Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,029,986	T→C	I316V (ATT→GTT)	ygfT ←	putative oxidoreductase YgfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,029,986	0	T	C	100.0%	87.2 / NA	32	I316V (ATT→GTT)	ygfT	putative oxidoreductase YgfT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/11);  total (21/11)

AAGTTAAATCGCTAAAGGTAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAATCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGCAAAGTTCTGCCGCTTCAATAATCTTTCCTT  >  NC_000913/3029752‑3030219                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           aaGTTAAATCGCTAAAGGTAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCg                                                                                                                                                                                                                           >  2:316646/1‑251 (MQ=255)          cGCTACAGGAAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACgggg                                                                                                                                                                                                                   >  1:307829/1‑250 (MQ=255)           gCTAAAGGTAATATCGCGGCCAATTTCACAGTTAAGCTGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGa                                                                                                                                                                                                                  >  2:190687/1‑250 (MQ=255)               aaGGTAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGaacaac                                                                                                                                                                                                             >  1:51123/1‑251 (MQ=255)               aaGGTAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTAGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGaacaac                                                                                                                                                                                                             >  1:298340/1‑251 (MQ=255)                 ggTAATACCGCGGCCAATTTCACATTAAAGATGAAAGGCGATCCCCATTGGGGTGAATATCTCCCGCCGCGGGCTAAATACCGTTTT‑ATCGAGTTTAAAAGGAGAAATGCAAAAA‑GTCAGCATCCCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAAAATACCAGCAC‑CCCCTACCCCTGCAGGCCCAGCGCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACtt                                                                                                                                                                                                           <  1:316646/251‑1 (MQ=255)                            ggCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGGGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACAGCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACACCTTTGCTGACATCg                                                                                                                                                                                                >  2:330553/1‑251 (MQ=255)                                  tttCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGc                                                                                                                                                                                          >  2:257797/1‑251 (MQ=255)                                           aaaaaagAAAGTCGATCCCCATGGCGGTGAATTACCCTCGCCGGTGTCTAAATACCTTTTT‑CTCGAGTTAAAAAGGAGAAATGCAAAAA‑GTCACCCT‑CCCCCAATTTTCT‑GGATGGCGATAAAAAACATCGCCCTGACCCCCTCC‑G‑CGCCCCAAAATACCAGCAC‑CCCAGACCCCGGCAGCCCAAGCGCCACCCTCC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATc                                                                                                                                                                                 <  2:164718/245‑1 (MQ=255)                                                         aTTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGCAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCACAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCCACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGtctc                                                                                                                                                                   >  2:163125/1‑248 (MQ=255)                                                         aTTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATc                                                                                                                                                                   >  1:230058/1‑251 (MQ=255)                                                             cgatgggggtaaaTATCTCTCGCCGCTGGCTAAATACCGTTTT‑AGCGAGTTGGAAGGGAGGAA‑GCCAAAAAGCCACCAACCCGCCAATTT‑CG‑GGAGGGCGACAAAAGACACCCACCT‑ACCCCCCGCCG‑CGCGCAGGAATACCACCCC‑CCCCTACCCCGGCGGGCCAAGCGCAAACCCCC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTg                                                                                                                                                               <  2:70848/240‑1 (MQ=255)                                                               aTTGCCGTGAAAATGTCTCGCCGCTAGCTTCTTACCGGGTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑CTCAGCATAACGGCAATTT‑CT‑GGATGGTGATCAAAGACATCGCCCTGAACTCCTGC‑G‑CGCGACAGAATATCAGAAC‑ACCCTAACCCTGCAGGCCCGGCGCCAACCACC‑GCCGCTTTTTCGCGAAGGGGAACACCTTTGCTGACATAGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGAt                                                                                                                                                             >  2:156383/1‑251 (MQ=255)                                                                   gggtaattaccgctccccgcTGGCTAAAT‑CCGTTTTCTTCGATTTTGAAAGAAGGAATCCGAAAG‑GTAACCAAACCCCCAATTT‑CT‑GTATGGCGATCAAAGACACCGACCGGACCCCCGGC‑C‑CGCCCAAGAATATCACCAC‑ACCCTACCCCTCCAGTCCC‑GCCCCAACCCCCCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAg                                                                                                                                                         <  1:92732/235‑1 (MQ=255)                                                                               ctcGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGAtt                                                                                                                                              >  2:77798/1‑250 (MQ=255)                                                                                                  cGTTTT‑ATCGAGTTGGAAAGGAGGAATGCAAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGAGGGCGAACAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATACCAGCAC‑CCCCTACCCCTGCAGCCCCAGCGCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCg                                                                                                                          <  1:108262/251‑1 (MQ=255)                                                                                                           cggttttGAAAGGAGGAA‑GCCAAAATGCCAGAATCCCGCCATTTT‑AG‑GTATGGCTATCAAAGACATCGCCCTGATCACCGGC‑G‑CGGGAAATAATCTCA‑CACCACCCTACCCCTGCAGACCCATCCCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATc                                                                                                                   <  1:330553/246‑1 (MQ=255)                                                                                                           cGAGTTTAAAAGGAGAAAACCCAAAA‑GTAAGCATCCCCCCAATTT‑CG‑GGATGGCAATAAAAGACATCGCCCTGACCTCC‑GC‑GGCGCCCAAGAATATCAGCCC‑CCCCTAACCCGGCAGGCCCAGCGCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATc                                                                                                                   <  1:299453/250‑1 (MQ=255)                                                                                                                           aaTGCCAAAA‑TTCAGCATCCCGCCA‑TTT‑CTTGGATGGCGAAAAAAAAAACCGACCTGAACCCCTGC‑G‑CGCGCCAAAATATCAGCAC‑CCCCAACCCCGGCGGCCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATgcgc                                                                                                  <  1:70479/251‑1 (MQ=255)                                                                                                                                cAAAA‑GTCAGCATACCGCCACTTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGCTCTTTCAAAGTACATGCGCCTTc                                                                                              >  2:358241/1‑250 (MQ=255)                                                                                                                                  aaa‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTcac                                                                                            >  1:56781/1‑250 (MQ=255)                                                                                                                                                               aTGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGc                                                                 >  2:290020/1‑251 (MQ=255)                                                                                                                                                                      tCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAt                                                          >  2:285641/1‑251 (MQ=255)                                                                                                                                                                           gACATCGACCTGAACTCCTGC‑G‑CGCGCCAGCATAACAACAC‑ACACTACCCCTGCAAGAACAGCGCCAACCACC‑GCCACATTTTCGCAACGCAGAACAACTTTGATGACATCGGCACGCAAGCCCATCGCCAGCGCGGTCTCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCACGAGTGATCTTTCAAAGTACATGCGCCTTCACAAACACGGTCCTGAGGACATACCCTGCCGCAAATATCTg                                                     >  1:152348/1‑251 (MQ=255)                                                                                                                                                                                  aCCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAgg                                               >  1:42556/1‑250 (MQ=255)                                                                                                                                                                                   ccTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGa                                              >  1:175984/1‑250 (MQ=255)                                                                                                                                                                                        ccTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑CCCCTACCCCGGCAGCCCCAGCCCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAActggt                                        <  1:6038/250‑1 (MQ=255)                                                                                                                                                                                                 ccgcccccgaaacatCACCAC‑CCCCTACCCCTGCAGCCCAAGCCCAAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCtggtgg                                 <  1:13192/238‑1 (MQ=255)                                                                                                                                                                                                   cgcgcCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGc                                >  1:154501/1‑250 (MQ=255)                                                                                                                                                                                                            atatCAGCAC‑ACCCTCACCCTGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGCAAAGTTCTGc                      >  1:107296/1‑251 (MQ=255)                                                                                                                                                                                                                         ccaaccccTGCAGCCCCAGCCCCACCCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGCAAAGTTCTGCCGCTTCaataat          <  1:115701/246‑1 (MQ=255)                                                                                                                                                                                                                                 tGCAGGCCCAGCGCCAACCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGCAAAGTTCTGCCGCTTCAATAATCTttcctt  >  2:131383/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           AAGTTAAATCGCTAAAGGTAATATCGCGGCCAATTTCACAGTTAAGATGAAAGTCGATTCCCATTGCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTT‑ATCGAGTTTGAAAGGAGGAATGCCAAAA‑GTCAGCATACCGCCAATTT‑CT‑GGATGGCGATCAAAGACATCGACCTGAACTCCTGC‑G‑CGCGCCAGAATATCAGCAC‑ACCCTAACCCTGCAGGCCCAGCGCCAATCACC‑GCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCTGGTAAGGAACTGGTCTGGTGGCAAAGTTCTGCCGCTTCAATAATCTTTCCTT  >  NC_000913/3029752‑3030219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑