Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,712	T→C	I169T (ATC→ACC)	ygiM →	putative signal transduction protein (SH3 domain)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,712	0	T	C	100.0%	130.5 / NA	40	I169T (ATC→ACC)	ygiM	putative signal transduction protein (SH3 domain)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/11);  total (29/11)

CACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCA  >  NC_000913/3201464‑3201948                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               cACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAA‑GGGGAGTCGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAAACAGAAACTGAAAAAAGAGCTGATTGTCGCGCAGAAAAAGGTCGATGGCGCCAGCGTAAAGCTGGATGAAAAACAGGGCACcaccat                                                                                                                                                                                                                                             >  1:263524/1‑251 (MQ=255)     gAGCCAAGCCTGCGCTTCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACcaccatcat                                                                                                                                                                                                                                          >  1:338189/1‑251 (MQ=255)                              cAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTggg                                                                                                                                                                                                                 >  2:293108/1‑250 (MQ=255)                                gATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCgg                                                                                                                                                                                                               >  2:170984/1‑250 (MQ=255)                                     ggAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTgg                                                                                                                                                                                                          <  1:121307/251‑1 (MQ=255)                                            cAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGCATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGggcttgg                                                                                                                                                                                                   >  1:215094/1‑251 (MQ=255)                                             aGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTTCTGGggcttggc                                                                                                                                                                                                  <  1:307681/251‑1 (MQ=255)                                                  aaaaCCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTtgct                                                                                                                                                                                             >  1:146741/1‑251 (MQ=255)                                                               aTAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGAGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCttgt                                                                                                                                                                                 >  2:168981/1‑247 (MQ=255)                                                                tAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGGGTGTTGGGGCTTGGCTTTCTGCTCGGTCTGGTAc                                                                                                                                                                               >  1:33568/1‑251 (MQ=255)                                                                 aaaCTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAAAGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGATTTGCTTGCTGGTCGGTCTGGGACt                                                                                                                                                                              >  1:75105/1‑251 (MQ=255)                                                                            tatCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCCCCTg                                                                                                                                                                    >  1:297657/1‑250 (MQ=255)                                                                                    acacCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGc                                                                                                                                                           >  1:118960/1‑251 (MQ=255)                                                                                       ccTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGGGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgc                                                                                                                                                        >  1:155372/1‑251 (MQ=255)                                                                                           gAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTTGGGCTTTGCTTGCTGGTCGGTTTGGTACTGCCGCCCCTTGTCCCAAggacccaac                                                                                                                                                    >  2:248026/1‑243 (MQ=255)                                                                                           gAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGGGGGGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgcaaac                                                                                                                                                    >  1:299270/1‑251 (MQ=255)                                                                                             aTCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGGGGCCTGCTGTGGCTTTGCTTGCTGTTCGGTCTGGTTCTTCCGCACCTGCTGCCAcgccgccaaacc                                                                                                                                                  >  2:202906/1‑245 (MQ=255)                                                                                                     tccGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAACAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACAACCATCATGCCATGGTTTATGTATGGCGGTGGCGGTCTGGGGCTTGGCTTGCTGTCCTGTCTGGAACTGCCGCACCTTATCCCAAGCCGCAAAAGCAAAGAt                                                                                                                                            >  2:315286/2‑249 (MQ=255)                                                                                                       cGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCt                                                                                                                                        >  1:300361/1‑251 (MQ=255)                                                                                                        gCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGAGTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTg                                                                                                                                       <  2:3932/251‑1 (MQ=255)                                                                                                            aaaTGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑Tg                                                                                                                                  <  1:342415/251‑1 (MQ=255)                                                                                                                                        cAGCGTGATCAACGGGTTAAAAGAAGAAACTCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCAGCCCGCGAACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGGGCTGGGGCTTGGCTATCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAATCCGCAAACGCACAGATCGCTGGAGTGA‑GTAAATCGCCCTCTTCGGTGCACtat                                                                                                      >  2:315498/1‑249 (MQ=255)                                                                                                                                              gATCAACGGGATAAAAGAAGAAAATCAGAACCGGAAAAACGAGCGGATGGTCGCGAAGAAAAAGTTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGAATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTTCTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCttat                                                                                                 <  1:358723/250‑1 (MQ=255)                                                                                                                                                     gggTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCGGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCGGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGa                                                                                         <  1:188138/251‑1 (MQ=255)                                                                                                                                                          aaaaGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATtg                                                                                     <  1:65762/250‑1 (MQ=255)                                                                                                                                                           aaaGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATtgtg                                                                                   <  1:362952/251‑1 (MQ=255)                                                                                                                                                                        aGAAACTGCAAAACGAGCTGATTGCCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGGGCTGGGGCTTTGCTTTCCGCTCGGGCTGGGACCGCCGCACCCGTTCCCAAGCCGCCAACGCCAAGATCGCTGGA‑TTAACTAAAACCCCTTCTTTGTTGAACTGTCTTATTATTATGAATT‑TGTTATTTGGCGAAgg                                                                     >  2:43982/1‑251 (MQ=255)                                                                                                                                                                                            aTTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCCAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGaa                                                                                        >  2:303471/1‑213 (MQ=255)                                                                                                                                                                                            aTTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGaa                                                                                        <  1:303471/213‑1 (MQ=255)                                                                                                                                                                                               gTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCt                                              >  2:55808/1‑251 (MQ=255)                                                                                                                                                                                                        aaaaaGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTTATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGGTTTATCTGGTCggtgg                                     >  2:370934/1‑251 (MQ=255)                                                                                                                                                                                                           aaGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTACACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGc                                  >  1:314186/1‑251 (MQ=255)                                                                                                                                                                                                               tCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTTTGGTACTGCCGAACCTGATCCCAAGCCGCAAACGCAAAGATCGGTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGGTGATGGCGTGAAGGATTATCTGGTCGGTGGTTCTGtt                              >  2:173748/1‑251 (MQ=255)                                                                                                                                                                                                                cGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGTTGATGGCGTGAAGATTTATCTGGTCGGTGGGGCTGTTc                             >  1:68787/1‑251 (MQ=255)                                                                                                                                                                                                                cGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTc                             <  2:146741/251‑1 (MQ=255)                                                                                                                                                                                                                             gTACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGTCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCCAACGCAAAGATCGCTGGA‑TTAACTAAATCGCCTTCTTCGTTGCACTTTCTTTTTATTTGGGATTGTGT‑ACTTGACGAAGGGATGATGGCGTGAAGATTTATATGGTCGGTGGGGCTGCTCGGGATGCATTGtt                >  2:74606/1‑251 (MQ=255)                                                                                                                                                                                                                              tACAGCTGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAATGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTTTTATTAGGAATTGTGT‑ATTTGACGAAGGGGTGATGGCGTGGAGGTTTATTTGGTCGGTGGTGCTGTTCggggtggcttgttt               >  2:262956/1‑240 (MQ=255)                                                                                                                                                                                                                                    tGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTTGGGCtt         >  1:261236/1‑250 (MQ=255)                                                                                                                                                                                                                                    tGGATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCt          >  1:197696/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggATGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTAc        >  1:299967/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTGACAAACAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCg      <  2:197696/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaCAGCGCACCACCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCa  >  1:200836/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               CACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAACGGGTTAAAAGAAGAAAATCAGAAACTGAAAAACGAGCTGATTGTCGCGCAGAAAAAGGTCGATGCCGCCAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGA‑TGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGT‑ATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCA  >  NC_000913/3201464‑3201948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑