Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,262,907	T→C	T252A (ACC→GCC)	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,262,907	0	T	C	100.0%	119.1 / NA	35	T252A (ACC→GCC)	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

TGAATTCTCTCCTATTCCGCCGGTGAATATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCAT  >  NC_000913/3262668‑3263146                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                tGAATTCTCTCCTATTCCGCCGGTGAATATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTtggctggc                                                                                                                                                                                                                                        <  1:280685/249‑1 (MQ=255)                           atatAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGAc                                                                                                                                                                                                             >  2:251246/1‑250 (MQ=255)                             ataATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATc                                                                                                                                                                                                          <  1:31933/251‑1 (MQ=255)                                aTTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                                                                                                       >  2:134241/1‑251 (MQ=255)                                       ccaGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCgg                                                                                                                                                                                                <  2:186027/251‑1 (MQ=255)                                            cGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGCCATCGCACCAAAGTCGACATCGCCCCTGCGGGTAcc                                                                                                                                                                                           >  2:361882/1‑251 (MQ=255)                                                 tAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCcatc                                                                                                                                                                                       >  2:185901/1‑250 (MQ=255)                                                      aaGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCAtcatca                                                                                                                                                                                   >  2:13515/1‑249 (MQ=255)                                                             gtgtCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTTGTTGGCTTGGCTGGCGACCCAGGACAGCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCa                                                                                                                                                                          <  2:250852/251‑1 (MQ=255)                                                               gtCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAg                                                                                                                                                                         <  1:185901/250‑1 (MQ=255)                                                               gtCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGACGACATCGCCACTGCGGGTACCCATCATCAAGACTTCCAgc                                                                                                                                                                        >  2:270699/1‑251 (MQ=255)                                                                                              aaaGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACActct                                                                                                                                         <  1:132546/251‑1 (MQ=255)                                                                                               aaGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTg                                                                                                                                        >  1:212221/1‑251 (MQ=255)                                                                                                       aaTTGCCAGTTGCGCGCGTTCGTGACCGTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTg                                                                                                                                 <  1:341758/250‑1 (MQ=255)                                                                                                                      gcgTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTg                                                                                                                 <  2:22260/251‑1 (MQ=255)                                                                                                                       cgTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGGCTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTg                                                                                                                 >  2:185292/1‑250 (MQ=255)                                                                                                                       cgTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCGTCCAGCGGCGTCATTCCCATTGAGGGATCAACACTCTGACCGTTGCGAACCGCGCAGATTGa                                                                                                                <  2:353251/251‑1 (MQ=255)                                                                                                                                       gCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAg                                                                                                 <  1:49016/250‑1 (MQ=255)                                                                                                                                                tttCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCcac                                                                                        >  1:55526/1‑250 (MQ=255)                                                                                                                                                  tCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAAc                                                                                     >  1:249567/1‑251 (MQ=255)                                                                                                                                                   ccAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAAc                                                                                     <  1:241911/250‑1 (MQ=255)                                                                                                                                                              aaaTCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTc                                                                         <  2:173201/251‑1 (MQ=255)                                                                                                                                                                    gaagaaAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAACGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTc                                                                   >  2:144388/1‑251 (MQ=255)                                                                                                                                                                      agaaagaCCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCg                                                                 >  1:46713/1‑251 (MQ=255)                                                                                                                                                                            aCCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGcc                                                               >  2:356426/1‑247 (MQ=255)                                                                                                                                                                                           taatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGCTTCAGCAGCGAAtgtg                                            >  2:340470/1‑251 (MQ=255)                                                                                                                                                                                                ccGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTgcgcgc                                       >  1:55966/1‑251 (MQ=255)                                                                                                                                                                                                 cGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACTCTCTGACCGTTGCGAACCGCGCAGATTGACGCTCCATTGCCAATATGCGCCACAGCCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCt                                      <  1:228552/251‑1 (MQ=255)                                                                                                                                                                                                        ttATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGaa                                 <  2:249567/249‑1 (MQ=255)                                                                                                                                                                                                                 aCGCGTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTg                      >  2:310160/1‑251 (MQ=255)                                                                                                                                                                                                                    cgTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAACGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTcc                                                                                                                                                             >  2:157423/1‑113 (MQ=255)                                                                                                                                                                                                                    cgTTCCAGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTcc                                                                                                                                                             <  1:157423/113‑1 (MQ=255)                                                                                                                                                                                                                          aGGTCACCCAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGcc             >  1:317346/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccAGGCTCTGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCCGGGAAACATAGCGGTGCGACGTGCCGTGGaa       >  2:255958/1‑250 (MQ=255)                                                                                                                                                                                                                                      ctctGGTTGGCTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCAt  >  1:269494/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                TGAATTCTCTCCTATTCCGCCGGTGAATATAATTCCATCCAGGCGACGTAATGAAGCTGCGTGTCCGGCAATATGACGGGCAATTCGGTGAACAAAGGTTTTAATTGCCAGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCAT  >  NC_000913/3262668‑3263146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑