Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,943	G→A	G103D (GGC→GAC)	tktB →	transketolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,943	0	G	A	100.0%	114.8 / NA	33	G103D (GGC→GAC)	tktB	transketolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

CCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTT  >  NC_000913/2579697‑2580175                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         ccAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACtat                                                                                                                                                                                                                                      >  2:122547/1‑251 (MQ=255)          gTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGc                                                                                                                                                                                                                             >  2:125434/1‑251 (MQ=255)                  ggCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAac                                                                                                                                                                                                                     >  2:70275/1‑251 (MQ=255)                  ggCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCGGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAac                                                                                                                                                                                                                     <  2:95833/251‑1 (MQ=255)                                  tGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCtt                                                                                                                                                                                                     >  2:126351/1‑251 (MQ=255)                                         tatTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCAAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACa                                                                                                                                                                                                >  1:163856/1‑249 (MQ=255)                                               cGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTgg                                                                                                                                                                                        >  1:82351/1‑251 (MQ=255)                                                    tGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAAc                                                                                                                                                                                   >  2:131142/1‑251 (MQ=255)                                                         tgGAACGATTTTCTTAACCATAACCATACCGACACAACCTGGTATGATCACGACCACTTTATTCTTTCCAACGGTCAAGCGCAGATGCTGCTCTACAGTTTGCTAAATCTGACCGGTTACGAACTGCCGCTGGTAGAACTGAAGAACTTCCGTCAGTTGCACTCGAAAACCCCCGGCTACCCGGAGATTGACTATACGCCAGGCGTTCAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACTCCGt                                                                                                                                                                              >  2:312653/1‑251 (MQ=255)                                                              cGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGCTCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGc                                                                                                                                                                         >  2:73505/1‑251 (MQ=255)                                                                                      cGACCCAACCTGGTCTGATAGCGAACGCTTTATTCTTTCCAACGGTCACGCGCCGATGCTCCTCTACAGTTTGCTCCATCTGACCGGTTACGACATGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTacaccgg                                                                                                                                                 >  2:45096/1‑248 (MQ=255)                                                                                       gACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTggc                                                                                                                                                <  1:121706/251‑1 (MQ=255)                                                                                            acccTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCCCGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGCCCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCAATCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGgcgc                                                                                                                                           <  2:262535/249‑1 (MQ=255)                                                                                             aCCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACAGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGgcgc                                                                                                                                           >  1:222585/1‑250 (MQ=255)                                                                                                      gagcgcgGCCGCTTTATTCGTTCCAACGGTCACGCGTCGATGTTGCTCTACAGTTTTCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAATCCCCAGGCCACCCGGATATTGACTATACGCCGGGCTTTGAAATCTCCACCTGCCCGCTTGGACGAGGTGTGGGGATCGCGGTCGGGGGTGCGGTAGCAGAGCGTACACTGGCGGCGCAGTTTAAcca                                                                                                                                 <  1:41423/248‑1 (MQ=255)                                                                                                          gcgACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAccagcc                                                                                                                              >  1:297503/1‑250 (MQ=255)                                                                                                           cgACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGCCGCAGTTTAAccagcc                                                                                                                              >  1:124555/1‑249 (MQ=255)                                                                                                                   tgaTTCTTTCAAACGGTCACGCGTCGATGCGGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACGGAAGAACTGCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATg                                                                                                                      <  2:295781/247‑1 (MQ=255)                                                                                                                    tATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATgag                                                                                                                    <  1:70275/250‑1 (MQ=255)                                                                                                                     aTTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATgaga                                                                                                                   <  1:122547/250‑1 (MQ=255)                                                                                                                                    cACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCa                                                                                                    <  1:125434/250‑1 (MQ=255)                                                                                                                                             aTGCTGCTCTACGGTTTGCTCCATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGtt                                                                                          <  2:364158/251‑1 (MQ=255)                                                                                                                                               gctgctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGttt                                                                                         >  1:361834/1‑250 (MQ=255)                                                                                                                                                  cggttgatgTTTGCGACATAGGACCGGTTGCGACCTGCCGCTGGAAGAACAGAAGAACTTCCGGCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATGCGCCAGGCGTTGAAACCCCCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGTCGTCGCAGTTTAACCAGCCATACCATGAGATCGTCGATCACTTCACCTATGTGGTTATgg                                                                                     <  2:222196/243‑1 (MQ=255)                                                                                                                                                          gTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                                                >  1:45699/1‑232 (MQ=255)                                                                                                                                                          gTTTGCTACATCGGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                                                <  2:45699/232‑1 (MQ=255)                                                                                                                                                                                      gCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAg                                                 <  1:16503/251‑1 (MQ=255)                                                                                                                                                                                                   ctgaagaactTCCGTCAGTTGCATTCGAACACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCCTGCGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGACGTCTGTTCGCTggc                                    >  2:331404/1‑251 (MQ=255)                                                                                                                                                                                                                  cAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTGGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                      <  2:246898/250‑1 (MQ=255)                                                                                                                                                                                                                      tGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTggg                  <  1:278928/250‑1 (MQ=255)                                                                                                                                                                                                                                     cAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCCCCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGCCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt  <  2:124555/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGCAGGGATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt  >  2:145461/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt  <  1:131142/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         CCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTT  >  NC_000913/2579697‑2580175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑