATGTTGGTTAGCAATGGAGAAAATACCCATCGGGA‑TTAACCCAAATCACGCAGGCCATGGGTCCTTTTACGGAAAAATGAATCCTGGCTTTCGTCCGGATCTTGTTTGc                     >  2:109200/1‑251 (MQ=255)                                                                                                                                                                                                                                ccTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCGCC‑AAGCGAATATGTTGGTTTGCAATGGAGAAAAAACCCATCGTGA‑TTAACCAAACTCACGCAGGCGGTGGGGCATTTTACCGAAGAACGAATCCCGGCTTTCGTCGCGCTCTTGTTTGCCAGTg                >  2:117491/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCTAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTa                                                                                            <  2:189535/174‑1 (MQ=255)                                                                                                                                                                                                                                 cTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTa                                                                                            >  1:189535/1‑174 (MQ=255)                                                                                                                                                                                                                                               gCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGGTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCCGTGACCACCACTGTAGc  >  1:373684/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCA‑AAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGA‑TTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGC  >  NC_000913/3387799‑3388286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑