Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,208,509	T→C	S181S (AGT→AGC)	yehP →	VWA domain‑containing protein YehP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,208,509	0	T	C	100.0%	78.8 / NA	25	S181S (AGT→AGC)	yehP	VWA domain‑containing protein YehP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

CGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGTACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTC  >  NC_000913/2208281‑2208749                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTTGTGTCCGCGATCCCCCTCGCCGTTCCTTTATTTCACCGGCGCGGAACTTTTATTTTCAAAGCACTCTGCGCGCCACCCTGCaa                                                                                                                                                                                                                             >  2:301214/1‑250 (MQ=255)                             gAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGta                                                                                                                                                                                                <  1:42655/251‑1 (MQ=255)                                       tACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCCAGTTGTATATCGAATcc                                                                                                                                                                                      >  1:141329/1‑251 (MQ=255)                                        actcgcTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCACGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATc                                                                                                                                                                                       <  2:115693/246‑1 (MQ=255)                                                    gCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCCCTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCCCGCCAACCTGCAACACTGGCACCCCCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTaaa                                                                                                                                                                          >  1:176384/1‑249 (MQ=255)                                                                      gaACCCCGAAGTACTGGCTGCCGCCCGCCGCATAGTGTGCCCGGATGTTGAAGACATTATGGCTCGACTGGCAAAGGGTGTTTGTCCGACTTTTTCTGGTGGCCGCGATCGCAGCCGCCGGTTATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCCACTTGTATAACGAATCCCCCCGCTTTAACAGCCGCATTAAACCCCaa                                                                                                                                                        >  1:355450/1‑250 (MQ=255)                                                                            cGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAAc                                                                                                                                                                          <  2:273852/226‑1 (MQ=255)                                                                            cGAAGTACTGGCTGCCGCCAGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGGCAGGCTTTTTCTGGGGTCCGCGCTCGCCGGCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATAGAATCCCCCCGCTTTAAc                                                                                                                                                                          >  1:273852/1‑226 (MQ=255)                                                                             gAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACa                                                                                                                                                <  2:62835/251‑1 (MQ=255)                                                                                                gccgGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGCTCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTTCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACCGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTg                                                                                                                             >  1:375481/1‑251 (MQ=255)                                                                                                    gATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTg                                                                                                                         >  1:282157/1‑251 (MQ=255)                                                                                                                   tgttgAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCg                                                                                                           <  1:217156/250‑1 (MQ=255)                                                                                                                                                       tCGTCAGGCTTTGTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTGGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATggcg                                                                       <  2:310255/250‑1 (MQ=255)                                                                                                                                                                  tttCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGGTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGGCGATTCGGTGATCCACTCTGCGGTGATGGCGGGCTGGTTGTgg                                                           >  1:3189/1‑251 (MQ=255)                                                                                                                                                                   ttCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGc                                                          >  1:3718/1‑251 (MQ=255)                                                                                                                                                                          gtCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTAc                                                    <  1:301214/250‑1 (MQ=255)                                                                                                                                                                                   cgccgtcgccgtTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTc                                           <  1:319981/250‑1 (MQ=255)                                                                                                                                                                                   cgccgtcgccgtTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTc                                           <  1:253989/250‑1 (MQ=255)                                                                                                                                                                                            cgtTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCCCCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGCTACACTCTGCGGTGATGGCGGCATGTTTGGGGCAGGTACCCggacctcgtccccctct                                 >  2:331166/1‑236 (MQ=255)                                                                                                                                                                                            cgtTCATTTATACCAAGGGTGCGAAAATTTGATTTGAAAAGCACCCGGCGCGCCGGCCTGCAACGCGCGCACCCGCACCACGGGAAGTTATATATCGAATCCCCCCGCTTTAACAGCCGCAGTAAACGCCAAAGCGAAGAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCt                                 <  2:89745/251‑1 (MQ=255)                                                                                                                                                                                                   ttATTCCACTGGCGCGAAACTTTGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCg                          >  2:35941/1‑251 (MQ=255)                                                                                                                                                                                                    tATTCCACTGGCGCGACACTTTGCTTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGt                         >  1:269362/1‑251 (MQ=255)                                                                                                                                                                                                                        ttGATTTCAAAAGCACTCTGCGCGGCAACCGGCAACGCTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCAGTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGa      <  2:27199/250‑1 (MQ=255)                                                                                                                                                                                                                        ttGATTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTg       <  2:355036/249‑1 (MQ=255)                                                                                                                                                                                                                           aTTTCAAAAGCACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGAGCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGAtctc  <  1:299151/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGTACTCTGCGCGCCAACCTGCAACACTGGCACCCGCAACACGGCAAGTTGTATATCGAATCCCCCCGCTTTAACAGCCGCATTAAACGCCAAAGCGAACAATGGCAACTGGTCTTACTGGTTGATCAAAGCGGATCGATGGTCGATTCGGTGATCCACTCTGCGGTGATGGCGGCCTGTTTGTGGCAGTTACCCGGCATTCGTACCCATCTGGTGGCGTTTGACACAAGCGTCGTTGATCTC  >  NC_000913/2208281‑2208749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑