Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,475	T→C	Q223Q (CAA→CAG)	yfaP ←	DUF2135 domain‑containing protein YfaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,475	0	T	C	100.0%	134.3 / NA	39	Q223Q (CAA→CAG)	yfaP	DUF2135 domain‑containing protein YfaP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/25);  total (14/25)

GGCAACACTGTGCATTGGCGGCGGTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCC  >  NC_000913/2327233‑2327713                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ggCAACACTGTGCATTGGCGGCGGTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCgg                                                                                                                                                                                                                                         <  2:163332/250‑1 (MQ=255)                      cggTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAATCAATAAAAACTCCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTgcg                                                                                                                                                                                                                   <  1:189873/251‑1 (MQ=255)                      cggTCAGGGAATTGCGATGGTGATGGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGAGGTGGTTAATTCCGTTTCGCTgcg                                                                                                                                                                                                                   <  2:7242/251‑1 (MQ=255)                                        ggTGATTGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGat                                                                                                                                                                                                 >  1:241654/1‑251 (MQ=255)                                              tGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGTTAACCCCATTAATCACCAGTCAAAGCTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTTTCATTTACCGATCCTTCATCGGTGATCAGCGTCAGCTGTGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTGGATATaca                                                                                                                                                                                            <  2:168045/250‑1 (MQ=255)                                                       gAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAgcg                                                                                                                                                                                  <  1:116757/251‑1 (MQ=255)                                                                            cGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCAt                                                                                                                                                             >  2:83907/1‑251 (MQ=255)                                                                                      aGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAAtc                                                                                                                                                   <  2:1171/251‑1 (MQ=255)                                                                                               gtgtTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCATCATTACGCAGCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCg                                                                                                                                          <  1:205699/251‑1 (MQ=255)                                                                                                     tCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTGTCGCTGCGCCCACCATAGTAGTTGTTATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTAccc                                                                                                                                     <  1:166440/250‑1 (MQ=255)                                                                                                             aTCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGAc                                                                                                                            >  1:114313/1‑251 (MQ=255)                                                                                                                  cGGCGAAGGTAACCCCATTAATCGCCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCa                                                                                                                       <  2:175092/251‑1 (MQ=255)                                                                                                                       aaGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATcc                                                                                                                  >  1:263316/1‑251 (MQ=255)                                                                                                                        aGGTAACACCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATcc                                                                                                                  >  1:95794/1‑250 (MQ=255)                                                                                                                         ggTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAg                                                                                                                <  2:30552/251‑1 (MQ=255)                                                                                                                            aaCCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCGTGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCAGAGTAGTGGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCGCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGc                                                                                                             <  1:58300/251‑1 (MQ=255)                                                                                                                               cccATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGcacc                                                                                                          >  1:201316/1‑251 (MQ=255)                                                                                                                                     aaTCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGtt                                                                                                    <  1:279605/251‑1 (MQ=255)                                                                                                                                     aaTCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGt                                                                                                     <  2:68081/250‑1 (MQ=255)                                                                                                                                          ccAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTc                                                                                                >  1:43777/1‑250 (MQ=255)                                                                                                                                           cAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTc                                                                                                >  1:362941/1‑249 (MQ=255)                                                                                                                                            aGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGc                                                                                             <  2:168201/251‑1 (MQ=255)                                                                                                                                                aaaaCTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACgg                                                                                         <  1:142637/251‑1 (MQ=255)                                                                                                                                                       ttCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGCGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTAcc                                                                                  >  1:183776/1‑251 (MQ=255)                                                                                                                                                                  cAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCgt                                                                       <  1:83907/251‑1 (MQ=255)                                                                                                                                                                        gCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGc                                                                 >  1:132977/1‑251 (MQ=255)                                                                                                                                                                            gCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATc                                                             <  2:362941/251‑1 (MQ=255)                                                                                                                                                                             cATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCg                                                            <  2:114313/251‑1 (MQ=255)                                                                                                                                                                              aTTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCCGGCGTGTTCGCCATCgg                                                           >  2:314147/1‑251 (MQ=255)                                                                                                                                                                                tACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCggg                                                          <  1:74464/250‑1 (MQ=255)                                                                                                                                                                                       cGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTaacaa                                                  <  1:205992/251‑1 (MQ=255)                                                                                                                                                                                                       tCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAaggtcgaggt                                   <  2:43777/250‑1 (MQ=255)                                                                                                                                                                                                               ctcATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGt                          >  2:341393/1‑251 (MQ=255)                                                                                                                                                                                                                 cATTGACCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTcc                        <  2:105407/251‑1 (MQ=255)                                                                                                                                                                                                                      aCCGATCCTTCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATc                   <  1:23146/251‑1 (MQ=255)                                                                                                                                                                                                                              ttCATCGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACg              >  1:91896/1‑248 (MQ=255)                                                                                                                                                                                                                                   cGGTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCATCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAAc       <  2:263316/250‑1 (MQ=255)                                                                                                                                                                                                                                    ggTGATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCa     <  1:103359/251‑1 (MQ=255)                                                                                                                                                                                                                                       gATCAGCGTCAGCTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGcc  >  2:356955/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               GGCAACACTGTGCATTGGCGGCGGTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAATCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAGTCAAAACTTTTCACCAGCGTCAGCTCGCCAGCATTACGCATCGGTACAATAAATGTTTCCTGTTTCTCATTGACCGATCCTTCATCGGTGATCAGCGTCAGTTGGGCGGTGGTTAATTCCGTTTCGCTGCGCCCACCATAGTAGTTGATATACACCTGATAGCGCCCGTGAATTGGCGCGGGCATGGCGAAAATCTCGGGTCCGTACCCCGTCGTGACATCCATATCCAGTGCACCACTGTTTTTCAGCACGGTGTTACCGTACCAGGCGTGTTCGCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCC  >  NC_000913/2327233‑2327713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑