Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,121	T→C	V418A (GTC→GCC)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,121	0	T	C	100.0%	136.1 / NA	41	V418A (GTC→GCC)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

CGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCA  >  NC_000913/3375876‑3376354                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          cGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTa                                                                                                                                                                                                                                      <  1:311415/251‑1 (MQ=255)     gCGGTTATCAGGGGGGCGGAGCAGTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTAGGCCGATGCATTTTGCCTCGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGt                                                                                                                                                                                                                                   <  1:235725/250‑1 (MQ=255)                      ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATTATTATGCCGATGCATTTTGCCCCGACTCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGttt                                                                                                                                                                                                                 >  2:31105/1‑251 (MQ=255)                          cATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGcc                                                                                                                                                                                                             <  1:293200/251‑1 (MQ=255)                             tctggtctggGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCttt                                                                                                                                                                                                          >  1:232804/1‑251 (MQ=255)                                        cGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGGGGCCGTTACGTCAGGGATTGCAGGCGTTTCGCCTTTTGCGgtggtg                                                                                                                                                                                                >  2:62111/1‑250 (MQ=255)                                              tGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCgg                                                                                                                                                                                          <  2:119553/250‑1 (MQ=255)                                                    gatgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCCGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCg                                                                                                                                                                                    >  2:7666/1‑250 (MQ=255)                                                          tattatGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATcccc                                                                                                                                                                              <  2:201674/250‑1 (MQ=255)                                                          tattatGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATcccc                                                                                                                                                                              <  1:57878/250‑1 (MQ=255)                                                                      ggTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGt                                                                                                                                                                  <  1:312175/250‑1 (MQ=255)                                                                       gTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGttt                                                                                                                                                                <  2:215763/251‑1 (MQ=255)                                                                         cATTGCCATTTGTGCCATTGTGATGGTCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGACTATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGAGGTGGGGGCCGTTACGTCAGGCATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGGCAGCGATCCCCATGGCCGTCGGttt                                                                                                                                                                >  2:197705/1‑249 (MQ=255)                                                                         cATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGAGTTTCGCCTTTTGCGGTGGTGAAGCGGAAAGCGAACCCCATGGCAGTCGGTTTCg                                                                                                                                                              >  2:290882/1‑251 (MQ=255)                                                                                aTTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGa                                                                                                                                                        >  1:38991/1‑250 (MQ=255)                                                                                        cATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTg                                                                                                                                               <  1:287544/251‑1 (MQ=255)                                                                                               aTGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAt                                                                                                                                        <  1:119911/251‑1 (MQ=255)                                                                                                        ggCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCCGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGCCGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAtt                                                                                                                               <  2:84442/251‑1 (MQ=255)                                                                                                        ggCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTa                                                                                                                                 <  2:148246/249‑1 (MQ=255)                                                                                                                 ccGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGt                                                                                                                      <  2:38991/251‑1 (MQ=255)                                                                                                                    tttATGTCATTTGCCAGACTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGAGCGCGCGGATTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTAGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACACTCACGCTATTTTATTAAGTCAt                                                                                                                   >  1:132160/1‑251 (MQ=255)                                                                                                                           cATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTaaaa                                                                                                              >  1:262634/1‑249 (MQ=255)                                                                                                                                    gTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAAc                                                                                                     >  1:47370/1‑249 (MQ=255)                                                                                                                                          ttCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCg                                                                                                                                                                                    <  2:138777/164‑1 (MQ=255)                                                                                                                                          ttCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCg                                                                                                                                                                                    >  1:138777/1‑164 (MQ=255)                                                                                                                                             cGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTAAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGGTTCGTGGCGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                                                          >  1:145690/1‑251 (MQ=255)                                                                                                                                                atatCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGcc                                                                                       <  2:328240/251‑1 (MQ=255)                                                                                                                                                  atCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCt                                                                                      <  1:132298/250‑1 (MQ=255)                                                                                                                                                      cAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGtttt                                                                                 >  1:225707/1‑251 (MQ=255)                                                                                                                                                                        cAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTaacaacccg                                                               >  2:11068/1‑248 (MQ=255)                                                                                                                                                                                              gATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACcgcgg                                         >  2:134763/1‑250 (MQ=255)                                                                                                                                                                                               aTGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgtg                                        <  1:15936/251‑1 (MQ=255)                                                                                                                                                                                                   aTTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTc                                                                                                                                                                     <  1:58205/122‑1 (MQ=255)                                                                                                                                                                                                   aTTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTc                                                                                                                                                                     >  2:58205/1‑122 (MQ=255)                                                                                                                                                                                                     tttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCt                                  >  2:335089/1‑251 (MQ=255)                                                                                                                                                                                                              acgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGtt                         <  1:356075/251‑1 (MQ=255)                                                                                                                                                                                                                         cgcgGTTTCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAgg               >  2:251042/1‑250 (MQ=255)                                                                                                                                                                                                                              tttCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACCGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACa                                                                                                    >  2:186497/1‑160 (MQ=255)                                                                                                                                                                                                                              tttCGCCGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACa                                                                                                    <  1:186497/160‑1 (MQ=255)                                                                                                                                                                                                                                    cGATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGtcatc   <  1:205010/251‑1 (MQ=255)                                                                                                                                                                                                                                     gATTACTGCGGTGGTGGCCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGtcatcc  >  1:122305/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          CGATGCGGTTATCAGGGGGGCGGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCA  >  NC_000913/3375876‑3376354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑