Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,659	T→C	K192K (AAA→AAG)	ygbI ←	DNA‑binding transcriptional repressor YgbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,659	0	T	C	100.0%	103.2 / NA	34	K192K (AAA→AAG)	ygbI	DNA‑binding transcriptional repressor YgbI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/9);  total (25/9)

GTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGAC  >  NC_000913/2860416‑2860890                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        gtCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCATGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATAGTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCGCTGGCAATCGCCCGCTTCACCg                                                                                                                                                                                                                                  <  1:162946/251‑1 (MQ=255)         cttcttCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACtttt                                                                                                                                                                                                                          >  2:278100/1‑250 (MQ=255)           tcttctGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGCTCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCTCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCCCCGTGACTTTATc                                                                                                                                                                                                                        >  1:287106/1‑251 (MQ=255)                tgtgAAGCATGAGTAACCCGGAGTTATTGCAGGCCATTCTTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACACGCTTAATGGTAACTCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCg                                                                                                                                                                                                                   >  2:104886/1‑251 (MQ=255)                                    gtgtTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGc                                                                                                                                                                                               >  1:364080/1‑251 (MQ=255)                                      gtTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGcac                                                                                                                                                                                             >  2:213399/1‑251 (MQ=255)                                       ttATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGcac                                                                                                                                                                                             >  1:21537/1‑250 (MQ=255)                                                   attattCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGAt                                                                                                                                                                                <  1:80780/251‑1 (MQ=255)                                                      attCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATAAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGAATTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGTTGc                                                                                                                                                                              >  1:352611/1‑250 (MQ=255)                                                         cATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGa                                                                                                                                                                           >  2:326044/1‑250 (MQ=255)                                                            ttttCGCTACCAGCAAAGAGAGATCCTGCTTCACTAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGCTCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGTCCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAAt                                                                                                                                                                        >  2:256386/1‑250 (MQ=255)                                                                            agagagATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGatcaatc                                                                                                                                                        >  2:116388/1‑250 (MQ=255)                                                                                       gCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTgcg                                                                                                                                             <  2:142412/250‑1 (MQ=255)                                                                                                     cgACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGc                                                                                                                              >  2:100576/1‑251 (MQ=255)                                                                                                                 tcgggaaaaCCGTCGTCTGTAATAATATGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGac                                                                                                                  >  2:149152/7‑251 (MQ=255)                                                                                                                       aaaCCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAgg                                                                                                             >  2:100896/1‑250 (MQ=255)                                                                                                                           cgtcgtCTGTAATAATCTGATCAAACGCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACGCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTGTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATTCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCAGGGTCGCAGCGGCTTCCCCGACACAGGAACgg                                                                                                        <  1:217066/251‑1 (MQ=255)                                                                                                                                     aataatCTGATCAACCTCGCTTAATGGTAACGCCAGCCATGTCTCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCTTGACGTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACGCCATGACGATGCAGAAAGAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGTTCGCAGCGGCTTCCCCGACACAGGAACGGTTTACCCGa                                                                                               <  1:149152/250‑1 (MQ=255)                                                                                                                                     aataatCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTACCGac                                                                                              >  1:86071/1‑251 (MQ=255)                                                                                                                                             gATCAAACGCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACGGGCAAGCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCGCACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCAGGGTCGCAGCGGCTTCCCCGACACAGGAACGGTGTTCCCGACACACTGc                                                                                       <  2:17820/250‑1 (MQ=255)                                                                                                                                                 aaaCTCGTTTAATGGTAACGCCAGCCATGTCGCCACTTGACCAGATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGTCGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGTCTGATCAATCATCAGGCTGTGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACCCACTGCCCCGc                                                                                  <  1:196141/251‑1 (MQ=255)                                                                                                                                                    cTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGcc                                                                                 >  1:368603/1‑249 (MQ=255)                                                                                                                                                                aaccGCCAGCCATTTCGCCCCCTACCCATATTTGGTCGCATCACAGACAAAAACCCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCGGGCTTAGAAATCCCCCGCACACTCCATGACGAGGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCAGGTTCGCAGCGGCTTCCCCGACAAAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATtg                                                                   <  2:201706/248‑1 (MQ=255)                                                                                                                                                                    gccagccaTGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGc                                                                >  1:366938/1‑250 (MQ=255)                                                                                                                                                                      cagccaTGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAAt                                                             >  1:276860/1‑251 (MQ=255)                                                                                                                                                                       agccaTGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAAtt                                                            >  2:155203/1‑251 (MQ=255)                                                                                                                                                                                               ttCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGc                                    >  1:257497/1‑251 (MQ=255)                                                                                                                                                                                                       aTCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACg                             >  2:90009/1‑250 (MQ=255)                                                                                                                                                                                                        tCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGa                            >  1:94422/1‑250 (MQ=255)                                                                                                                                                                                                         cacaGCCCAAAACTCGCGGGCGGCTGGCACTGGCAATCGCCCGCTTCCCCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACCCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCAGGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGaaa                          <  2:366938/251‑1 (MQ=255)                                                                                                                                                                                                           caGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGCGCAAGTAGTCCGCAATAACGAAATc                        >  1:54602/1‑251 (MQ=255)                                                                                                                                                                                                               ccAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGttt                    >  2:186701/1‑251 (MQ=255)                                                                                                                                                                                                                                ctggcactggccATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAACTAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTg    >  2:23894/1‑250 (MQ=255)                                                                                                                                                                                                                                 tggcactggcaATCGCCCGCTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGAc  >  1:8731/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        GTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTCGCAGCGGCTTCCCCGACACAGGAACGGTTTTCCCGACACACTGCACCGCCAGTGTGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGAC  >  NC_000913/2860416‑2860890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑