Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,945	T→C	N309S (AAC→AGC)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,945	0	T	C	100.0%	71.9 / NA	23	N309S (AAC→AGC)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

AACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTT  >  NC_000913/2223719‑2224179                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aaCAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGAAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCGGTCTGCCccgccgccc                                                                                                                                                                                                                    >  2:240038/1‑250 (MQ=255)     gATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCAt                                                                                                                                                                                                                >  1:256052/1‑251 (MQ=255)                    aCAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGTAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGCGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCttttt                                                                                                                                                                                                  <  2:122077/250‑1 (MQ=255)                                 gcgcAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTc                                                                                                                                                                                    >  1:6673/1‑251 (MQ=255)                                          ccTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACgctgct                                                                                                                                                                           >  2:52574/1‑251 (MQ=255)                                                        cGTCTGTCATTTGTATAAAGCGCCGGAGACGTTGCAGAAGTTCTATCTCGAGAACGATCCGAACAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCTTCTTCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCAt                                                                                                                                                             <  1:225829/251‑1 (MQ=255)                                                           cGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCa                                                                                                                                                           <  2:267145/250‑1 (MQ=255)                                                                    gTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGt                                                                                                                                                 >  1:272799/1‑251 (MQ=255)                                                                                  agaCGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTAc                                                                                                                                   <  1:167475/251‑1 (MQ=255)                                                                                               gTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGc                                                                                                                      <  1:161446/251‑1 (MQ=255)                                                                                                                       cAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCgg                                                                                                                                                                      <  1:45723/179‑1 (MQ=255)                                                                                                                       cAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCgg                                                                                                                                                                      >  2:45723/1‑179 (MQ=255)                                                                                                                                gAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGt                                                                                      <  1:11059/250‑1 (MQ=255)                                                                                                                                             ggTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTTGAGTAAAAAGTCCGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTATTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAgc                                                                        <  2:257869/251‑1 (MQ=255)                                                                                                                                                  aacaaGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGAGGGCAGACTACCGCCATCCGAATTCACTTAATCGCTCTGGGCCGTCTGCCCCGCCGCCGCCCTTTGGGCGGTTTGTTGTTTTTTATAGCTCAACGCAGCTGCCGGCACAGGCATCACTTTACCGGTTTCAAGCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGcctc                                                                   <  2:180873/247‑3 (MQ=255)                                                                                                                                                  aaCCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCAc                                                                   <  2:6673/251‑1 (MQ=255)                                                                                                                                                                  aaCTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATa                                                   <  2:265832/251‑1 (MQ=255)                                                                                                                                                                       gCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCCCTTAATCGCTCTTGGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTAGAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGt                                              <  2:317897/251‑1 (MQ=255)                                                                                                                                                                          ggAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGAt                                                    <  2:215703/242‑1 (MQ=255)                                                                                                                                                                          ggAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGAt                                                    >  1:215703/1‑242 (MQ=255)                                                                                                                                                                                                       agTATCGCCATCCGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTCTTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCCTTgg              >  2:172337/1‑251 (MQ=255)                                                                                                                                                                                                                 cccGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGt    <  2:126796/250‑1 (MQ=255)                                                                                                                                                                                                                   cGAATTCACTTAATCGCTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGttt  >  2:13731/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           AACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAAACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTT  >  NC_000913/2223719‑2224179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑