Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,207	A→C	intergenic (+142/+231)	yohP → / ← dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,207	0	A	C	100.0%	48.4 / NA	18	intergenic (+142/+231)	yohP/dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/7);  total (11/7)

CTCACAAATAAAGGAACGCCAATGAAAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACT  >  NC_000913/2228961‑2229429                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               cTCACAAATAAAGGAACGCCAATGAAAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGcccccc                                                                                                                                                                                                                            >  2:312365/1‑251 (MQ=255)                       tGAAAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATcg                                                                                                                                                                                                      >  1:271437/1‑251 (MQ=255)                        gAAAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCGCTGGCGCAAGGAGCGCGCAGGGGGCGGCCGATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATcg                                                                                                                                                                                                      <  1:57831/250‑1 (MQ=255)                         aaaaTTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                                                                                                                                    <  1:115902/251‑1 (MQ=255)                                                                                                  tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                                                                                                                           >  1:89062/1‑251 (MQ=255)                                                                                                            ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCCGccc                                                                                                                  >  1:325688/1‑250 (MQ=255)                                                                                                               attaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCCGGGCGGGCGCCGGGTAACAACCCTGCAAAAACCACCCTCACCCcacac                                                                                                              >  2:6832/1‑250 (MQ=21)                                                                                                               attaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCAGGGCTCTGCCGAACAAAATCGCAGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCCCGAGGTAACACCCCTGTAAAAAGCGcccccaccccccc                                                                                                               >  2:263989/1‑240 (MQ=21)                                                                                                                     gTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGCGGGAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgt                                                                                                        >  1:319152/1‑251 (MQ=255)                                                                                                                      tcatcaGGTCCGAAAATAACGAGAATATTTCAGTATCTCATCCTGTTGCGCTCCTGTCATGTACATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGATCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtggg                                                                                                       >  1:164641/1‑249 (MQ=255)                                                                                                                                       aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTTCCCCGGCCTTc                                                                                       <  2:1310/250‑1 (MQ=255)                                                                                                                                           tgcatatTTCGGTCTCTCATCCTCCTACGCGCCTGTCATGTGCATTGCTTCATATAATCACTCGCGCACAGAACCCGCAGGGGGCGGACAATCGCCGCCGCCCCATGCCCCCCCGCGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCATCGACCTATCCCTTACGACTACCGAGTCCCCCGCGCGATCCAAACCCTGTAAAACGCCCACTCAAACCCCATCCCTGTGGGCTGCCCCCGCCTTCAgggg                                                                                  >  2:32971/4‑250 (MQ=9)                                                                                                                                                          ctcATCCTGTTGGGCTCCTGTCAGGTGCATTGTTTCATATAATCACTGGCGCAATGAGCGCGCATGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGGACAAAATCGCCGCTGCTCGTGGCCCTCGGCTTGTCCCTTACGGCGACCGTGTCGGGCGCGAGGTAGCAGCCCTGTAAAAGTCGCCGTCAGCCGGCATCCATGTTGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCaa                                                                      <  1:211053/248‑1 (MQ=14)                                                                                                                                                          ctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAAt                                                                     <  1:51451/249‑1 (MQ=255)                                                                                                                                                               ccTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCCGGTCGGGGGCGAGGGAACATCCCTGTAAAAACCGCCCTCAGCCCCCATCCACGTGGGCTGCCCCGTCCTTCAGGGAACGCCTCGGCCATTTTGAcc                                                              >  2:65977/1‑250 (MQ=17)                                                                                                                                                                                                  aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGAGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGACTATTGATAAAGAGc                           >  1:183960/1‑251 (MQ=255)                                                                                                                                                                                                          gcgcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACAt                   <  2:271437/251‑1 (MQ=255)                                                                                                                                                                                                           ggcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACAtt                  <  1:17194/250‑1 (MQ=255)                                                                                                                                                                                                                            gggCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACCTTGTCAAAAAACATccct  >  1:132059/1‑247 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               CTCACAAATAAAGGAACGCCAATGAAAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACT  >  NC_000913/2228961‑2229429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑